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The map label for this gene is ponA [H]

Identifier: 116873327

GI number: 116873327

Start: 1940065

End: 1942578

Strand: Direct

Name: ponA [H]

Synonym: lwe1911

Alternate gene names: 116873327

Gene position: 1940065-1942578 (Clockwise)

Preceding gene: 116873326

Following gene: 116873344

Centisome position: 68.94

GC content: 39.18

Gene sequence:

>2514_bases
ATGGCAGATAAACCGCAGACAAGATCTCAGTATCGCAGCAAAAAAAATGGAAATTCTAAAAAGAATCCATCTAAACGAGG
AAAAAGAGTTGCAGCTAATATTTTTAAAACTATTTTTTTCTTAGGACTATTTTTAGCCTTTTTTGGTGTTGCGGCTGGTG
CAACAGTCTTTTATAGTTATGCAAAAGATGCACCAAAACTGACCGACTCCAAGCTACGAGATCCACTTTCATCTAAATTA
CTTGATAAAGATGGCAAGGTTTTCGCAGAAGTTGGAACCGAACGGCGGGAATATATTGAATACAAAGATATACCTGAAGC
ACTAAAAAATGCGATTCTAACAACAGAAGATGCGCGTTTTTATGAACATGATGGGATTGACCCTATTCGACTTGGCGGAG
CAGTCATTGCTAATGTCAAAGACGGTTTTGGTTCAGAAGGTGCAAGTACACTTTCTCAGCAAATCATCAAGATGTCTTAT
CTTGATTACACCAACAAAACATTAGCGCGAAAAGCACAAGAAGCATGGCTTGCTCTTGAATTAGAGCAGAAATATAGCAA
AAATGATATTCTAGAAATTTATGTCAATAAAGTCTATATGTCAGACCGTGTTCACGGGATGCAAACCGCTTCCGAACATT
ATTTTGGCAAAAGCGTTAAAGACATTAGTTTAGCACAAACAGCGCTCCTTGCTGGCATGCCTCAAAGTCCGAATAATTAC
AATCCGTATGAACATCCAGAAGCAGCCAAAAAACGTCGTGATCAAGTGTTAACTAATATGTATTCTCATAATAAAATTAC
AAAAGATGAGATGACGGCAGCACAAAAAACACCTATCAACTCCGGACTACGCTCTCAAAAAGATCGTGAAGATAAAATTT
ACAAATACGATTCTTATGTAACGCAAGTTTTAAGTGAGATTCCGAAAGAATATGATGTCTACCGTGATGGTCTAACTATC
CACACTGCACTTGATCGTAGCGCACAAGAATACACCGAAAAAATGCTTAATACAAATGAAATCGTTAATTTCTCTGATAA
AGAAATGCAAGCCGGTATCGTGCTTCAAGATACAAAAAACGGTCGAGTTCAAGCAATTGGTGGCGGACGTAATCAAAAGG
TAACACGTGGTTATAATTACGCAACGCAAGTGAAGCGTTCTGTTGGTTCCACGATGAAACCTATTGCCGATTATGGTCCA
GCTTTTGAATATCTAGACTGGTCTACTGCACATATTTTAGAAGATGAACCTTATACGTATACTGGTGGAACACCTATTAA
TAACTGGGATTTCGGATATAAAGGTCCTATCTCTGTCAGACAAGCGCTTTATCAATCACGTAATATTCCAGCACTTAAAA
CTCTACAAGCTGTTGGACTCGAAAAATCCGAGCAATTTGTTAATAAACTTGGTATCACTTATGATAAAGGTCAAAACGTA
GAATCTAATGCTATCGGAGCAAATAGTTCCAATCCAATGCAAATGGCTGGTGCTTATGCTGCATTTGGTAATAAAGGTAT
TTACAATAAACCTCATACTATTATCAAAATTGTCCTATCTGATGGCGAAACTGAAATTGATACAGAACCAAAAAGCACTG
TAGCTATGAAAGAATCAACTGCTTATATGGTCTCTGATGTTCTAAAAGATGTTCTATCTATCGGAACAGGTACTTCAGCA
GCCGTTCCAGGAGTGCCTGCTGCAGGTAAAACTGGTACTACAAATATCCCACCTGAATTCACTTCGAAGTACTACTACCC
TGGTGGTGCCGCTCGTGACTCATGGTTTGCTGGATATACCACAAATTATTCTATCGCTGTATGGACTGGTTATGATGATA
AGAAAAAATACGTTTCAGCAAGTGAACAAAAAATTGCTCAACGTCTGTTTAGTAAATTAATGGCTCATGCATCTGCTAAT
AAAAATACGGCAGACTTCAAAATGCCAAGTAATGTCGTTTCTGTTCCAATTTTAAAAGGTAGTAACCCAATTGCTCGTGC
TGCATCTGGTACTTCTAGTGATAAGGTAAGCTACGAACTATTCTTAGCTGGAACTGCTCCAACGAAAACTGCTTCCACTC
CAGAAGATGAGAAGAAGAAAAAAGAAGCAGAAGCGAAGAAAAAAGCGGAAGAAGAAGCGAAGAAGAAAAAAGAAGATGAG
AAAAAATCAGAAGCAGATGATGAAGCAGAAGCAAAGAAAAAAGCAGAAGAAGAGGCTAAGAAGAAGGCTGAGGAAGAGGA
AGCCGCAAAAGCCCTCACTGCCCCTGCTGGCTTAAGAGCAAGTTATGACGCAAACTCTAAGCAAATTAATGTTTCTTGGG
CTGCAGTAGATGGTGCGACTTATGAAGTGAATGTCAATGGAACCACAACGACAGTATCTTCTACATCCGTTTCAGTAAGT
GGTGGAAACCCTGGTGATACAATCTCGATCAATGTTGTCGCCGTAAAAGATGGTAAGAAGAGTCCAGCATCCTCCACCAC
CGTCAAGATTCCAGCTGAAGAGCCCTCTGATTAA

Upstream 100 bases:

>100_bases
CAGACCAAATTCCTTATGGTCTAAATCCAAGGCTTGACTTCTTGAAATCAATCGAGAAATTATACTTCTAGTCAGCTTTT
TTAAAGGAGAGAGATTTTTA

Downstream 100 bases:

>100_bases
TAAAGTAATAACAAAAAGAGAAGACGAGTTTCGTCTTCTCTTTTTGTTATATCCCTATAAGATTATTTTTTTGCAATTAA
TTTACGTTGCTCAAGTGTCA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 837; Mature: 836

Protein sequence:

>837_residues
MADKPQTRSQYRSKKNGNSKKNPSKRGKRVAANIFKTIFFLGLFLAFFGVAAGATVFYSYAKDAPKLTDSKLRDPLSSKL
LDKDGKVFAEVGTERREYIEYKDIPEALKNAILTTEDARFYEHDGIDPIRLGGAVIANVKDGFGSEGASTLSQQIIKMSY
LDYTNKTLARKAQEAWLALELEQKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKSVKDISLAQTALLAGMPQSPNNY
NPYEHPEAAKKRRDQVLTNMYSHNKITKDEMTAAQKTPINSGLRSQKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTI
HTALDRSAQEYTEKMLNTNEIVNFSDKEMQAGIVLQDTKNGRVQAIGGGRNQKVTRGYNYATQVKRSVGSTMKPIADYGP
AFEYLDWSTAHILEDEPYTYTGGTPINNWDFGYKGPISVRQALYQSRNIPALKTLQAVGLEKSEQFVNKLGITYDKGQNV
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTIIKIVLSDGETEIDTEPKSTVAMKESTAYMVSDVLKDVLSIGTGTSA
AVPGVPAAGKTGTTNIPPEFTSKYYYPGGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRLFSKLMAHASAN
KNTADFKMPSNVVSVPILKGSNPIARAASGTSSDKVSYELFLAGTAPTKTASTPEDEKKKKEAEAKKKAEEEAKKKKEDE
KKSEADDEAEAKKKAEEEAKKKAEEEEAAKALTAPAGLRASYDANSKQINVSWAAVDGATYEVNVNGTTTTVSSTSVSVS
GGNPGDTISINVVAVKDGKKSPASSTTVKIPAEEPSD

Sequences:

>Translated_837_residues
MADKPQTRSQYRSKKNGNSKKNPSKRGKRVAANIFKTIFFLGLFLAFFGVAAGATVFYSYAKDAPKLTDSKLRDPLSSKL
LDKDGKVFAEVGTERREYIEYKDIPEALKNAILTTEDARFYEHDGIDPIRLGGAVIANVKDGFGSEGASTLSQQIIKMSY
LDYTNKTLARKAQEAWLALELEQKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKSVKDISLAQTALLAGMPQSPNNY
NPYEHPEAAKKRRDQVLTNMYSHNKITKDEMTAAQKTPINSGLRSQKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTI
HTALDRSAQEYTEKMLNTNEIVNFSDKEMQAGIVLQDTKNGRVQAIGGGRNQKVTRGYNYATQVKRSVGSTMKPIADYGP
AFEYLDWSTAHILEDEPYTYTGGTPINNWDFGYKGPISVRQALYQSRNIPALKTLQAVGLEKSEQFVNKLGITYDKGQNV
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTIIKIVLSDGETEIDTEPKSTVAMKESTAYMVSDVLKDVLSIGTGTSA
AVPGVPAAGKTGTTNIPPEFTSKYYYPGGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRLFSKLMAHASAN
KNTADFKMPSNVVSVPILKGSNPIARAASGTSSDKVSYELFLAGTAPTKTASTPEDEKKKKEAEAKKKAEEEAKKKKEDE
KKSEADDEAEAKKKAEEEAKKKAEEEEAAKALTAPAGLRASYDANSKQINVSWAAVDGATYEVNVNGTTTTVSSTSVSVS
GGNPGDTISINVVAVKDGKKSPASSTTVKIPAEEPSD
>Mature_836_residues
ADKPQTRSQYRSKKNGNSKKNPSKRGKRVAANIFKTIFFLGLFLAFFGVAAGATVFYSYAKDAPKLTDSKLRDPLSSKLL
DKDGKVFAEVGTERREYIEYKDIPEALKNAILTTEDARFYEHDGIDPIRLGGAVIANVKDGFGSEGASTLSQQIIKMSYL
DYTNKTLARKAQEAWLALELEQKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKSVKDISLAQTALLAGMPQSPNNYN
PYEHPEAAKKRRDQVLTNMYSHNKITKDEMTAAQKTPINSGLRSQKDREDKIYKYDSYVTQVLSEIPKEYDVYRDGLTIH
TALDRSAQEYTEKMLNTNEIVNFSDKEMQAGIVLQDTKNGRVQAIGGGRNQKVTRGYNYATQVKRSVGSTMKPIADYGPA
FEYLDWSTAHILEDEPYTYTGGTPINNWDFGYKGPISVRQALYQSRNIPALKTLQAVGLEKSEQFVNKLGITYDKGQNVE
SNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTIIKIVLSDGETEIDTEPKSTVAMKESTAYMVSDVLKDVLSIGTGTSAA
VPGVPAAGKTGTTNIPPEFTSKYYYPGGAARDSWFAGYTTNYSIAVWTGYDDKKKYVSASEQKIAQRLFSKLMAHASANK
NTADFKMPSNVVSVPILKGSNPIARAASGTSSDKVSYELFLAGTAPTKTASTPEDEKKKKEAEAKKKAEEEAKKKKEDEK
KSEADDEAEAKKKAEEEAKKKAEEEEAAKALTAPAGLRASYDANSKQINVSWAAVDGATYEVNVNGTTTTVSSTSVSVSG
GNPGDTISINVVAVKDGKKSPASSTTVKIPAEEPSD

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=561, Percent_Identity=30.1247771836007, Blast_Score=211, Evalue=2e-55,
Organism=Escherichia coli, GI87082258, Length=322, Percent_Identity=31.055900621118, Blast_Score=172, Evalue=1e-43,
Organism=Escherichia coli, GI1788867, Length=583, Percent_Identity=26.4150943396226, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=29.6969696969697, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 91716; Mature: 91585

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPQTRSQYRSKKNGNSKKNPSKRGKRVAANIFKTIFFLGLFLAFFGVAAGATVFYSY
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDAPKLTDSKLRDPLSSKLLDKDGKVFAEVGTERREYIEYKDIPEALKNAILTTEDARF
HCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCH
YEHDGIDPIRLGGAVIANVKDGFGSEGASTLSQQIIKMSYLDYTNKTLARKAQEAWLALE
HHCCCCCEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEQKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKSVKDISLAQTALLAGMPQSPNNY
EHHHCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
NPYEHPEAAKKRRDQVLTNMYSHNKITKDEMTAAQKTPINSGLRSQKDREDKIYKYDSYV
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHH
TQVLSEIPKEYDVYRDGLTIHTALDRSAQEYTEKMLNTNEIVNFSDKEMQAGIVLQDTKN
HHHHHHCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCEECCCCCHHHCCEEEEECCC
GRVQAIGGGRNQKVTRGYNYATQVKRSVGSTMKPIADYGPAFEYLDWSTAHILEDEPYTY
CEEEEECCCCCCCEECCCCHHHHHHHHHCCHHHHHHHCCCCHHHCCCCHHEEECCCCEEE
TGGTPINNWDFGYKGPISVRQALYQSRNIPALKTLQAVGLEKSEQFVNKLGITYDKGQNV
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCEECCCCCC
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTIIKIVLSDGETEIDTEPKSTVAMKEST
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEHHHH
AYMVSDVLKDVLSIGTGTSAAVPGVPAAGKTGTTNIPPEFTSKYYYPGGAARDSWFAGYT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCEECEE
TNYSIAVWTGYDDKKKYVSASEQKIAQRLFSKLMAHASANKNTADFKMPSNVVSVPILKG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC
SNPIARAASGTSSDKVSYELFLAGTAPTKTASTPEDEKKKKEAEAKKKAEEEAKKKKEDE
CCCHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KKSEADDEAEAKKKAEEEAKKKAEEEEAAKALTAPAGLRASYDANSKQINVSWAAVDGAT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEECCEE
YEVNVNGTTTTVSSTSVSVSGGNPGDTISINVVAVKDGKKSPASSTTVKIPAEEPSD
EEEECCCCEEEEECCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
ADKPQTRSQYRSKKNGNSKKNPSKRGKRVAANIFKTIFFLGLFLAFFGVAAGATVFYSY
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDAPKLTDSKLRDPLSSKLLDKDGKVFAEVGTERREYIEYKDIPEALKNAILTTEDARF
HCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCH
YEHDGIDPIRLGGAVIANVKDGFGSEGASTLSQQIIKMSYLDYTNKTLARKAQEAWLALE
HHCCCCCEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEQKYSKNDILEIYVNKVYMSDRVHGMQTASEHYFGKSVKDISLAQTALLAGMPQSPNNY
EHHHCCCCCEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
NPYEHPEAAKKRRDQVLTNMYSHNKITKDEMTAAQKTPINSGLRSQKDREDKIYKYDSYV
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHH
TQVLSEIPKEYDVYRDGLTIHTALDRSAQEYTEKMLNTNEIVNFSDKEMQAGIVLQDTKN
HHHHHHCCHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCEECCCCCHHHCCEEEEECCC
GRVQAIGGGRNQKVTRGYNYATQVKRSVGSTMKPIADYGPAFEYLDWSTAHILEDEPYTY
CEEEEECCCCCCCEECCCCHHHHHHHHHCCHHHHHHHCCCCHHHCCCCHHEEECCCCEEE
TGGTPINNWDFGYKGPISVRQALYQSRNIPALKTLQAVGLEKSEQFVNKLGITYDKGQNV
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCEECCCCCC
ESNAIGANSSNPMQMAGAYAAFGNKGIYNKPHTIIKIVLSDGETEIDTEPKSTVAMKEST
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEHHHH
AYMVSDVLKDVLSIGTGTSAAVPGVPAAGKTGTTNIPPEFTSKYYYPGGAARDSWFAGYT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCEECEE
TNYSIAVWTGYDDKKKYVSASEQKIAQRLFSKLMAHASANKNTADFKMPSNVVSVPILKG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC
SNPIARAASGTSSDKVSYELFLAGTAPTKTASTPEDEKKKKEAEAKKKAEEEAKKKKEDE
CCCHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KKSEADDEAEAKKKAEEEAKKKAEEEEAAKALTAPAGLRASYDANSKQINVSWAAVDGAT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEECCEE
YEVNVNGTTTTVSSTSVSVSGGNPGDTISINVVAVKDGKKSPASSTTVKIPAEEPSD
EEEECCCCEEEEECCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]