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The map label for this gene is ispD [H]

Identifier: 116872477

GI number: 116872477

Start: 1082577

End: 1083290

Strand: Direct

Name: ispD [H]

Synonym: lwe1061

Alternate gene names: 116872477

Gene position: 1082577-1083290 (Clockwise)

Preceding gene: 116872476

Following gene: 116872478

Centisome position: 38.47

GC content: 33.33

Gene sequence:

>714_bases
ATGATATACGCTCAAATTTTAGCTGGAGGAAAAGGCACGCGAATGGGAAATGTTAGCATGCCAAAACAATTTCTACCTCT
AAATGGTAAACCGATTATTGTTCATACGGTAGAAAAATTTATTTTAAACACACGTTTTGATAAAATTATTATTTCTTCTC
CAAAAGAATGGATGAATCATGCGGAGGATAATATTAAAAAATATATTTCTGATGACCGCATTGTAGTAATCGAAGGTGGG
GAAGACCGTAACGAAACAATTATGAATGGCATTCGTTTTGTAGAAAAAACGTATGGCTTAAATGATGATGATGTAATTAT
TACTCATGATGCAGTTCGTCCGTTTTTAACACATCGAATTATTGAAGAAAATATAGAAGCTGCACTTGAAACTGGAGCAG
TTGATACTGTCATTGAAGCGCTTGATACAATTGTTGAATCAAGCAACCACGACTTTATCACGGATATTCCAGTGAGAGAT
CATATGTATCAAGGACAAACACCCCAAAGTTTTAATATGAAAAAAGTTTATAATCATTATCAAAACTTGACTACTGAAAA
GAAACAAATTTTAACAGATGCTTGTAAGATTTGTTTACTTGCTGGTGATCATGTGAAGCTTGTTAAGGGTGAAATATTCA
ATATTAAGATTACGACACCTTATGACTTAAAAGTGGCTAATGCGATTATTCAGGAGCGGATAGCCAATGATTAA

Upstream 100 bases:

>100_bases
TCTGCAGAACGAATTGTGAAAGAACTTATTAAGTAAGATTTAACACTGGAATTGTGGTATAATAAACACTGTTAAAAAAA
CAGTTAAAGGAGATTAGAGG

Downstream 100 bases:

>100_bases
TCAAGTATATAGACTTGTGTCAGAAAGACAGTTTGAAGTTGCCAATGTGGATGAAAATTTGACGGAAGATGTAGTGGTTG
TTAGACCAACTCATCTATCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2; MEP cytidylyltransferase 2; MCT 2 [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKIIISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLNDDDVIITHDAVRPFLTHRIIEENIEAALETGAVDTVIEALDTIVESSNHDFITDIPVRD
HMYQGQTPQSFNMKKVYNHYQNLTTEKKQILTDACKICLLAGDHVKLVKGEIFNIKITTPYDLKVANAIIQERIAND

Sequences:

>Translated_237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKIIISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLNDDDVIITHDAVRPFLTHRIIEENIEAALETGAVDTVIEALDTIVESSNHDFITDIPVRD
HMYQGQTPQSFNMKKVYNHYQNLTTEKKQILTDACKICLLAGDHVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
>Mature_237_residues
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKIIISSPKEWMNHAEDNIKKYISDDRIVVIEGG
EDRNETIMNGIRFVEKTYGLNDDDVIITHDAVRPFLTHRIIEENIEAALETGAVDTVIEALDTIVESSNHDFITDIPVRD
HMYQGQTPQSFNMKKVYNHYQNLTTEKKQILTDACKICLLAGDHVKLVKGEIFNIKITTPYDLKVANAIIQERIAND

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=30, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI157671913, Length=141, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=26.6375545851528, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26896; Mature: 26896

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKIIISSPKEWMNH
CEEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHCCCCEEEECCCHHHHHH
AEDNIKKYISDDRIVVIEGGEDRNETIMNGIRFVEKTYGLNDDDVIITHDAVRPFLTHRI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
IEENIEAALETGAVDTVIEALDTIVESSNHDFITDIPVRDHMYQGQTPQSFNMKKVYNHY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCCCCCHHHHHHHH
QNLTTEKKQILTDACKICLLAGDHVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
HHCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYAQILAGGKGTRMGNVSMPKQFLPLNGKPIIVHTVEKFILNTRFDKIIISSPKEWMNH
CEEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHCCCCEEEECCCHHHHHH
AEDNIKKYISDDRIVVIEGGEDRNETIMNGIRFVEKTYGLNDDDVIITHDAVRPFLTHRI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
IEENIEAALETGAVDTVIEALDTIVESSNHDFITDIPVRDHMYQGQTPQSFNMKKVYNHY
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCCCCCHHHHHHHH
QNLTTEKKQILTDACKICLLAGDHVKLVKGEIFNIKITTPYDLKVANAIIQERIAND
HHCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]