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The map label for this gene is ycdT [H]

Identifier: 116873347

GI number: 116873347

Start: 1960830

End: 1961957

Strand: Direct

Name: ycdT [H]

Synonym: lwe1931

Alternate gene names: 116873347

Gene position: 1960830-1961957 (Clockwise)

Preceding gene: 116873346

Following gene: 116873348

Centisome position: 69.68

GC content: 35.64

Gene sequence:

>1128_bases
TTGTTTGAACTAGTTAATTCATACATAGACAGTTTAGCACTCTTTTTAGCTATCCTTTTTATTCAAGGGATGTCTTTTCG
TAAAATAAGACAATATCGCCCAAATTGGTTTCAAAATGGTGGTAGAAGATTGTTTCTTTCAATTCTTCTAGGTATTTATT
ATGGTTTGGCTGGTATTTATTTCATTTATGAAGGAGCTTACGAAAACAACCCTATCATTTATACGAATATGCGAATTTTA
ATTTTAATGGTTACAACGGTTTTCGGTGGTCGTATTCCACTTGTTGTTACTTATCTAATCATGCTTTTTGGAAGAATTAG
CTTTGATGTGGCTTCCCCCGTTACTAGTCGTTATGTTATTTTAATGACATTAATTTTTGCTGCTTGTTTACTCGTTTCTT
ATTGGAAAAAACAGCGCTTCTCTAAATTTATCGCTTTAATCATTTTAAATTTTCCAGCGATACTTTATTATTTCGTAAAC
AATTTCGAGCAAGAGCGAATTTTACGTGGCTTTGAAATCATCGAGTACTTCCTACTTTTCTTCGTAACAGCTTTACTCGT
ATTTTATGCATGTAATTATATCGATAAAAGTAATCTCGTTATCCAAAATTTAACCGAAACCGCGATGACAGATAGCTTAA
CTAATTTACCAAATATGCGTTTTTTTACCCAACAATTCGGATGGATTTTTAATAAAGCGCAGAAAAGAAAAAAAGCACTC
TCCCTCTTCATTATTGATATTGATCATTTTAAAGAAATTAATGATTTTCATGGTCACCAAGCTGGAAACACTGTCTTAGC
GCAGTTCAGCACGATCCTTAAAAATCGCACATTTCCTCCTAGAACGATGTTTGCGCGCATTGGTGGCGAAGAGTTCGCTG
TCTTACTTCAAAATGTCGGGACCGAGCAAGCGGCCTTGATTGCAGATTTTTTGCGGGAAGAAATTGAACGAGCTAAATTC
CCCTATAATCCAGCAAGCGGAAATGTCACTGTATCGATTGGTGTAGCTGCTACAAATGGCAATTTTGCAACTACCGAATC
ACTTTTTGAAGCTGCTGACCAAGCACTCTATCAAGCAAAACAAAATGGACGTAACCAAATCGCCATTCATCAGGGGGAAA
ACAAATGA

Upstream 100 bases:

>100_bases
GATCAAGCCCTTTATAAAGCAAAACGGAATGGGCGAAATCAAGTACACATTCGTCAAACAGAGCCAGTTATTTAAGAGAG
TAAAAAGGAGATGAGTCCGT

Downstream 100 bases:

>100_bases
AACGAATCGTCCTTATTATGATTCCAATATTGCTGATTGCTCTTGGACTTTATTTTCTCTTTCGACCTGAAGTGAAGGAA
ACTTCAAGTGCCCCAAAAGA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGIYYGLAGIYFIYEGAYENNPIIYTNMRIL
ILMVTTVFGGRIPLVVTYLIMLFGRISFDVASPVTSRYVILMTLIFAACLLVSYWKKQRFSKFIALIILNFPAILYYFVN
NFEQERILRGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFNKAQKRKKAL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSTILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFLREEIERAKF
PYNPASGNVTVSIGVAATNGNFATTESLFEAADQALYQAKQNGRNQIAIHQGENK

Sequences:

>Translated_375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGIYYGLAGIYFIYEGAYENNPIIYTNMRIL
ILMVTTVFGGRIPLVVTYLIMLFGRISFDVASPVTSRYVILMTLIFAACLLVSYWKKQRFSKFIALIILNFPAILYYFVN
NFEQERILRGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFNKAQKRKKAL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSTILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFLREEIERAKF
PYNPASGNVTVSIGVAATNGNFATTESLFEAADQALYQAKQNGRNQIAIHQGENK
>Mature_375_residues
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGIYYGLAGIYFIYEGAYENNPIIYTNMRIL
ILMVTTVFGGRIPLVVTYLIMLFGRISFDVASPVTSRYVILMTLIFAACLLVSYWKKQRFSKFIALIILNFPAILYYFVN
NFEQERILRGFEIIEYFLLFFVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFNKAQKRKKAL
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSTILKNRTFPPRTMFARIGGEEFAVLLQNVGTEQAALIADFLREEIERAKF
PYNPASGNVTVSIGVAATNGNFATTESLFEAADQALYQAKQNGRNQIAIHQGENK

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=348, Percent_Identity=28.1609195402299, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787802, Length=138, Percent_Identity=34.0579710144928, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=36.4705882352941, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=35, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=30.8176100628931, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=223, Percent_Identity=26.457399103139, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=195, Percent_Identity=30.2564102564103, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=29.4797687861272, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=30.4878048780488, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=33.9506172839506, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=29.9465240641711, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=34.5679012345679, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=180, Percent_Identity=31.1111111111111, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081974, Length=185, Percent_Identity=26.4864864864865, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43069; Mature: 43069

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCEEEEECCCCC
>Mature Secondary Structure
MFELVNSYIDSLALFLAILFIQGMSFRKIRQYRPNWFQNGGRRLFLSILLGIYYGLAGIY
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHH
FIYEGAYENNPIIYTNMRILILMVTTVFGGRIPLVVTYLIMLFGRISFDVASPVTSRYVI
HHEECCCCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LMTLIFAACLLVSYWKKQRFSKFIALIILNFPAILYYFVNNFEQERILRGFEIIEYFLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FVTALLVFYACNYIDKSNLVIQNLTETAMTDSLTNLPNMRFFTQQFGWIFNKAQKRKKAL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SLFIIDIDHFKEINDFHGHQAGNTVLAQFSTILKNRTFPPRTMFARIGGEEFAVLLQNVG
HEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCC
TEQAALIADFLREEIERAKFPYNPASGNVTVSIGVAATNGNFATTESLFEAADQALYQAK
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
QNGRNQIAIHQGENK
HCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]