Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 116872791

GI number: 116872791

Start: 1362339

End: 1363193

Strand: Direct

Name: folD [H]

Synonym: lwe1375

Alternate gene names: 116872791

Gene position: 1362339-1363193 (Clockwise)

Preceding gene: 116872790

Following gene: 116872792

Centisome position: 48.41

GC content: 36.26

Gene sequence:

>855_bases
ATGGGACAAATTATTGACGGTGAAAAATTAGCAAAAGAAATTCAAGAGCGAGTAACAACAGAAGTAGCAGAATTAGCAAA
AATCGATAAAAAACCAGGACTTGCAGTTGTGCTAGTAGGAGACAACCAAGCATCTCGTACATATGTAAGAAATAAACAAA
AGCGCACGGAAGAAGCAGGAATGAAATCCGTTTTGATTGAACTTCCTGAAACCGTTTCAGAAGAAAAATTACTAGAAGTA
GTAGAAGAATTAAATACGGATGATACAATTCATGGTATTCTTGTACAATTACCATTACCTAAACATATTTCAGAAGAAAA
AGTAATTGATACAATTAGTTATGACAAAGACGTTGACGGTTTCCACCCTGTGAATGTAGGTAATCTTTTCATAGGCAAAG
ATTCATTTGTTCCATGTACACCAGCAGGAATTATTGAATTAATTAAATCAACCGGCACTCAAATAGAAGGAAAACGCGCA
GTGGTAATTGGTAGAAGTAACATAGTGGGAAAACCCGTAGCACAATTATTATTGAATGAAAATGCAACTGTTACTATAGC
GCATAGTCGTACAAAAAACTTACCAGAAGTAGCAAAAGAAGCGGATATTCTTGTCGTTGCAACAGGATTAGCTAAATTTG
TGAAAAAAGAATATATTAAACCCGGTGCTATTGTTATTGACGTTGGTATGGACCGCGATGAAAACAATAAATTATGTGGT
GATGTAGATTTTGATGATGTTATAGAAGAAGCCGGATTCATCACGCCCGTTCCTGGTGGAGTTGGCCCGATGACAATCAC
AATGCTACTTGCCAACACATTAAAAGCAGCAAAACGCATTTGGAAGATGAATTGA

Upstream 100 bases:

>100_bases
TAAATAAACTTGATTGAGTGCGAGGATATTCCTTGCACTCCCTTATTTCGCGATTTTTTAAGATAAAACTTGGAAATGAA
ATTTTAAGGGGGCAATAAGT

Downstream 100 bases:

>100_bases
TTAATTGGATGTGAAATGATGGAACAAGATAAATATTTAACGGTAGCAGCGATAACCAAATATATTGAAAAAAAATTCGA
CGTAGATCCGTATATGAAAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MGQIIDGEKLAKEIQERVTTEVAELAKIDKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPETVSEEKLLEV
VEELNTDDTIHGILVQLPLPKHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRA
VVIGRSNIVGKPVAQLLLNENATVTIAHSRTKNLPEVAKEADILVVATGLAKFVKKEYIKPGAIVIDVGMDRDENNKLCG
DVDFDDVIEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN

Sequences:

>Translated_284_residues
MGQIIDGEKLAKEIQERVTTEVAELAKIDKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPETVSEEKLLEV
VEELNTDDTIHGILVQLPLPKHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRA
VVIGRSNIVGKPVAQLLLNENATVTIAHSRTKNLPEVAKEADILVVATGLAKFVKKEYIKPGAIVIDVGMDRDENNKLCG
DVDFDDVIEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
>Mature_283_residues
GQIIDGEKLAKEIQERVTTEVAELAKIDKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPETVSEEKLLEVV
EELNTDDTIHGILVQLPLPKHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAV
VIGRSNIVGKPVAQLLLNENATVTIAHSRTKNLPEVAKEADILVVATGLAKFVKKEYIKPGAIVIDVGMDRDENNKLCGD
VDFDDVIEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=46.4163822525597, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=47.098976109215, Blast_Score=248, Evalue=3e-66,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.945945945946, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI36796743, Length=225, Percent_Identity=28, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=50.9025270758123, Blast_Score=264, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=43.6860068259386, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=45.6747404844291, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=45.2702702702703, Blast_Score=249, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6322933, Length=328, Percent_Identity=28.3536585365854, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.7128027681661, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.7128027681661, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.7128027681661, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.7128027681661, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=43.7710437710438, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=43.7710437710438, Blast_Score=232, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30912; Mature: 30781

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQIIDGEKLAKEIQERVTTEVAELAKIDKKPGLAVVLVGDNQASRTYVRNKQKRTEEAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
MKSVLIELPETVSEEKLLEVVEELNTDDTIHGILVQLPLPKHISEEKVIDTISYDKDVDG
HHHHHHHCCHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAVVIGRSNIVGKPVAQLLLNE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEECCCCCHHHHHHHHHCC
NATVTIAHSRTKNLPEVAKEADILVVATGLAKFVKKEYIKPGAIVIDVGMDRDENNKLCG
CCEEEEECCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DVDFDDVIEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
CCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GQIIDGEKLAKEIQERVTTEVAELAKIDKKPGLAVVLVGDNQASRTYVRNKQKRTEEAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
MKSVLIELPETVSEEKLLEVVEELNTDDTIHGILVQLPLPKHISEEKVIDTISYDKDVDG
HHHHHHHCCHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAVVIGRSNIVGKPVAQLLLNE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEECCCCCHHHHHHHHHCC
NATVTIAHSRTKNLPEVAKEADILVVATGLAKFVKKEYIKPGAIVIDVGMDRDENNKLCG
CCEEEEECCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DVDFDDVIEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
CCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]