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The map label for this gene is rbfA [H]

Identifier: 116872758

GI number: 116872758

Start: 1335170

End: 1335514

Strand: Direct

Name: rbfA [H]

Synonym: lwe1342

Alternate gene names: 116872758

Gene position: 1335170-1335514 (Clockwise)

Preceding gene: 116872757

Following gene: 116872759

Centisome position: 47.45

GC content: 38.84

Gene sequence:

>345_bases
TTGAACGTACGAGCAAATCGTGTCAGTGAGCAAATGAAAAAAGAATTGGGCGATATTTTAAATCGTAAAATTAAAGATCC
ACGTTTAGGTTTTGTGACAGTAACTGGTGTAGATGTAACTGGGGACTTACAAGAAGCTAAGGTGTTCATTTCCATTCTTG
GTACCGATAAGGAAAAAGAAAATACGTTACTCGCACTTGCGAAAGCGCATGGCTTTATCCGCTCAGAAATCGGTCGCCGT
ATTCGACTTCGTAAAGTTCCAGAAATGTCTTTTGAAATAGATAATTCCATCGCTTACGGAAATCGAATCGATGAATTGCT
TCGCGACTTAAATAGCGATCAATAA

Upstream 100 bases:

>100_bases
GGAAAAAGAAGCTAGAGAAGTACTTGCTTTTCTCGATTCTTTTCCTGAATGGGAGCGAGCTGAGACAGTAATGGAGAAGT
TATAAATGAGGTGAATACAA

Downstream 100 bases:

>100_bases
TAAATTAAAGTTTAAGGCAATCCCTTTATCTTCTCAGTCAAACGGGATATAAATGGAGAATTGTCTTAAACTTTTTTTCT
TAGAAGGAGGAGCTTGATGA

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNSDQ

Sequences:

>Translated_114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNSDQ
>Mature_114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNSDQ

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=108, Percent_Identity=46.2962962962963, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 12941; Mature: 12941

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHEECCCCCCC
NTLLALAKAHGFIRSEIGRRIRLRKVPEMSFEIDNSIAYGNRIDELLRDLNSDQ
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHEECCCCCCC
NTLLALAKAHGFIRSEIGRRIRLRKVPEMSFEIDNSIAYGNRIDELLRDLNSDQ
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]