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The map label for this gene is trmE [H]

Identifier: 117918464

GI number: 117918464

Start: 5376

End: 6737

Strand: Reverse

Name: trmE [H]

Synonym: Shewana3_0005

Alternate gene names: 117918464

Gene position: 6737-5376 (Counterclockwise)

Preceding gene: 117918465

Following gene: 117918462

Centisome position: 0.14

GC content: 50.81

Gene sequence:

>1362_bases
GTGACAACTGACACTATCGTGGCACAGGCCACCGCGCCCGGACGGGGCGGCGTGGGTATCATTCGTATCTCTGGTGATAA
GGCAACCGATGTGGCCATGGCCGTATTGGGTCATTTACCTAAACCCCGTTACGCCGATTACTGCGACTTTAAAAATGCTT
CAGGCCAAGTGATAGACCAAGGTATCGCACTGTTTTTTAAAGGCCCTAATTCCTTCACCGGTGAAGACGTATTAGAGCTG
CAGGGCCACGGTGGTCAAATCGTTCTCGATATGCTGATCAAGCGCGTATTAGAAGTCGAAGGCATTCGCATCGCCAAACC
AGGCGAGTTTAGCGAACAGGCCTTTATGAACGACAAGCTAGACTTAACCCAGGCCGAAGCCATTGCCGACCTTATCGATG
CCACCAGTGAGCAAGCCGCCAAAAGCGCGCTGCAATCACTGCAAGGTGAGTTTTCGAAGGAAGTCCATGAGCTGGTGGAT
CAAGTCACCCATCTGCGTTTATACGTCGAAGCCGCGATTGATTTCCCCGATGAAGAAGTCGACTTTTTAAGTGACGGCAA
AATTGCCAACGCGCTCTACAAAATCATCGATAAACTCAGCGCCGTGCAGGCCAGTGCCAAGCAAGGCTCGATTATCCGCG
AAGGGATGAAAGTGGTCATTGCTGGCAGGCCAAATGCGGGTAAATCGAGTCTGCTTAACGCCCTAGCGGGTAAAGAGTCA
GCGATTGTCACCGAAATTGCTGGCACCACCCGTGACGTATTGCGCGAACATATCCATTTAGATGGTATGCCACTACATAT
TATCGACACTGCAGGTTTACGTGACACCACTGACACGGTTGAACAAATCGGTATCGAACGCGCGTGGAACGAAATCAACA
GCGCCGATCGGGTGTTATTTATGGTCGATGGCACCACCACGGATGCGGTCGATCCCCATGATATTTGGCCCGATTTTATC
AATCGCCTGCCCGCCAATTTAGGCGTGACCGTTGTCCGCAATAAGGCCGATCTCACCGGTGAGAACCTCGCCATGACCGA
AGAAAAAGGTTACAGCGTGTATCGGATCTCGGCCAAAACCGGTCTAGGCGTAGAGGAATTAAAGCAACACCTTAAATCCT
TGATGGGCTATAAGAGTAATCTCGAAGGTGGCTTTATCGCCCGTCGCCGCCACCTCGAAGCATTAGATGTTGCCGCAAGC
CATCTACAACTCGGTAAAGAACAATTAGAGATTTACCTCGCAGGCGAACTGTTAGCGGAAGAACTGAGAATGGCGCAATT
AGCCCTGTCTGAAATCACCGGCCGCTTCACCTCAGACGATCTATTGGGCAAAATTTTCAGCTCATTCTGTATCGGAAAGT
AA

Upstream 100 bases:

>100_bases
CAAGCGCTAGATAAGAGCTAACGGCTCAGCATATGCAAAAAGGCGGCTTAATGAGCCGCCTTTTCCTTTAGATGATTTGA
TCGAATTAATGGGTATTTCC

Downstream 100 bases:

>100_bases
TTTCGCTTTAGCGTTTAGCTAAACGAGCAATAAAAAATCAGCCCACTAAGTTCGCTTAGGGGCTGATTTTTTATTTAAAG
GCGCTTTGTCTTAAATAGTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTDTIVAQATAPGRGGVGIIRISGDKATDVAMAVLGHLPKPRYADYCDFKNASGQVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVLEVEGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVD
QVTHLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLSAVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSADRVLFMVDGTTTDAVDPHDIWPDFI
NRLPANLGVTVVRNKADLTGENLAMTEEKGYSVYRISAKTGLGVEELKQHLKSLMGYKSNLEGGFIARRRHLEALDVAAS
HLQLGKEQLEIYLAGELLAEELRMAQLALSEITGRFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_453_residues
MTTDTIVAQATAPGRGGVGIIRISGDKATDVAMAVLGHLPKPRYADYCDFKNASGQVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVLEVEGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVD
QVTHLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLSAVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSADRVLFMVDGTTTDAVDPHDIWPDFI
NRLPANLGVTVVRNKADLTGENLAMTEEKGYSVYRISAKTGLGVEELKQHLKSLMGYKSNLEGGFIARRRHLEALDVAAS
HLQLGKEQLEIYLAGELLAEELRMAQLALSEITGRFTSDDLLGKIFSSFCIGK
>Mature_452_residues
TTDTIVAQATAPGRGGVGIIRISGDKATDVAMAVLGHLPKPRYADYCDFKNASGQVIDQGIALFFKGPNSFTGEDVLELQ
GHGGQIVLDMLIKRVLEVEGIRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQ
VTHLRLYVEAAIDFPDEEVDFLSDGKIANALYKIIDKLSAVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKESA
IVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSADRVLFMVDGTTTDAVDPHDIWPDFIN
RLPANLGVTVVRNKADLTGENLAMTEEKGYSVYRISAKTGLGVEELKQHLKSLMGYKSNLEGGFIARRRHLEALDVAASH
LQLGKEQLEIYLAGELLAEELRMAQLALSEITGRFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.6808510638298, Blast_Score=225, Evalue=7e-59,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=34.5652173913044, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=32.6, Blast_Score=211, Evalue=9e-55,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=74.2793791574279, Blast_Score=690, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=38.4615384615385, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=472, Percent_Identity=28.8135593220339, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=32.3293172690763, Blast_Score=205, Evalue=9e-54,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=32.4894514767932, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49169; Mature: 49037

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIVAQATAPGRGGVGIIRISGDKATDVAMAVLGHLPKPRYADYCDFKNASGQVIDQ
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVLEVEGIRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQVTHLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
DFLSDGKIANALYKIIDKLSAVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
MVDGTTTDAVDPHDIWPDFINRLPANLGVTVVRNKADLTGENLAMTEEKGYSVYRISAKT
EEECCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCC
GLGVEELKQHLKSLMGYKSNLEGGFIARRRHLEALDVAASHLQLGKEQLEIYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEEEEHHHHHHH
ELRMAQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDTIVAQATAPGRGGVGIIRISGDKATDVAMAVLGHLPKPRYADYCDFKNASGQVIDQ
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVLEVEGIRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALQSLQGEFSKEVHELVDQVTHLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
DFLSDGKIANALYKIIDKLSAVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTTDTVEQIGIERAWNEINSADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
MVDGTTTDAVDPHDIWPDFINRLPANLGVTVVRNKADLTGENLAMTEEKGYSVYRISAKT
EEECCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEECCC
GLGVEELKQHLKSLMGYKSNLEGGFIARRRHLEALDVAASHLQLGKEQLEIYLAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEEEEHHHHHHH
ELRMAQLALSEITGRFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA