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The map label for this gene is ycdT [H]

Identifier: 116873346

GI number: 116873346

Start: 1959668

End: 1960804

Strand: Direct

Name: ycdT [H]

Synonym: lwe1930

Alternate gene names: 116873346

Gene position: 1959668-1960804 (Clockwise)

Preceding gene: 116873344

Following gene: 116873347

Centisome position: 69.64

GC content: 33.25

Gene sequence:

>1137_bases
ATGCCAAATATTCTTGATTCATTATGGAACAGCATGGCGCTTTTTTTATCAGCGCTTTTTTTTCACGGAATGGCGCTTCG
GTCCATTAGAGAAAAAAAGCCAGCTTGGTTCCAAATTAAATTTGCAAATGAAATTATTAATTATTCACTGGGCATTTATT
ATGGCTTTCTTGGTGTTTATTTTATTCTCCGCGGATTACCTGGCACAGACTCTGGAATTTATACAGATATGTTATTAAAT
ATTTTAATTGTTCTACATTTATTTTCATCAGCAGGACCAGCTACTATTGCGCTACTTCTTATTATTACGGGTAAACTTTT
TCTAGGTGATGCTCTTTTCGCTAATTTAGTTTATGTGTTTTTAATTATTGGATTTCATTTTATTTGTATGGAAGTTGCTA
AATTACGACTTAGTTCTGTTCAAAAAGTCATTTTAGTCAAGCTTAGTGCAGTACCACTTATGCTTTTTTACTTACATCAA
AAAATACACCTCGAATATACACTAGATGACCTGCCTACTTGGATTCTTTACTTTTTGGTCTCTTTTTTTATTACTTTTAT
TCTTATTTCAGCAGCTTATTATATTGATACATCGAATAAGCTAATATATGATTTACAACAATCAACAATCCTTGATCCAC
TTACAGGCTTAACGAATTTCCGTCATTTTGACAAAACATTTGAAACCGCCTTTCATCATGCCACTTTAAAAAAATCGAAT
TTAAGTATCATCATTATTGATATTGATTATTTTAAACGGGTAAATGATACATATGGCCATTTAGTAGGAAATAGTGTTTT
ATCTACTTTTAGTCAAATGCTTTTAAAAATTAGCTTTCCACCAAATACAGTTATCTCTCGCATTGGAGGCGAAGAATTTG
CGATTATCTTGCCAAATATTAATGTGAGCGAAACAGAGCATTTGGCAGAGAAAATCCGACGAAAAGTCGAAAAAATCGAG
ATTCCAATTGCTGCATCTGGTTCAGTTATTACTATTTCTGCTGGAATCGCGAATTATGATGGGCACAATTATCCAACAGC
GGATGAATTACTCCATGCAGCTGATCAAGCCCTTTATAAAGCAAAACGGAATGGGCGAAATCAAGTACACATTCGTCAAA
CAGAGCCAGTTATTTAA

Upstream 100 bases:

>100_bases
GTTTGATGAACTACGTAATGCTAAAATTCTTATATTTTTTGAAATCTGTCTAAATTAACTTTTCAGCTGATTAGTTATGC
TGAGATATGGGAGATTTTTT

Downstream 100 bases:

>100_bases
GAGAGTAAAAAGGAGATGAGTCCGTTTGTTTGAACTAGTTAATTCATACATAGACAGTTTAGCACTCTTTTTAGCTATCC
TTTTTATTCAAGGGATGTCT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFANEIINYSLGIYYGFLGVYFILRGLPGTDSGIYTDMLLN
ILIVLHLFSSAGPATIALLLIITGKLFLGDALFANLVYVFLIIGFHFICMEVAKLRLSSVQKVILVKLSAVPLMLFYLHQ
KIHLEYTLDDLPTWILYFLVSFFITFILISAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFDKTFETAFHHATLKKSN
LSIIIIDIDYFKRVNDTYGHLVGNSVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKIE
IPIAASGSVITISAGIANYDGHNYPTADELLHAADQALYKAKRNGRNQVHIRQTEPVI

Sequences:

>Translated_378_residues
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFANEIINYSLGIYYGFLGVYFILRGLPGTDSGIYTDMLLN
ILIVLHLFSSAGPATIALLLIITGKLFLGDALFANLVYVFLIIGFHFICMEVAKLRLSSVQKVILVKLSAVPLMLFYLHQ
KIHLEYTLDDLPTWILYFLVSFFITFILISAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFDKTFETAFHHATLKKSN
LSIIIIDIDYFKRVNDTYGHLVGNSVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKIE
IPIAASGSVITISAGIANYDGHNYPTADELLHAADQALYKAKRNGRNQVHIRQTEPVI
>Mature_377_residues
PNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFANEIINYSLGIYYGFLGVYFILRGLPGTDSGIYTDMLLNI
LIVLHLFSSAGPATIALLLIITGKLFLGDALFANLVYVFLIIGFHFICMEVAKLRLSSVQKVILVKLSAVPLMLFYLHQK
IHLEYTLDDLPTWILYFLVSFFITFILISAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFDKTFETAFHHATLKKSNL
SIIIIDIDYFKRVNDTYGHLVGNSVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNINVSETEHLAEKIRRKVEKIEI
PIAASGSVITISAGIANYDGHNYPTADELLHAADQALYKAKRNGRNQVHIRQTEPVI

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=39.2638036809816, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=39.8773006134969, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=203, Percent_Identity=33.9901477832512, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787802, Length=154, Percent_Identity=35.0649350649351, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=34.2391304347826, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=213, Percent_Identity=30.0469483568075, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=29.2817679558011, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787816, Length=179, Percent_Identity=35.195530726257, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=29.608938547486, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=32.7044025157233, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=171, Percent_Identity=29.8245614035088, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=30.2469135802469, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=30.8176100628931, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42718; Mature: 42586

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFANEIINYSLGIYYGFLGVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
FILRGLPGTDSGIYTDMLLNILIVLHLFSSAGPATIALLLIITGKLFLGDALFANLVYVF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIIGFHFICMEVAKLRLSSVQKVILVKLSAVPLMLFYLHQKIHLEYTLDDLPTWILYFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
SFFITFILISAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFDKTFETAFHHATLKKSN
HHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
LSIIIIDIDYFKRVNDTYGHLVGNSVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNI
CEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEECCC
NVSETEHLAEKIRRKVEKIEIPIAASGSVITISAGIANYDGHNYPTADELLHAADQALYK
CCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AKRNGRNQVHIRQTEPVI
HHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
PNILDSLWNSMALFLSALFFHGMALRSIREKKPAWFQIKFANEIINYSLGIYYGFLGVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
FILRGLPGTDSGIYTDMLLNILIVLHLFSSAGPATIALLLIITGKLFLGDALFANLVYVF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIIGFHFICMEVAKLRLSSVQKVILVKLSAVPLMLFYLHQKIHLEYTLDDLPTWILYFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
SFFITFILISAAYYIDTSNKLIYDLQQSTILDPLTGLTNFRHFDKTFETAFHHATLKKSN
HHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
LSIIIIDIDYFKRVNDTYGHLVGNSVLSTFSQMLLKISFPPNTVISRIGGEEFAIILPNI
CEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEECCC
NVSETEHLAEKIRRKVEKIEIPIAASGSVITISAGIANYDGHNYPTADELLHAADQALYK
CCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
AKRNGRNQVHIRQTEPVI
HHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]