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The map label for this gene is rep [H]

Identifier: 117676144

GI number: 117676144

Start: 68453

End: 70252

Strand: Reverse

Name: rep [H]

Synonym: Shewana3_4204

Alternate gene names: 117676144

Gene position: 70252-68453 (Counterclockwise)

Preceding gene: 117676146

Following gene: 117676143

Centisome position: 25.19

GC content: 47.94

Gene sequence:

>1800_bases
ATGGGCTCAACAAAAGATCAGGGGTTAGTCATCGAACGTGACGACAATACCCTTGTTTGTGCATTACCTGGAAGTGGTAA
AACCTATGTTTTGGTAGAGCTGACTAAGCGTTTACTGTCCAGAGATGGCGCCTATTCTGTCCATTTACTTACGTTCACGG
ATGCGGCTCGTGTTGAGCTCACTGAGCGAATGCTTCACGCCCTTACCCCACGAGCTATGGAACGGGTAACTATCAGTACC
TTTCATTCAACTGCGTTAGCGATGACTCGCGGCAAACTTAATCGCCGGTTATTAATGGCCAATGAGTTAATGCTGTTCGT
TAAGTCGGTGCTCAATGAAGCTGACCATTTAGACCTCATTGATAAGTTCAGATTTAAGGTGCCGGACGTTGTTAGGCTGT
TTGATGAAATGGGCCGAAGCCAAGACCTGTCAAAAGAGTATAGTGGCACAGAAGTTGCCTTGTACGAGCTTTACAAAGAT
ATGCTGATTAAGCAGGGCAAGACGGATTTTAACGTCGTATGCCAATTGTCCGTGAGCTGGCTGGCGCAAAGGGAAATCCC
TCCTGTGTCGGCTACGCATATTCTGGTAGACGAATTTCAAGATACCGATGCACTGCAATACATGTGGCTACGTGAACACG
CGCTGCTTGGTAAAAAGCTGGTGGTCGTTGGTGATGACGATCAAGCTATCTACTCGTTTAGGGGTGGCCAAGGATACGAC
AATATGGTGGCCTTTCAGTCAGAGTTTAACGCAGATGCTTACGTGTTAAGAGACTGCTTTAGATGCTCAAAGGAAATCCT
GAATCGAGCGGGGATGCTGATTAAGTTCAATAACCCCGATAGGATTGATAAGCCGATGAATGCGGTTAGGGAAGAGCAAG
GTACTGTCAAAATCATTAAGTCTGGCACGATGGATAACCAGCTTAACCAGATTTTAGATGCGGTGAGCAAAGAGCCTCAT
ACGTGGGCAGTAATTGCGAGGAACAATGCCATCCTTGACCGCGTTGAAACGGTTATTCAGGGTCATGGCATTGAGTACAA
TCGACGCGGCGGTAAGTCGTTTTTTGATGGCGTAGCACCCTACAGCTTGATTCAAATTGCCTCTGTGATTTCAGGCAAAC
ATGATGACCATACCCTGCATCGGGCTTTGGCGGCTTTGTCAGTGCCAGAGGCGGAAACGGCAAGAATTATTGGTTTATGC
CGGACGGCGAAAACGACCTTTGGCGGCCTTAAACATGCGGAGATTGGTCATCATGCTGACCTGCAAAAGTTCCACTCATT
AGCTGAAGCCTGGCATGACATACCTGCAGGGCGTGAGGATGCTAAGCGAACCTACATGCAATCTATCGTTAAATTGTTAG
CCATACACCTCGATAAGCGCGACCAAAAACATGCTCAAGTCGTCGGTGATGTCATTGCTGGACGTCACTGGGAGAGCCTG
GGCGAAAGTATTGCGCAACTGAAGTCTTCGTTAGACTGCAAAAAGCGTAAAGACCAATCAGAAGAGGCCGCACCTAAGCT
GTCATTGATCACTATGCACTCTTCAAAAGGTTTGCAGTTCGACAACGTGTGGATTGTGGGGAGCGACACAGGAACCATCC
CGAGCAAAGAGGCGTTAGCAGAGGGTAAGATAGCGGAAGAACGTCGATTAATGTATGTGGCCATGACCCGCGCTATCTCG
TCGCTCACTATGAGTTACACCAGTGAGCCCAGTGAGTTTCTGTACGAGGTTAACAAAGAAAGGGTTGTAGAGCTGGAGTC
TGGATATGAAGACGATGCTGACGATTTTGACGCCGCTTGA

Upstream 100 bases:

>100_bases
GCAAATTTCTTGAAATATAAGAAGAGTGGTTAAAAGGTTGATTGTTTAGCTTACGAAACTTATCATTCGCGCAACACATA
AATTTAATGGTGAAGTTTAA

Downstream 100 bases:

>100_bases
CATCCTCCACCCCCTAAAGAAGTGTGGGGTTTTACGCTTAAACTGACAGATTGACAAACCAGTGAAATTAAAATAATGTG
TATGTGGGCGGATGCCCTTC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MGSTKDQGLVIERDDNTLVCALPGSGKTYVLVELTKRLLSRDGAYSVHLLTFTDAARVELTERMLHALTPRAMERVTIST
FHSTALAMTRGKLNRRLLMANELMLFVKSVLNEADHLDLIDKFRFKVPDVVRLFDEMGRSQDLSKEYSGTEVALYELYKD
MLIKQGKTDFNVVCQLSVSWLAQREIPPVSATHILVDEFQDTDALQYMWLREHALLGKKLVVVGDDDQAIYSFRGGQGYD
NMVAFQSEFNADAYVLRDCFRCSKEILNRAGMLIKFNNPDRIDKPMNAVREEQGTVKIIKSGTMDNQLNQILDAVSKEPH
TWAVIARNNAILDRVETVIQGHGIEYNRRGGKSFFDGVAPYSLIQIASVISGKHDDHTLHRALAALSVPEAETARIIGLC
RTAKTTFGGLKHAEIGHHADLQKFHSLAEAWHDIPAGREDAKRTYMQSIVKLLAIHLDKRDQKHAQVVGDVIAGRHWESL
GESIAQLKSSLDCKKRKDQSEEAAPKLSLITMHSSKGLQFDNVWIVGSDTGTIPSKEALAEGKIAEERRLMYVAMTRAIS
SLTMSYTSEPSEFLYEVNKERVVELESGYEDDADDFDAA

Sequences:

>Translated_599_residues
MGSTKDQGLVIERDDNTLVCALPGSGKTYVLVELTKRLLSRDGAYSVHLLTFTDAARVELTERMLHALTPRAMERVTIST
FHSTALAMTRGKLNRRLLMANELMLFVKSVLNEADHLDLIDKFRFKVPDVVRLFDEMGRSQDLSKEYSGTEVALYELYKD
MLIKQGKTDFNVVCQLSVSWLAQREIPPVSATHILVDEFQDTDALQYMWLREHALLGKKLVVVGDDDQAIYSFRGGQGYD
NMVAFQSEFNADAYVLRDCFRCSKEILNRAGMLIKFNNPDRIDKPMNAVREEQGTVKIIKSGTMDNQLNQILDAVSKEPH
TWAVIARNNAILDRVETVIQGHGIEYNRRGGKSFFDGVAPYSLIQIASVISGKHDDHTLHRALAALSVPEAETARIIGLC
RTAKTTFGGLKHAEIGHHADLQKFHSLAEAWHDIPAGREDAKRTYMQSIVKLLAIHLDKRDQKHAQVVGDVIAGRHWESL
GESIAQLKSSLDCKKRKDQSEEAAPKLSLITMHSSKGLQFDNVWIVGSDTGTIPSKEALAEGKIAEERRLMYVAMTRAIS
SLTMSYTSEPSEFLYEVNKERVVELESGYEDDADDFDAA
>Mature_598_residues
GSTKDQGLVIERDDNTLVCALPGSGKTYVLVELTKRLLSRDGAYSVHLLTFTDAARVELTERMLHALTPRAMERVTISTF
HSTALAMTRGKLNRRLLMANELMLFVKSVLNEADHLDLIDKFRFKVPDVVRLFDEMGRSQDLSKEYSGTEVALYELYKDM
LIKQGKTDFNVVCQLSVSWLAQREIPPVSATHILVDEFQDTDALQYMWLREHALLGKKLVVVGDDDQAIYSFRGGQGYDN
MVAFQSEFNADAYVLRDCFRCSKEILNRAGMLIKFNNPDRIDKPMNAVREEQGTVKIIKSGTMDNQLNQILDAVSKEPHT
WAVIARNNAILDRVETVIQGHGIEYNRRGGKSFFDGVAPYSLIQIASVISGKHDDHTLHRALAALSVPEAETARIIGLCR
TAKTTFGGLKHAEIGHHADLQKFHSLAEAWHDIPAGREDAKRTYMQSIVKLLAIHLDKRDQKHAQVVGDVIAGRHWESLG
ESIAQLKSSLDCKKRKDQSEEAAPKLSLITMHSSKGLQFDNVWIVGSDTGTIPSKEALAEGKIAEERRLMYVAMTRAISS
LTMSYTSEPSEFLYEVNKERVVELESGYEDDADDFDAA

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=639, Percent_Identity=25.9780907668232, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI48994965, Length=635, Percent_Identity=26.1417322834646, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 67180; Mature: 67049

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSTKDQGLVIERDDNTLVCALPGSGKTYVLVELTKRLLSRDGAYSVHLLTFTDAARVEL
CCCCCCCCEEEEECCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
TERMLHALTPRAMERVTISTFHSTALAMTRGKLNRRLLMANELMLFVKSVLNEADHLDLI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DKFRFKVPDVVRLFDEMGRSQDLSKEYSGTEVALYELYKDMLIKQGKTDFNVVCQLSVSW
HHHHCCCHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHH
LAQREIPPVSATHILVDEFQDTDALQYMWLREHALLGKKLVVVGDDDQAIYSFRGGQGYD
HHHCCCCCCCHHEEEHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC
NMVAFQSEFNADAYVLRDCFRCSKEILNRAGMLIKFNNPDRIDKPMNAVREEQGTVKIIK
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEE
SGTMDNQLNQILDAVSKEPHTWAVIARNNAILDRVETVIQGHGIEYNRRGGKSFFDGVAP
CCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCH
YSLIQIASVISGKHDDHTLHRALAALSVPEAETARIIGLCRTAKTTFGGLKHAEIGHHAD
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LQKFHSLAEAWHDIPAGREDAKRTYMQSIVKLLAIHLDKRDQKHAQVVGDVIAGRHWESL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
GESIAQLKSSLDCKKRKDQSEEAAPKLSLITMHSSKGLQFDNVWIVGSDTGTIPSKEALA
HHHHHHHHHHCCHHHHCCCCHHHCCCEEEEEEECCCCCEECCEEEEECCCCCCCCHHHHH
EGKIAEERRLMYVAMTRAISSLTMSYTSEPSEFLYEVNKERVVELESGYEDDADDFDAA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GSTKDQGLVIERDDNTLVCALPGSGKTYVLVELTKRLLSRDGAYSVHLLTFTDAARVEL
CCCCCCCEEEEECCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
TERMLHALTPRAMERVTISTFHSTALAMTRGKLNRRLLMANELMLFVKSVLNEADHLDLI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DKFRFKVPDVVRLFDEMGRSQDLSKEYSGTEVALYELYKDMLIKQGKTDFNVVCQLSVSW
HHHHCCCHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHH
LAQREIPPVSATHILVDEFQDTDALQYMWLREHALLGKKLVVVGDDDQAIYSFRGGQGYD
HHHCCCCCCCHHEEEHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC
NMVAFQSEFNADAYVLRDCFRCSKEILNRAGMLIKFNNPDRIDKPMNAVREEQGTVKIIK
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEE
SGTMDNQLNQILDAVSKEPHTWAVIARNNAILDRVETVIQGHGIEYNRRGGKSFFDGVAP
CCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCH
YSLIQIASVISGKHDDHTLHRALAALSVPEAETARIIGLCRTAKTTFGGLKHAEIGHHAD
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LQKFHSLAEAWHDIPAGREDAKRTYMQSIVKLLAIHLDKRDQKHAQVVGDVIAGRHWESL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
GESIAQLKSSLDCKKRKDQSEEAAPKLSLITMHSSKGLQFDNVWIVGSDTGTIPSKEALA
HHHHHHHHHHCCHHHHCCCCHHHCCCEEEEEEECCCCCEECCEEEEECCCCCCCCHHHHH
EGKIAEERRLMYVAMTRAISSLTMSYTSEPSEFLYEVNKERVVELESGYEDDADDFDAA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438 [H]