Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 117918469

GI number: 117918469

Start: 11054

End: 12154

Strand: Direct

Name: dnaN [H]

Synonym: Shewana3_0010

Alternate gene names: 117918469

Gene position: 11054-12154 (Clockwise)

Preceding gene: 117918468

Following gene: 117918470

Centisome position: 0.22

GC content: 45.59

Gene sequence:

>1101_bases
ATGAAATTTTCAATTGATAGGGACGCCCTATTAAAGCCGCTTCAATTGGTTTGTGGTGCGGTAGAAAGACGCCATAACTT
GCCTATTTTGGCAAATCTCCTTGTAGAAGTTAGTGGTCACTCACTCAAAATGACAGGTACCGACCTCGAAGTTGAGTTAG
TGGGCCAGGCGGTCATTCATGGTGATATCGAAGAAGGTCGTACAACAGTTCCTGCTAAGAAACTGTTAGATATAGTTAAA
TCCTTACCCGAGCAAAGCGAGCTTAAGGTCGAGCAACAAGATAACAAATGGTTATTACGCTCTGGCCGTAGCCGTTTCTC
GCTGGCAACCCTGCCCGCAGAAGAATACCCCAATGTGGAAGCCTTTCAGGCGGAAATCGAATTCACCCTTAAGCAAGGCG
TGCTGAAGTCACTGATTGATGCGACTCAGTTTTCGATGGCGAACCAAGACGTTCGTTACTATCTGAATGGTTTATTGCTC
GAAACCGAAGGTAATGTGCTGCGTGCCATTGCTACCGATGGTCACCGCTTAGCCTTAAGTCACAGAACTATTGATGCGCA
ATTGCCTGAAAAACAAGTGATCGTGCCCCGTAAAGGCGTGATGGAAATGGCGCGTTTGTTGGAAGCCGATGATTTAGATA
TCGCAATTGCCATTGGTGATAATGCGATTCGTGCGACCACCAGCACCACAGTTTTTACCAGTAAATTAGTCGATGGTCGT
TTCCCCGATTATCGCCGTGTGTTACCTAAGGGCGGTGATAAAATCGTTATCGCCAGCCGTAACCACTTCAAACAAGCCTT
AACCCGTGCTTCTATTTTATCGAATGAGAAGTTTCGCGGTGTACGCATTCAGTTAGAAGCGGGATTACTGAAAATCACCG
CCAATAACCCTGAGCAGGAAGAAGCGGAAGAAATCATCGATGTGGATTACAACAATCAGCCACTCGAAATTGGTTTCAAC
GTCAGTTACTTATTGGATGTGTTAAACAACCTTAAGTCCGATGATGTGCGTATCACCTTAATCGATGGTAACTCGAGTGC
CTTATTGGAAAACCACCTGGAAGAAGATTCCATGTATGTGGTTATGCCGATGCGCTTATAG

Upstream 100 bases:

>100_bases
GTAAGATTGCTCAGCTACGGGAAGAGAGTCACGACATTAAAGAAGATTATGCTAACTTGATTAGAACCTTATCTTCTTAA
AATCAAGGAATTTGGACACT

Downstream 100 bases:

>100_bases
TATTCCATTGCCTACTTTGTGCCATACGCCGCTTTTTAGCGGCGTATATGTCTTCAAGCCTTTATCCTTTGTTGCCTTAT
TTACTCGGATGGATGTGAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIHGDIEEGRTTVPAKKLLDIVK
SLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVEAFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR
FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQEEAEEIIDVDYNNQPLEIGFN
VSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=57.3770491803279, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40876; Mature: 40876

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEECEEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH
AFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCEEEEEE
HRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR
ECEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCHHEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNNQPLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYV
HHHHHEECCCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVCGAVERRHNLPILANLLVEVSGHSLKMTGTDLEVELVGQAVIH
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCEEEEEECEEEEE
GDIEEGRTTVPAKKLLDIVKSLPEQSELKVEQQDNKWLLRSGRSRFSLATLPAEEYPNVE
CCHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEHHHCCCCCEEEEECCHHHCCCHH
AFQAEIEFTLKQGVLKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCEEEEEE
HRTIDAQLPEKQVIVPRKGVMEMARLLEADDLDIAIAIGDNAIRATTSTTVFTSKLVDGR
ECEECCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCHHEEEHHHHCCC
FPDYRRVLPKGGDKIVIASRNHFKQALTRASILSNEKFRGVRIQLEAGLLKITANNPEQE
CCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHH
EAEEIIDVDYNNQPLEIGFNVSYLLDVLNNLKSDDVRITLIDGNSSALLENHLEEDSMYV
HHHHHEECCCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCEEE
VMPMRL
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]