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The map label for this gene is cbiF [H]

Identifier: 116872570

GI number: 116872570

Start: 1163421

End: 1164170

Strand: Direct

Name: cbiF [H]

Synonym: lwe1154

Alternate gene names: 116872570

Gene position: 1163421-1164170 (Clockwise)

Preceding gene: 116872569

Following gene: 116872571

Centisome position: 41.34

GC content: 39.87

Gene sequence:

>750_bases
ATGGCAGAAGTACATTTCGTCGGAGCAGGACCAGGAGATAAAGAATTAATCACACTAAAAGGATATCAACTATTAAAAGA
AGCAGATGTTGTAATTTATGCAGGGTCACTCGTGAATCCAGAGTTATTGGACTATTGTAAAGCAGGTTCAGAAATCCATA
ATAGTGCGACAATGAACTTAATTGAAATTATCGATTGTATGGAAGCAGGTGTTACGGCTGGAAAAGAAGTGGTTCGTCTG
CAAACAGGGGATTTCTCTATTTATGGTTCGATTCGTGAACAAGTCGAGGAAATGAAAAAACGAGGTATTCCTTTCACTTG
TACGCCAGGCGTGAGTTCATTCCTTGGCGCGGCAAGTAGCTTTGGTGTGGAGTATACGGTTCCTGAAGTAAGTCAGAGTG
TGATTATTACTAGAATGGCAGGCAGAACTCCAGTACCCGCACGCGAATCACTCAGATCTTATGCTGCACACCAAACTTCC
ATGGTTATTTTCTTATCGGTTCAAGGTATTCGAAAAGTAGTTTCAGAACTAATTAAAGGCGGTTATAAACCTGAAACTAA
AGCAGCGGTTATTTATAAAGCAACTTGGGCAGAAGAAAAGAAAGTAACTGGCACACTAAAAGATATCGCGGAAAAAGTGA
CGGAAGCAGGAATTACGAAAACAGCGCTTATTATGGTCGGCGATTTTCTTGGCGAAGAGTTTTATTATTCCAAGCTATAT
GACAAGGACTTTAAACATGAATATAGATAA

Upstream 100 bases:

>100_bases
TCCAAGTTTCGAGTTGGCATAAACTTGGAGCGGGACACTATTTTGAACCACAAAATCCAACCGTGATTATCGGCTGTAAA
AAACTAGAGGAGTGAAGAAA

Downstream 100 bases:

>100_bases
ACTGGCAATCATTGCCGTTACAGAACGTGGTCGGGATTTAGCAATCACATTGACAAAAAAAATACCGGCAACCATTTTTG
TACCAGAGAAACATCAAAAT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAEVHFVGAGPGDKELITLKGYQLLKEADVVIYAGSLVNPELLDYCKAGSEIHNSATMNLIEIIDCMEAGVTAGKEVVRL
QTGDFSIYGSIREQVEEMKKRGIPFTCTPGVSSFLGAASSFGVEYTVPEVSQSVIITRMAGRTPVPARESLRSYAAHQTS
MVIFLSVQGIRKVVSELIKGGYKPETKAAVIYKATWAEEKKVTGTLKDIAEKVTEAGITKTALIMVGDFLGEEFYYSKLY
DKDFKHEYR

Sequences:

>Translated_249_residues
MAEVHFVGAGPGDKELITLKGYQLLKEADVVIYAGSLVNPELLDYCKAGSEIHNSATMNLIEIIDCMEAGVTAGKEVVRL
QTGDFSIYGSIREQVEEMKKRGIPFTCTPGVSSFLGAASSFGVEYTVPEVSQSVIITRMAGRTPVPARESLRSYAAHQTS
MVIFLSVQGIRKVVSELIKGGYKPETKAAVIYKATWAEEKKVTGTLKDIAEKVTEAGITKTALIMVGDFLGEEFYYSKLY
DKDFKHEYR
>Mature_248_residues
AEVHFVGAGPGDKELITLKGYQLLKEADVVIYAGSLVNPELLDYCKAGSEIHNSATMNLIEIIDCMEAGVTAGKEVVRLQ
TGDFSIYGSIREQVEEMKKRGIPFTCTPGVSSFLGAASSFGVEYTVPEVSQSVIITRMAGRTPVPARESLRSYAAHQTSM
VIFLSVQGIRKVVSELIKGGYKPETKAAVIYKATWAEEKKVTGTLKDIAEKVTEAGITKTALIMVGDFLGEEFYYSKLYD
KDFKHEYR

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=28.5106382978723, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=24.0343347639485, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27314; Mature: 27183

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVHFVGAGPGDKELITLKGYQLLKEADVVIYAGSLVNPELLDYCKAGSEIHNSATMNL
CCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHH
IEIIDCMEAGVTAGKEVVRLQTGDFSIYGSIREQVEEMKKRGIPFTCTPGVSSFLGAASS
HHHHHHHHHCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
FGVEYTVPEVSQSVIITRMAGRTPVPARESLRSYAAHQTSMVIFLSVQGIRKVVSELIKG
CCCEEECCHHHHHEEEEHHCCCCCCCHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHC
GYKPETKAAVIYKATWAEEKKVTGTLKDIAEKVTEAGITKTALIMVGDFLGEEFYYSKLY
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DKDFKHEYR
CCCCCCCCC
>Mature Secondary Structure 
AEVHFVGAGPGDKELITLKGYQLLKEADVVIYAGSLVNPELLDYCKAGSEIHNSATMNL
CEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHH
IEIIDCMEAGVTAGKEVVRLQTGDFSIYGSIREQVEEMKKRGIPFTCTPGVSSFLGAASS
HHHHHHHHHCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
FGVEYTVPEVSQSVIITRMAGRTPVPARESLRSYAAHQTSMVIFLSVQGIRKVVSELIKG
CCCEEECCHHHHHEEEEHHCCCCCCCHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHC
GYKPETKAAVIYKATWAEEKKVTGTLKDIAEKVTEAGITKTALIMVGDFLGEEFYYSKLY
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DKDFKHEYR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]