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The map label for this gene is bfmBB [H]

Identifier: 116872805

GI number: 116872805

Start: 1377017

End: 1378264

Strand: Direct

Name: bfmBB [H]

Synonym: lwe1389

Alternate gene names: 116872805

Gene position: 1377017-1378264 (Clockwise)

Preceding gene: 116872804

Following gene: 116872807

Centisome position: 48.93

GC content: 38.46

Gene sequence:

>1248_bases
GTGGCAGTTGAAAAAATCACCATGCCCAAATTAGGGGAAAGTGTAACAGAAGGAACCATTAGTTCGTGGTTAGTTAAACC
AGGCGATACAGTAGAAAAATATGATGCAATTGCAGAAGTGCTAACCGACAAAGTAACAGCTGAAATTCCGTCTTCTTTTA
GCGGAACAATTAAAGAAATTTTAGCAGAAGAAGATGAAACATTAGAAGTAGGCGAAGTTATCTGCACCATCGAAACAGCA
GAGTCTGGCGGTTCAGAACCAGCCGATGAAGAAAAACAACCAGAAACAAAAAACGATGAAAAAAAAGAAACTAAACAAGT
GAAATTAGCAGAAGCACCGGCTAGTGGAAGATTTTCCCCAGCTGTACTACGAATTGCTGGCGAAAACAATATAGACTTAT
CCACTGTAGAAGGCACTGGTAAAGGTGGCCGAATTACTAGAAAAGATTTACTTCAAGTAATAGAAAATGGGCCAGTAACT
ACTAAATCAGAACCAAAGAATGTGACACAAGAAAAAACTGCAACTCCTGCGCCTATTCGTTCTGCAGCTGGTGACAAAGA
AATTCCAATAAATGGCGTAAGAAAAGCAATTGCGAAACATATGAGCGTCAGCAAACAAGAAATTCCGCACGCATGGATGA
TGGTGGAAGTTGATGCTACTGGTCTAGTGCGCTATCGTAATGCAGTAAAAGATACTTTCAAAAAAGAGGAAGGTTATTCC
TTAACTTATTTCGCGTTTTTCATAAAAGCAGTTGCGCAAGCCTTAAAAGAATTTCCGCAATTAAATAGTACTTGGGCAGG
CGATAAAATCATTGAGCATGCAAATATCAATATTTCAATTGCTATTGCAGCTGGCGATTTATTATATGTTCCGGTTATTA
AAAATGCAGATGAGAAATCTATTAAAGGGATTGCTCGTGAAATAAGTGAACTTGCTGAGAAAGCTCGGAGTGGCAAACTT
AGCCAAGCAGACATGGAAGGTGGTACATTCACTGTGAATAGTACTGGTTCCTTTGGCTCTGTGCAATCAATGGGAATTAT
TAATCACCCACAAGCAGCTATTCTTCAAGTAGAATCAATTGTAAAACGGCCAGTAATTGTGGATGATATGATCGCTGTTC
GTGATATGGTAAATCTTTGTTTATCCATCGATCACCGTATTTTAGACGGTTTACTCGCTGGGAAATTTTTACAAGCAATT
AAATCTGCTGTCGAAAAAATCTCTAAAGATAACACAGCGTTATATTAA

Upstream 100 bases:

>100_bases
GATGGAAAAACATTTCATGATTAATCCTGATAAAGTGGCGGATGCAATGAAAGAATTAGCGGAATTTTAGACCCGTTTTA
AATTAAAGGAGTGAAGACCC

Downstream 100 bases:

>100_bases
AGATGTTTTAATCTAGTTTTAGCGGGTATTGTTTAGTACATCTAGATCCATACCCCTAAACTCCCTAGATGTCTTGGGTA
GTACTTTCGGGTACTGCTCT

Product: branched-chain alpha-keto acid dehydrogenase E2 subunit (lipoamide acyltransferase)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETA
ESGGSEPADEEKQPETKNDEKKETKQVKLAEAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVT
TKSEPKNVTQEKTATPAPIRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDTFKKEEGYS
LTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAEKARSGKL
SQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIVDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAI
KSAVEKISKDNTALY

Sequences:

>Translated_415_residues
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETA
ESGGSEPADEEKQPETKNDEKKETKQVKLAEAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVT
TKSEPKNVTQEKTATPAPIRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDTFKKEEGYS
LTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAEKARSGKL
SQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIVDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAI
KSAVEKISKDNTALY
>Mature_414_residues
AVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEILAEEDETLEVGEVICTIETAE
SGGSEPADEEKQPETKNDEKKETKQVKLAEAPASGRFSPAVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVTT
KSEPKNVTQEKTATPAPIRSAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDTFKKEEGYSL
TYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKSIKGIAREISELAEKARSGKLS
QADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESIVKRPVIVDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAIK
SAVEKISKDNTALY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=33.7320574162679, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=30.2013422818792, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=30.2013422818792, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=30.9468822170901, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI19923748, Length=218, Percent_Identity=37.1559633027523, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=33.974358974359, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=32.3529411764706, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=26.8235294117647, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=32.9383886255924, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.2785388127854, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=398, Percent_Identity=31.1557788944724, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17538894, Length=295, Percent_Identity=29.4915254237288, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=30.4878048780488, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6324258, Length=443, Percent_Identity=26.1851015801354, Blast_Score=135, Evalue=8e-33,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=31.7865429234339, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=35.2941176470588, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=32.8828828828829, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=32.8828828828829, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 44853; Mature: 44721

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEI
CCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAEEDETLEVGEVICTIETAESGGSEPADEEKQPETKNDEKKETKQVKLAEAPASGRFSP
HCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCCC
AVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVTTKSEPKNVTQEKTATPAPIR
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCHH
SAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDTFKKEEGYS
HCCCCCCCCHHHHHHHHHHHHHCHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCC
LTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCEEEEEEEECCEEEEEEECCCCHHH
IKGIAREISELAEKARSGKLSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHHH
VKRPVIVDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAIKSAVEKISKDNTALY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVEKITMPKLGESVTEGTISSWLVKPGDTVEKYDAIAEVLTDKVTAEIPSSFSGTIKEI
CCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LAEEDETLEVGEVICTIETAESGGSEPADEEKQPETKNDEKKETKQVKLAEAPASGRFSP
HCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCCC
AVLRIAGENNIDLSTVEGTGKGGRITRKDLLQVIENGPVTTKSEPKNVTQEKTATPAPIR
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCHH
SAAGDKEIPINGVRKAIAKHMSVSKQEIPHAWMMVEVDATGLVRYRNAVKDTFKKEEGYS
HCCCCCCCCHHHHHHHHHHHHHCHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCC
LTYFAFFIKAVAQALKEFPQLNSTWAGDKIIEHANINISIAIAAGDLLYVPVIKNADEKS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCEEEEEEEECCEEEEEEECCCCHHH
IKGIAREISELAEKARSGKLSQADMEGGTFTVNSTGSFGSVQSMGIINHPQAAILQVESI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHHH
VKRPVIVDDMIAVRDMVNLCLSIDHRILDGLLAGKFLQAIKSAVEKISKDNTALY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]