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The map label for this gene is yclM [H]

Identifier: 116873739

GI number: 116873739

Start: 2369360

End: 2370721

Strand: Direct

Name: yclM [H]

Synonym: lwe2323

Alternate gene names: 116873739

Gene position: 2369360-2370721 (Clockwise)

Preceding gene: 116873737

Following gene: 116873742

Centisome position: 84.2

GC content: 39.35

Gene sequence:

>1362_bases
ATGAAAGTAATTAAATTTGGCGGAAGCTCTTTAGCATCCGGGATTCAATTAAATAAGGTTTTCCAACTTGTAGCGGAGGA
TTCTGACCGAAAAATCGTCGTAGTTTCTGCACCTGGCAAGCGTTTTAAAGCAGATACAAAAGTGACTGATTTACTTATTG
ATTGCGCAACAAAGGCACTTCTAGGAGAAGATACAAGTGAATTATTTGAAGCAGTTATTGCCAGATATGCTGGTATTGCA
CTTGATACAGGAATGGACGACACCATCATTAAACAAATTCGCTCGGACTTACAGGCGACCATTTCATCTGATAAAAGCGA
CCCCGATAAATTTTTAGATCGAATGAAAGCTAGTGGGGAAGATAATAATGCGAAGTTAATCGCGGCTTATTTTAAATTCA
AAGGCTTAAATGCCAATTATATTAATCCAAAAGATGCTGGTTTACTTGTGACAGATGAACATGCAAGCGCGCAAGTTTTA
CCTGAGTCATATGACCGTTTATTTGCACTTCGAGAACGAGAAGGAATTATTGTTTTCCCAGGATTTTTTGGTTATACAAA
AGACGGCGAAATTAGCACATTTTCTCGAAGTGGATCTGATATTACCGGAGCAATCGTGGCGAATGGTGCGCAAGCTGAGT
TATATGAGAACTTCACTGATGTTGATGCTGTATACGCGGTCAATCCCGCTATTGTGAAAAATCCAAAAAAAGTGCTCGAG
CTTACTTACCGAGAAATGCGTGAACTTTCTTACGCTGGCTTCTCTGTTTTTCACGATGAAGCTTTAATTCCAGCGTTCCA
TGCGGGCATCCCCGTACATATAAAAAATACGAATAATCCTGATTCTTGTGGTACGCGTGTTGTGCATGAACGGGAAAATA
ATAATGGTCCTGTGGTTGGAATTGCAAGTGATGACGGCTTTTGTAGCATTTATATAAGTAAATATTTGATGAACCGCGAA
ATCGGCTTCGGACGAAAAGTATTGCAAATTTTGGAAGATGCTGGGTTGAACTATGAACATATGCCATCCGGAATTGATGA
TTTAACGATTATTATTCGTGAAAACCAGTTTAGTGAGCATACGGAGCAGACGATTATGTCTCGTTTGAAAGAAGAATTGA
ATGCCGACCAAGTGATTATGCAACACGGCATTTCGCTAATTATGGTGGTTGGCGAAACAATGCGTCATAATGTTGGAATA
ACATCACGCGCTTCCAAAGCTTTATCGGACGCAAAAGTGAATATAGAAATGATTAATCAAGGTTCTTCTGAAGTGAGTAT
TATGTTCGGTGTGAAAGAAGAACAAGAAAACACAGCGGTTCGAGCGTTGTATGATGAATTCTTTTCGGAAGTTTTCGTTT
AA

Upstream 100 bases:

>100_bases
ATAATAGACAATAACTTTATCCGAAAGCTCAGAAAATTTCGCATCATTCAAAAACAACACTTTCGGGGTGAAACAAAAAT
TTAAATCAGGAGTGTGTAGC

Downstream 100 bases:

>100_bases
AAATAAAACAAGTTTTTCATGTTGGTTCATGAAAAACTTGTTTTTTGTTTTATTTAATATTAATCTTCTCAATCACAACG
TCATTCACTGGTTTGTCTTG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDSDRKIVVVSAPGKRFKADTKVTDLLIDCATKALLGEDTSELFEAVIARYAGIA
LDTGMDDTIIKQIRSDLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFSEHTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYDEFFSEVFV

Sequences:

>Translated_453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDSDRKIVVVSAPGKRFKADTKVTDLLIDCATKALLGEDTSELFEAVIARYAGIA
LDTGMDDTIIKQIRSDLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFSEHTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYDEFFSEVFV
>Mature_453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDSDRKIVVVSAPGKRFKADTKVTDLLIDCATKALLGEDTSELFEAVIARYAGIA
LDTGMDDTIIKQIRSDLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFSEHTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYDEFFSEVFV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=470, Percent_Identity=27.6595744680851, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790455, Length=464, Percent_Identity=23.4913793103448, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790376, Length=316, Percent_Identity=25, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=375, Percent_Identity=21.8666666666667, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49956; Mature: 49956

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKFGGSSLASGIQLNKVFQLVAEDSDRKIVVVSAPGKRFKADTKVTDLLIDCATKAL
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LGEDTSELFEAVIARYAGIALDTGMDDTIIKQIRSDLQATISSDKSDPDKFLDRMKASGE
HCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVLPESYDRLFALREREGIIVFP
CCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
GFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
CCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCEEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVG
HHHHHHHHHHHCCHHHCCCCCCCHHHHCCCEEEECCCCCCCHHCCCEEEECCCCCCCEEE
IASDDGFCSIYISKYLMNREIGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFSEH
EECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCHHH
TEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGITSRASKALSDAKVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHCCEEEEEEECC
GSSEVSIMFGVKEEQENTAVRALYDEFFSEVFV
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVIKFGGSSLASGIQLNKVFQLVAEDSDRKIVVVSAPGKRFKADTKVTDLLIDCATKAL
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LGEDTSELFEAVIARYAGIALDTGMDDTIIKQIRSDLQATISSDKSDPDKFLDRMKASGE
HCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVLPESYDRLFALREREGIIVFP
CCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
GFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
CCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCEEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVG
HHHHHHHHHHHCCHHHCCCCCCCHHHHCCCEEEECCCCCCCHHCCCEEEECCCCCCCEEE
IASDDGFCSIYISKYLMNREIGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFSEH
EECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCHHH
TEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGITSRASKALSDAKVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHCCEEEEEEECC
GSSEVSIMFGVKEEQENTAVRALYDEFFSEVFV
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]