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The map label for this gene is topA [H]

Identifier: 116872708

GI number: 116872708

Start: 1280998

End: 1283076

Strand: Direct

Name: topA [H]

Synonym: lwe1292

Alternate gene names: 116872708

Gene position: 1280998-1283076 (Clockwise)

Preceding gene: 116872707

Following gene: 116872709

Centisome position: 45.52

GC content: 36.17

Gene sequence:

>2079_bases
ATGGCAGATTATTTAGTGATTGTAGAGTCACCTGCAAAAGCAAAAACAATTGAAAAATATTTAGGTAAGAAATTTAAAGT
CAAAGCTTCGATGGGACATGTTAGAGATTTACCAAAAAGCCAAATGGGCGTAGATACAGAACATGACTATGAACCACGTT
ATATTACGATTCGCGGAAAAGGCCCTGTTTTAAAAGAATTAAAACAAGCAGCTAAAAAAGCGAAAAAAGTCTATCTCGCA
GCCGATCCAGATCGCGAAGGGGAAGCAATTGCGTGGCATCTAGCTAACAGTCTTGAGTTAGATCAATCAGACAAATTACG
TGTAGTATTTAATGAAATAACCAAAGAAGCAGTCAAAGAATCATTCAAAACTCCTCGAAAAATTGATATGGATCTTGTAA
ATGCTCAGCAAGCTCGCAGGATTTTGGATCGCTTGGTTGGGTATAACATTAGTCCTATACTTTGGAAAAAAGTGAAAAAA
GGTCTAAGTGCTGGACGAGTTCAATCAATAGCACTTCGCATCATTATTGATAGAGAAAAAGAAATAAATAATTTTAAACC
CGAAGAATATTGGACAATTGATGGTAACTTCCTTAAAGGAAAGAAAAAATTCCAAGCTAATTTTTATGGAGTGGAAGGTA
AAAAGAAAAAACTATCTACTGCGGATGATGTGAAAGAAGTAATGTCTGCAATTAAGGGAAAAACATTTGATGTAACAGAT
GTAACTAAAAAAGAACGACTTAGAAACCCAGCTGCTCCATTTACAACATCTAGTTTGCAACAAGAAGCAGCACGTAAATT
AAATTATCGTACTAGAAAAACAATGATGCTTGCTCAACAATTATACGAAGGAATCACCCTTGGAAAACAGGGTACAGTAG
GTTTAATCACTTATATGCGTACTGACTCCACACGTATTGCAGATTCTGCTATTTTAGAAGCAGGTAATTTTATTAAAGAA
ACTTATGGACCTGAATTTTCTCGTAACCATAAACGTTCAGATAAAAATGCTAAAGGTGCACAAGATGCCCATGAAGCAAT
CCGTCCAACCAGCGCTATGAGAAGCCCGCAAGAAGTAAAAGAATATCTAACGCGTGATCAACTTCGCTTATATCGTCTAA
TTTGGGAACGATTTATTGCTAGCCAAATGACGCCAGCTGTCCTTGATACGATGCGAGTAGACCTTGATAATAATGGCGTT
AACTTTAGAGCAAACGGATCAAAAATTAAATTCCATGGATTTATGAAAGTTTATGTGGAAAGTAACGATGACAATACGGA
AGAAAAAGAAAATATTTTACCGGATTTGAAAAAAGGAGACAAGGTACAGTCCGAATCACTAGAACAGCGTCAGCATTTTA
CGCAGCCACCTCCACGTTATACAGAAGCAAGGTTAGTAAAAACGCTTGAAGAAATTGGCATCGGTCGGCCATCTACTTAT
TCACCGACGCTAGATACGATTCAACGAAGAAATTATGTTTCTTTAACAAACAAACGATTTGTTCCTACAGAACTTGGTGA
AATAGTTAATGAAATGATTGAAGAATATTTCCCTGAAATTTTAGATGTGAAGTTCACTGCGAATATGGAAAGTGAATTAG
ATGAAGTAGAGCACGGTGAAGTGGAATGGGTAAAAGTTATTGATGAATTTTATAAACAATTTGAACCAAATGTCATAAAA
GCAGATGCAGAAATGGAAAAAATTGAAATTAAAGACGAACCAGCTGGTATCGATTGCGATCTTTGTGGAGCGCCAATGGT
ATACAAAATGGGAAAATATGGGAAATTTCTTGCATGTAGCAGGTTCCCAGATTGCCGAAATACGAAAGCTATCGTCAAAG
AAATCGGTGTGACTTGTCCAAAATGTGAGAAAGGACATGTTATAGAAAGAAAAAGTAAGAAAAAACGTATTTTCTATGGT
TGTGACCGTTATCCAGATTGCGATTACGTTTCTTGGGATAAACCTGTTGAACGAGCTTGTCCGAAATGTAATGAACGAGC
ACTAGTAGAGAAGAAATTGAAAAAAGGTGTACAAGTACAATGTACAAATTGTGATTACAAAGAATCAACCCAACAATAA

Upstream 100 bases:

>100_bases
CAGCATGCAAACTTATTGAACGCGAGGCTAGTACTTTGAGAAAATTACTACAACTGTTAAAGCCCGTGTATTGACCCAAA
TCAGGGAGGAATATATTGAT

Downstream 100 bases:

>100_bases
ATGGAAAATGGCAGCACTTGGATAGTTCCTGTGTTGCCATTGTTGCGCGAGGAGGCGGACAAATGGAAAAAAGTGTTAAT
GTAATTGGAGCAGGATTAGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLA
ADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKK
GLSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVEGKKKKLSTADDVKEVMSAIKGKTFDVTD
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEAGNFIKE
TYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGV
NFRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
SPTLDTIQRRNYVSLTNKRFVPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKQFEPNVIK
ADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYG
CDRYPDCDYVSWDKPVERACPKCNERALVEKKLKKGVQVQCTNCDYKESTQQ

Sequences:

>Translated_692_residues
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLA
ADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKK
GLSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVEGKKKKLSTADDVKEVMSAIKGKTFDVTD
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEAGNFIKE
TYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGV
NFRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
SPTLDTIQRRNYVSLTNKRFVPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKQFEPNVIK
ADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYG
CDRYPDCDYVSWDKPVERACPKCNERALVEKKLKKGVQVQCTNCDYKESTQQ
>Mature_691_residues
ADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGKGPVLKELKQAAKKAKKVYLAA
DPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKG
LSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVEGKKKKLSTADDVKEVMSAIKGKTFDVTDV
TKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEAGNFIKET
YGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVKEYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVN
FRANGSKIKFHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTYS
PTLDTIQRRNYVSLTNKRFVPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKQFEPNVIKA
DAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGC
DRYPDCDYVSWDKPVERACPKCNERALVEKKLKKGVQVQCTNCDYKESTQQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=627, Percent_Identity=25.9968102073365, Blast_Score=133, Evalue=7e-31,
Organism=Homo sapiens, GI4507635, Length=560, Percent_Identity=21.9642857142857, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=37.3670212765958, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1788061, Length=520, Percent_Identity=23.2692307692308, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI48994931, Length=107, Percent_Identity=39.2523364485981, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=549, Percent_Identity=26.4116575591985, Blast_Score=139, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=23.7154150197628, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=481, Percent_Identity=23.2848232848233, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=23.8021638330757, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640096, Length=513, Percent_Identity=23.0019493177388, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79375; Mature: 79244

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGK
CCCEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVLKELKQAAKKAKKVYLAADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
SFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKGLSAGRVQSIALRIIIDREK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHEEEEEEEEECHH
EINNFKPEEYWTIDGNFLKGKKKFQANFYGVEGKKKKLSTADDVKEVMSAIKGKTFDVTD
HCCCCCCCCEEEECCCEECCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMR
CHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRIADSAILEAGNFIKETYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHH
EYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVNFRANGSKIKFHGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEECCCEEEEEEEEEEEEE
SNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPTLDTIQRRNYVSLTNKRFVPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGE
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCC
VEWVKVIDEFYKQFEPNVIKADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACS
HHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
RFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGCDRYPDCDYVSWDKPVERAC
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHC
PKCNERALVEKKLKKGVQVQCTNCDYKESTQQ
CCCCHHHHHHHHHHCCCEEEECCCCCHHCCCC
>Mature Secondary Structure 
ADYLVIVESPAKAKTIEKYLGKKFKVKASMGHVRDLPKSQMGVDTEHDYEPRYITIRGK
CCEEEEEECCCHHHHHHHHCCCEEEEEEHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVLKELKQAAKKAKKVYLAADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKE
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
SFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVKKGLSAGRVQSIALRIIIDREK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHEEEEEEEEECHH
EINNFKPEEYWTIDGNFLKGKKKFQANFYGVEGKKKKLSTADDVKEVMSAIKGKTFDVTD
HCCCCCCCCEEEECCCEECCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
VTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMR
CHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
TDSTRIADSAILEAGNFIKETYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEVK
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCHHHHH
EYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVNFRANGSKIKFHGFMKVYVE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEECCCEEEEEEEEEEEEE
SNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTY
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPTLDTIQRRNYVSLTNKRFVPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGE
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHCCCC
VEWVKVIDEFYKQFEPNVIKADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACS
HHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
RFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGCDRYPDCDYVSWDKPVERAC
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHC
PKCNERALVEKKLKKGVQVQCTNCDYKESTQQ
CCCCHHHHHHHHHHCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]