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The map label for this gene is cobS

Identifier: 116872522

GI number: 116872522

Start: 1121463

End: 1122209

Strand: Direct

Name: cobS

Synonym: lwe1106

Alternate gene names: 116872522

Gene position: 1121463-1122209 (Clockwise)

Preceding gene: 116872521

Following gene: 116872523

Centisome position: 39.85

GC content: 37.88

Gene sequence:

>747_bases
ATGAAAACATTAATTTTACTTATTCAGTTTTTTACACGAATTCCGATACCAATTGCGATTAATATGGATGAGGTTAACTT
GAAAAAAGGGAGCGCTTTACTTCCCTTTGTAGGCCTGATTATTGGTGCCTGGAATTGGCTTGTTTTTACTTTAGTAGCAC
TTGTTATGCCTTTACCTGTTGCGATTATTGCAGGGCTTTTTGCGGAAATTATTATCACTGGTGGATTTCATGTGGATGCA
CTCGCAGATACTGCAGATGGGCTATTTTCATCACGTAAAAAAGAGCGGATGCTTGAAATTATGAAAGATAGTCGAGTTGG
GGCAAACGGCGTGATTGCGATTTGTTTTTATTTTCTTTTTTATGGTGCTTTATTTCTTTCAGTTCCAAGTGCATATGAAA
TTGGTTGGTTATTTTTTGTGTTACCCATTGTGGCAAAGGGTGTAACCATGTTACTTTTTGCAAAAATGACGTATGCAGGT
TCAAATGAAGGACTTGGTTCTATTTTTCTTGGGGTTCCTTGGTGGCCAATCGCGATTTCACAATTGTTTGTTCTCTTTAT
TTTAGGACTAGTTTTTTCTTATATTGGATTGCTTGCTTATGCTGGTGTCATTCTATTCACGATTATTTATCGGACATTTG
TGTATAAACGAATTGGTGGCATGAATGGAGATACGCTCGGTGCTGGTGGACAGATGGGGCAACTGGTGTGTTTATTTTGT
CTAGTGCTAGTTTGGGGGTTGATTTAA

Upstream 100 bases:

>100_bases
GTTTTCAGAGATATTATTGGTCGAGTAAACCAGCAAATTGCGAACCAGGTAGATGAAGTCTATTTTGTAGTAAGTGGCAT
ACCAAAAAGGTGGAAGTAGT

Downstream 100 bases:

>100_bases
GTGCGACTTATTTTTGTGCGGCACGGAGAGACAGACTTGAATTTAGCTAGAAAATATTGTGGACAACTAGATGTTGGTTT
AAATGAACACGGGGTTCAGC

Product: cobalamin (5'-phosphatase) synthetase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKTLILLIQFFTRIPIPIAINMDEVNLKKGSALLPFVGLIIGAWNWLVFTLVALVMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKKERMLEIMKDSRVGANGVIAICFYFLFYGALFLSVPSAYEIGWLFFVLPIVAKGVTMLLFAKMTYAG
SNEGLGSIFLGVPWWPIAISQLFVLFILGLVFSYIGLLAYAGVILFTIIYRTFVYKRIGGMNGDTLGAGGQMGQLVCLFC
LVLVWGLI

Sequences:

>Translated_248_residues
MKTLILLIQFFTRIPIPIAINMDEVNLKKGSALLPFVGLIIGAWNWLVFTLVALVMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKKERMLEIMKDSRVGANGVIAICFYFLFYGALFLSVPSAYEIGWLFFVLPIVAKGVTMLLFAKMTYAG
SNEGLGSIFLGVPWWPIAISQLFVLFILGLVFSYIGLLAYAGVILFTIIYRTFVYKRIGGMNGDTLGAGGQMGQLVCLFC
LVLVWGLI
>Mature_248_residues
MKTLILLIQFFTRIPIPIAINMDEVNLKKGSALLPFVGLIIGAWNWLVFTLVALVMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKKERMLEIMKDSRVGANGVIAICFYFLFYGALFLSVPSAYEIGWLFFVLPIVAKGVTMLLFAKMTYAG
SNEGLGSIFLGVPWWPIAISQLFVLFILGLVFSYIGLLAYAGVILFTIIYRTFVYKRIGGMNGDTLGAGGQMGQLVCLFC
LVLVWGLI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=34.1463414634146, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_LISW6 (A0AHP2)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_849303.1
- STRING:   A0AHP2
- GeneID:   4465046
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe1106
- NMPDR:   fig|386043.6.peg.1075
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   VGPFAVF
- PhylomeDB:   A0AHP2
- ProtClustDB:   CLSK809351
- BioCyc:   LWEL386043:LWE1106-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27138; Mature: 27138

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a1963e0)-; HASH(0x1acfd8e4)-; HASH(0x1acea020)-; HASH(0x185a8788)-; HASH(0x19ecb108)-; HASH(0x1acfd944)-; HASH(0x1acea96c)-; HASH(0x1ace9114)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCH
>Mature Secondary Structure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HHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA