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The map label for this gene is malS [H]

Identifier: 116873350

GI number: 116873350

Start: 1963773

End: 1965416

Strand: Direct

Name: malS [H]

Synonym: lwe1934

Alternate gene names: 116873350

Gene position: 1963773-1965416 (Clockwise)

Preceding gene: 116873349

Following gene: 116873351

Centisome position: 69.78

GC content: 39.72

Gene sequence:

>1644_bases
ATGACTTTTAAACCCGGCTTTGACATCATGAATAATCCACTACTAAACAAAGGAACAGCCTTTACCAAAGAAGAAAGATC
TAACTACAATTTAGACGGATTACTGCCACCAATCATTGAAACAATGGAGCAACAAGCTATCCGTATTGAAAATCAAATTA
AAAATTTAGAAACCGCACTACATAAGCATCAACTATTAACAAATTTATATAACGAAAATCGTACACTTTATTATTATGTT
GTTTCCAAAAATGTTACGGAATATTTGCCCCTTATTTATACTCCAACTATTGGGGATGCCGTAATAAACTATCACAAAGA
ATATTCCGCACCTGATGAAGCTTTATTTATTGATGCCTTTGCTCCTGAAAAATTAAAAACCTCTATTGAAAACTATGCAA
AAAATAATCCAGCTATCGATATGATTGTTATTACTGATGGGGAAGGCGTTCTCGGTATTGGAGACTGGGGTGTAAATGGC
GTCAAAATCGCTGTTGGAAAATTAGCTGTTTATACCGTTGCTGCTGGGCTTGCTCCCGACCGCGTTCTTCCAGTGGTGAT
TGATGCTGGTACAAATAATAAGTCATTACTTGATGATCCGCTTTATTTAGGCAATAAACGACCACGCCTTTCAGAAAAGG
AATATGATCTTTTTATTGCTTCATTCGTTGCAATCATGAATGAAGTCTTTCCAAAAGCGATTCTTCACTGGGAAGATTTT
GGAAGAGCAAATGCGAGCCGTATTCTTCAACATTATCGCGATAAAATTTGCACTTTTAATGATGATATTCAAGGAACTGG
TGCAATGGTAGTAGCAGCAGCACTTGCCACAATTCACGTTTCTCATATTCCTTTAAACAAACAAAAGATTATTATTTTTG
GCGCTGGGACTGCTGGAATTGGAATTGCTGATCAACTTAGCGCACAATTAATGCGCGAAACCGGTCTTCCTTTTGATACT
GCAAAAAAACATTTTTATTTAGTAGATCGAAATGGATTAATACTTGATAATATGACGGATTTAACTACTGGTCAAAAGAA
ATATGCGCAACAAGCTTCTGAATGGGCTAGCATGCCTGCTGGTACTTTAGAAGAGTTAGTTGAAGCAATCCACCCAACAA
TGCTGATTGGCTGTTCTGGCGTTACTGGTGCGTTTAAGGAGAGCATTGTCAGAAAAATGAGCGACCACACTGAACGTCCA
GCTATTTTGCCGCTTTCTAATCCAACTAAATTAGCCGAAGCAACTGCAGCAGATTTAATCAACTGGACAGACGGTAAAGC
TCTTATCGTAACGGGCAGCCCATCTGAACCAGTAGAATATCAAAACACGACGTATGAAATTGGGCAAGCAAATAATGCGC
TTCTTTATCCAGGTTTAGGGCTTGGCGCGCTTGTTACACGTGCCAAATTTATTACTGATGGGATGCTAGCAGCGGCTTCG
AAAGCAGTTGCTGATCAAATTTCACCGAGCGAACCAGGAGCAGCTCTTTTACCCCACGTGCGTACACTTAGGGAGACTTC
TCAGGCCGTGGCTATTGCTGTAGCTAACGAAGCGGTAAAAGAAAATATCCATCAAATCGAATTAACAAACATCCGTGAAG
CTGTCGAACGAGAGATGTGGCACCCCACTTACAAAGGAGTTTAG

Upstream 100 bases:

>100_bases
AAAATCTGATTTATTTTCAGGTTTTCTTTTTATTTGGAATCAGTTGTTATTCTCACTTCCCCCATGTAAAATAGAATAGA
ATGAAAAAGGAGTGTTGAAA

Downstream 100 bases:

>100_bases
TTATGTTCTATAAAACAAAGCACGCGCTTGATAGGCTTGTTCAAAACGGTTCTACCCCAGGAATTAGTTATCAAATTAAA
ACAAAAGATACAGAAGAAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTFKPGFDIMNNPLLNKGTAFTKEERSNYNLDGLLPPIIETMEQQAIRIENQIKNLETALHKHQLLTNLYNENRTLYYYV
VSKNVTEYLPLIYTPTIGDAVINYHKEYSAPDEALFIDAFAPEKLKTSIENYAKNNPAIDMIVITDGEGVLGIGDWGVNG
VKIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNKSLLDDPLYLGNKRPRLSEKEYDLFIASFVAIMNEVFPKAILHWEDF
GRANASRILQHYRDKICTFNDDIQGTGAMVVAAALATIHVSHIPLNKQKIIIFGAGTAGIGIADQLSAQLMRETGLPFDT
AKKHFYLVDRNGLILDNMTDLTTGQKKYAQQASEWASMPAGTLEELVEAIHPTMLIGCSGVTGAFKESIVRKMSDHTERP
AILPLSNPTKLAEATAADLINWTDGKALIVTGSPSEPVEYQNTTYEIGQANNALLYPGLGLGALVTRAKFITDGMLAAAS
KAVADQISPSEPGAALLPHVRTLRETSQAVAIAVANEAVKENIHQIELTNIREAVEREMWHPTYKGV

Sequences:

>Translated_547_residues
MTFKPGFDIMNNPLLNKGTAFTKEERSNYNLDGLLPPIIETMEQQAIRIENQIKNLETALHKHQLLTNLYNENRTLYYYV
VSKNVTEYLPLIYTPTIGDAVINYHKEYSAPDEALFIDAFAPEKLKTSIENYAKNNPAIDMIVITDGEGVLGIGDWGVNG
VKIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNKSLLDDPLYLGNKRPRLSEKEYDLFIASFVAIMNEVFPKAILHWEDF
GRANASRILQHYRDKICTFNDDIQGTGAMVVAAALATIHVSHIPLNKQKIIIFGAGTAGIGIADQLSAQLMRETGLPFDT
AKKHFYLVDRNGLILDNMTDLTTGQKKYAQQASEWASMPAGTLEELVEAIHPTMLIGCSGVTGAFKESIVRKMSDHTERP
AILPLSNPTKLAEATAADLINWTDGKALIVTGSPSEPVEYQNTTYEIGQANNALLYPGLGLGALVTRAKFITDGMLAAAS
KAVADQISPSEPGAALLPHVRTLRETSQAVAIAVANEAVKENIHQIELTNIREAVEREMWHPTYKGV
>Mature_546_residues
TFKPGFDIMNNPLLNKGTAFTKEERSNYNLDGLLPPIIETMEQQAIRIENQIKNLETALHKHQLLTNLYNENRTLYYYVV
SKNVTEYLPLIYTPTIGDAVINYHKEYSAPDEALFIDAFAPEKLKTSIENYAKNNPAIDMIVITDGEGVLGIGDWGVNGV
KIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNKSLLDDPLYLGNKRPRLSEKEYDLFIASFVAIMNEVFPKAILHWEDFG
RANASRILQHYRDKICTFNDDIQGTGAMVVAAALATIHVSHIPLNKQKIIIFGAGTAGIGIADQLSAQLMRETGLPFDTA
KKHFYLVDRNGLILDNMTDLTTGQKKYAQQASEWASMPAGTLEELVEAIHPTMLIGCSGVTGAFKESIVRKMSDHTERPA
ILPLSNPTKLAEATAADLINWTDGKALIVTGSPSEPVEYQNTTYEIGQANNALLYPGLGLGALVTRAKFITDGMLAAASK
AVADQISPSEPGAALLPHVRTLRETSQAVAIAVANEAVKENIHQIELTNIREAVEREMWHPTYKGV

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=552, Percent_Identity=36.9565217391304, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI62420882, Length=523, Percent_Identity=37.4760994263862, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI62420880, Length=523, Percent_Identity=37.4760994263862, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI239049447, Length=523, Percent_Identity=37.4760994263862, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI4505145, Length=513, Percent_Identity=37.4269005847953, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI270265879, Length=456, Percent_Identity=38.8157894736842, Blast_Score=315, Evalue=8e-86,
Organism=Escherichia coli, GI87081919, Length=544, Percent_Identity=40.2573529411765, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17537199, Length=527, Percent_Identity=38.3301707779886, Blast_Score=340, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6322823, Length=563, Percent_Identity=41.563055062167, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21356279, Length=546, Percent_Identity=36.2637362637363, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI281362674, Length=546, Percent_Identity=36.2637362637363, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI281362672, Length=546, Percent_Identity=36.2637362637363, Blast_Score=354, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24646388, Length=527, Percent_Identity=35.8633776091082, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24646386, Length=523, Percent_Identity=36.1376673040153, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI78707242, Length=534, Percent_Identity=33.8951310861423, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI78707238, Length=534, Percent_Identity=33.8951310861423, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI78707240, Length=521, Percent_Identity=34.9328214971209, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI281363505, Length=547, Percent_Identity=31.2614259597806, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI78707236, Length=547, Percent_Identity=31.2614259597806, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI78707232, Length=514, Percent_Identity=32.295719844358, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI281363503, Length=533, Percent_Identity=31.3320825515947, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI19922384, Length=544, Percent_Identity=26.1029411764706, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 59914; Mature: 59783

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKPGFDIMNNPLLNKGTAFTKEERSNYNLDGLLPPIIETMEQQAIRIENQIKNLETAL
CCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HKHQLLTNLYNENRTLYYYVVSKNVTEYLPLIYTPTIGDAVINYHKEYSAPDEALFIDAF
HHHHHHHHHHCCCCEEEEEEECCCHHHHCCEEECCCHHHHHHHHHHHCCCCCCEEEEECC
APEKLKTSIENYAKNNPAIDMIVITDGEGVLGIGDWGVNGVKIAVGKLAVYTVAAGLAPD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEEHHHHHCCCCC
RVLPVVIDAGTNNKSLLDDPLYLGNKRPRLSEKEYDLFIASFVAIMNEVFPKAILHWEDF
CEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRANASRILQHYRDKICTFNDDIQGTGAMVVAAALATIHVSHIPLNKQKIIIFGAGTAGI
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCC
GIADQLSAQLMRETGLPFDTAKKHFYLVDRNGLILDNMTDLTTGQKKYAQQASEWASMPA
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCHHHCCCHHHHHHHHHHHHCCCC
GTLEELVEAIHPTMLIGCSGVTGAFKESIVRKMSDHTERPAILPLSNPTKLAEATAADLI
HHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHE
NWTDGKALIVTGSPSEPVEYQNTTYEIGQANNALLYPGLGLGALVTRAKFITDGMLAAAS
EECCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
KAVADQISPSEPGAALLPHVRTLRETSQAVAIAVANEAVKENIHQIELTNIREAVEREMW
HHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHCCHHEEEHHHHHHHHHHHHC
HPTYKGV
CCCCCCC
>Mature Secondary Structure 
TFKPGFDIMNNPLLNKGTAFTKEERSNYNLDGLLPPIIETMEQQAIRIENQIKNLETAL
CCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HKHQLLTNLYNENRTLYYYVVSKNVTEYLPLIYTPTIGDAVINYHKEYSAPDEALFIDAF
HHHHHHHHHHCCCCEEEEEEECCCHHHHCCEEECCCHHHHHHHHHHHCCCCCCEEEEECC
APEKLKTSIENYAKNNPAIDMIVITDGEGVLGIGDWGVNGVKIAVGKLAVYTVAAGLAPD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEEHHHHHCCCCC
RVLPVVIDAGTNNKSLLDDPLYLGNKRPRLSEKEYDLFIASFVAIMNEVFPKAILHWEDF
CEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRANASRILQHYRDKICTFNDDIQGTGAMVVAAALATIHVSHIPLNKQKIIIFGAGTAGI
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCC
GIADQLSAQLMRETGLPFDTAKKHFYLVDRNGLILDNMTDLTTGQKKYAQQASEWASMPA
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCHHHCCCHHHHHHHHHHHHCCCC
GTLEELVEAIHPTMLIGCSGVTGAFKESIVRKMSDHTERPAILPLSNPTKLAEATAADLI
HHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHE
NWTDGKALIVTGSPSEPVEYQNTTYEIGQANNALLYPGLGLGALVTRAKFITDGMLAAAS
EECCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
KAVADQISPSEPGAALLPHVRTLRETSQAVAIAVANEAVKENIHQIELTNIREAVEREMW
HHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHCCHHEEEHHHHHHHHHHHHC
HPTYKGV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]