| Definition | Shewanella sp. ANA-3 plasmid 1, complete sequence. |
|---|---|
| Accession | NC_008573 |
| Length | 278,942 |
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The map label for this gene is scrFIAM [H]
Identifier: 117676134
GI number: 117676134
Start: 56546
End: 57919
Strand: Reverse
Name: scrFIAM [H]
Synonym: Shewana3_4194
Alternate gene names: 117676134
Gene position: 57919-56546 (Counterclockwise)
Preceding gene: 117676135
Following gene: 117676133
Centisome position: 20.76
GC content: 47.89
Gene sequence:
>1374_bases ATGGCAACAATCATTAATACTAAAATTGGTGATTCCAAAGGCCGCGCTCGTGTGTGGGTCGAGGGTTCCAAGCTATCACG AGAAGGGTGGGATGCAGGTCGAAATTACACTATTCACGCAAACGGAAATGAAATCCGCTTAGCGGCAGATGAACAGGGTA AGTACACCGTAAGCCGTCGCAGTAAAAACGGTAATATCACACCCATTATCGATTTAACCTGTAAAGCGATTACAGAGGTT TTTGAAGGCATTAGCAAACTCAGAGTGTTGATTAAAGCAGGGGTAATCATTATCTCAGCGCATCATCAATCTGAGCGGGT TAAGAGTCGGGAATTACGATTAATGTCACGCCTGCAAAATGGTGAGTCATTGAAAGTGGCCTCGTTATTCCATGGCGGTG GCGTGTTAGATAAGGCTGTCCATCATGGATTAGAGTCGGCGGGGATCTCCAGCAAAATAGCTGTCGCTGTTGAGCTAGAG TCCCAGTACCTAGATTCGAGCCTGACCAACAATCCAGAGTTGTGGGATAGCAGTTCTATTGTGATTGAGTCTCCGGTTCA AGATGTGAACTTGCAGCAATGCAATATGGAGGTCGATGTGCTGGTTGCGGGTATCCCTTGCGTCGGCGCATCAAAAAGCG GTCGAAGTAAACTGAAGCTCCAGTTCGCAGAATCTCACGATTCGGCAGGCGCTTTGTTCTTCTCGTTTCTTCAGTTCGCG CAAGTGTTAAATCCGGCGATTATCTTGATAGAAAATGTGGTCGAATACGCCAACACTTCATCAATGACAGTCATCAGGTC TGTGCTGGGTTCGTTGGGGTACAACCTCCACGAAACCACATTAGATGGTGGCCGCTGGGTTTTAGAACGTCGTAAACGCT TAGCCGTTGTCGCTCTGTCGTCGGGAATTGATATGTCAGAGTTTGACATCTATTCACTTATGCCTTCTCGCTTTCCAGAG GCCAGTCTAGGGCATATTTTGGATGCGGTGCCGGATGGCGACGACAGGTACAAGCCGTTTGATTATCTGGCGGCGAAGGA AGAGCGCGATAAAGCGGCAGGTAAGGGGTTTGCTCGTCAGTTATTGACAGCAGATTCCGATGGCTGTGGTGTGATAGGCA AGGACTACGCGAAATGCCGTAGCACTGAGCCATTCATCATCAATCCAGTTGACTCCGCGTTATCAAGATTGTTCACACCG GTTGAGCATTGCCGCGTGAAGGGAATCCCAGTTGAAGTCATTCAGGGATTATCTGACACAGTGGCGCATCAAATCCTTGG GCAATCGGTGATATACCCTGCCTTTAAAGACTTGATGCAATCAGTAGGACAAACGCTTTTAACCAATACCGTTAAAGCTG CCATTGCAGCTTAA
Upstream 100 bases:
>100_bases TTAACATCAAAATTTAACCCTTGGGGTGAGTAATGTCCCCTAGGGCCATAGCTCACCCTTTTTTGTTTTATGCCTTACGG GCATTTATGGAGTGAGTCAC
Downstream 100 bases:
>100_bases CATCAAAATTTAACCCTTGGGGGGGGGTGCCATGCCCCCAAGGGCCATGCTCACCCCTTTTTTATACCTGCCAACGGGCA ATAGGAGTGAGTTATGGGAT
Product: C-5 cytosine-specific DNA methylase
Products: NA
Alternate protein names: M.ScrFI-A; M.ScrFIA; Cytosine-specific methyltransferase ScrFIA [H]
Number of amino acids: Translated: 457; Mature: 456
Protein sequence:
>457_residues MATIINTKIGDSKGRARVWVEGSKLSREGWDAGRNYTIHANGNEIRLAADEQGKYTVSRRSKNGNITPIIDLTCKAITEV FEGISKLRVLIKAGVIIISAHHQSERVKSRELRLMSRLQNGESLKVASLFHGGGVLDKAVHHGLESAGISSKIAVAVELE SQYLDSSLTNNPELWDSSSIVIESPVQDVNLQQCNMEVDVLVAGIPCVGASKSGRSKLKLQFAESHDSAGALFFSFLQFA QVLNPAIILIENVVEYANTSSMTVIRSVLGSLGYNLHETTLDGGRWVLERRKRLAVVALSSGIDMSEFDIYSLMPSRFPE ASLGHILDAVPDGDDRYKPFDYLAAKEERDKAAGKGFARQLLTADSDGCGVIGKDYAKCRSTEPFIINPVDSALSRLFTP VEHCRVKGIPVEVIQGLSDTVAHQILGQSVIYPAFKDLMQSVGQTLLTNTVKAAIAA
Sequences:
>Translated_457_residues MATIINTKIGDSKGRARVWVEGSKLSREGWDAGRNYTIHANGNEIRLAADEQGKYTVSRRSKNGNITPIIDLTCKAITEV FEGISKLRVLIKAGVIIISAHHQSERVKSRELRLMSRLQNGESLKVASLFHGGGVLDKAVHHGLESAGISSKIAVAVELE SQYLDSSLTNNPELWDSSSIVIESPVQDVNLQQCNMEVDVLVAGIPCVGASKSGRSKLKLQFAESHDSAGALFFSFLQFA QVLNPAIILIENVVEYANTSSMTVIRSVLGSLGYNLHETTLDGGRWVLERRKRLAVVALSSGIDMSEFDIYSLMPSRFPE ASLGHILDAVPDGDDRYKPFDYLAAKEERDKAAGKGFARQLLTADSDGCGVIGKDYAKCRSTEPFIINPVDSALSRLFTP VEHCRVKGIPVEVIQGLSDTVAHQILGQSVIYPAFKDLMQSVGQTLLTNTVKAAIAA >Mature_456_residues ATIINTKIGDSKGRARVWVEGSKLSREGWDAGRNYTIHANGNEIRLAADEQGKYTVSRRSKNGNITPIIDLTCKAITEVF EGISKLRVLIKAGVIIISAHHQSERVKSRELRLMSRLQNGESLKVASLFHGGGVLDKAVHHGLESAGISSKIAVAVELES QYLDSSLTNNPELWDSSSIVIESPVQDVNLQQCNMEVDVLVAGIPCVGASKSGRSKLKLQFAESHDSAGALFFSFLQFAQ VLNPAIILIENVVEYANTSSMTVIRSVLGSLGYNLHETTLDGGRWVLERRKRLAVVALSSGIDMSEFDIYSLMPSRFPEA SLGHILDAVPDGDDRYKPFDYLAAKEERDKAAGKGFARQLLTADSDGCGVIGKDYAKCRSTEPFIINPVDSALSRLFTPV EHCRVKGIPVEVIQGLSDTVAHQILGQSVIYPAFKDLMQSVGQTLLTNTVKAAIAA
Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]
COG id: COG0270
COG function: function code L; Site-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018117 - InterPro: IPR001525 - InterPro: IPR001387 - InterPro: IPR010982 [H]
Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]
EC number: =2.1.1.37 [H]
Molecular weight: Translated: 49653; Mature: 49522
Theoretical pI: Translated: 7.09; Mature: 7.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MATIINTKIGDSKGRARVWVEGSKLSREGWDAGRNYTIHANGNEIRLAADEQGKYTVSRR CCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEE SKNGNITPIIDLTCKAITEVFEGISKLRVLIKAGVIIISAHHQSERVKSRELRLMSRLQN CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCC GESLKVASLFHGGGVLDKAVHHGLESAGISSKIAVAVELESQYLDSSLTNNPELWDSSSI CCCEEEEEEECCCCHHHHHHHCCHHHCCCCCEEEEEEEECHHHHHHHCCCCCCCCCCCCE VIESPVQDVNLQQCNMEVDVLVAGIPCVGASKSGRSKLKLQFAESHDSAGALFFSFLQFA EEECCCCCCCHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH QVLNPAIILIENVVEYANTSSMTVIRSVLGSLGYNLHETTLDGGRWVLERRKRLAVVALS HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHEEEEEEEC SGIDMSEFDIYSLMPSRFPEASLGHILDAVPDGDDRYKPFDYLAAKEERDKAAGKGFARQ CCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH LLTADSDGCGVIGKDYAKCRSTEPFIINPVDSALSRLFTPVEHCRVKGIPVEVIQGLSDT HHHCCCCCCCCCCCCHHHHCCCCCEEECCHHHHHHHHHCCHHHHEECCCHHHHHHCHHHH VAHQILGQSVIYPAFKDLMQSVGQTLLTNTVKAAIAA HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure ATIINTKIGDSKGRARVWVEGSKLSREGWDAGRNYTIHANGNEIRLAADEQGKYTVSRR CEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEE SKNGNITPIIDLTCKAITEVFEGISKLRVLIKAGVIIISAHHQSERVKSRELRLMSRLQN CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCC GESLKVASLFHGGGVLDKAVHHGLESAGISSKIAVAVELESQYLDSSLTNNPELWDSSSI CCCEEEEEEECCCCHHHHHHHCCHHHCCCCCEEEEEEEECHHHHHHHCCCCCCCCCCCCE VIESPVQDVNLQQCNMEVDVLVAGIPCVGASKSGRSKLKLQFAESHDSAGALFFSFLQFA EEECCCCCCCHHHCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH QVLNPAIILIENVVEYANTSSMTVIRSVLGSLGYNLHETTLDGGRWVLERRKRLAVVALS HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHEEEEEEEC SGIDMSEFDIYSLMPSRFPEASLGHILDAVPDGDDRYKPFDYLAAKEERDKAAGKGFARQ CCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH LLTADSDGCGVIGKDYAKCRSTEPFIINPVDSALSRLFTPVEHCRVKGIPVEVIQGLSDT HHHCCCCCCCCCCCCHHHHCCCCCEEECCHHHHHHHHHCCHHHHEECCCHHHHHHCHHHH VAHQILGQSVIYPAFKDLMQSVGQTLLTNTVKAAIAA HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8481004 [H]