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The map label for this gene is lplA [H]

Identifier: 116872145

GI number: 116872145

Start: 748892

End: 749881

Strand: Direct

Name: lplA [H]

Synonym: lwe0725

Alternate gene names: 116872145

Gene position: 748892-749881 (Clockwise)

Preceding gene: 116872144

Following gene: 116872146

Centisome position: 26.61

GC content: 35.45

Gene sequence:

>990_bases
GTGATTTATTTAGATAATGAAGATGTGCTTGATCAAGCATACAATTTTGCTATGGAAGAATATGCGCTCCGTTTTTTAGA
TGAGAATGAAACATATTTCATGTTTTACCGGATGAAGCCAACAATAATTGTAGGTAAAAATCAAAACACATTAGAAGAAA
TTAATCATACTTTTGTAGAAAAACATCATGTTCATGTTTTACGCCGGCTGTCGGGCGGTGGAGCAGTTTATAATGATGAA
GGTAATATTAGTTTTAGTATGATAACAAAAGATGATGGGAATAGCTTTCAAAATTTTGCAAGATTTACAGAACCAGTTAT
TCAAGCACTCCAAAAGCTAGGCGTAAATGCAAAACTTAGCGGAAGAAATGATATTGAAGTGGATGGCAAAAAAATAAGCG
GCAATGCACAGTTTGCGACGAATGGCCGATTATATAGTCATGGAACGTTGCTTTTCGATGTTGATTTGTCGATGCTTGAA
GCGGCTCTGCAAGTAGATCCTGAAAAGTATTTATCCAAAGGGGTTAAATCTGTTCGGAGTCGTGTCACCACGATTCGGGA
ACATTTAACGGAAGATATGGATATCCAGGCTTTTAAACAAATTCTCCTAGAGTCTATTTTCGGAACAGCGGATATTCCAC
GTTATATTTTTACAGAAGAAGATAAATTAGCTATTGAAAAAATACGCGAGGAACGCTACTTGAACTGGGATTGGACATAT
GGTAAATCTCCAAAAGCCACTATCCAACGTAAAAAAAGATTCCCAGCTGGAACTATCGAATTTCAGCTGACTTTACACAA
AGGCCAAGTAAAAGAAGCAATCATTTATGGTGATTTTTTTGGAACAAAAGATGTTGCTGATTTGGCTAACAAAATGCTTG
GTTGTCGTTTTGAACGTAAAGCCATCCAAAATGCTTGGCAAGAAATAAATACAAAAGATTATTTTGGTGGAATTGAAAAA
GAAGCTATTCTTGATATGTTATTTGAATAA

Upstream 100 bases:

>100_bases
TTAGCCGAGGCTTAGGAAATGTAACCAAACTTCCACGTCTATTTAATGATCCTGAAATCATTCGGATTACCCTTAAAGCA
AAAGGAGATGCTAGTGGAAA

Downstream 100 bases:

>100_bases
GGATTTAAACTCTGAGATATAGATTCTCAGAGTTTTTTTGTGTAAAAAGAAAACGATTTCATTTTTATATTGACAGTGAT
TAAATATATATAGTATTATT

Product: lipoate-protein ligase family protein

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIYLDNEDVLDQAYNFAMEEYALRFLDENETYFMFYRMKPTIIVGKNQNTLEEINHTFVEKHHVHVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFARFTEPVIQALQKLGVNAKLSGRNDIEVDGKKISGNAQFATNGRLYSHGTLLFDVDLSMLE
AALQVDPEKYLSKGVKSVRSRVTTIREHLTEDMDIQAFKQILLESIFGTADIPRYIFTEEDKLAIEKIREERYLNWDWTY
GKSPKATIQRKKRFPAGTIEFQLTLHKGQVKEAIIYGDFFGTKDVADLANKMLGCRFERKAIQNAWQEINTKDYFGGIEK
EAILDMLFE

Sequences:

>Translated_329_residues
MIYLDNEDVLDQAYNFAMEEYALRFLDENETYFMFYRMKPTIIVGKNQNTLEEINHTFVEKHHVHVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFARFTEPVIQALQKLGVNAKLSGRNDIEVDGKKISGNAQFATNGRLYSHGTLLFDVDLSMLE
AALQVDPEKYLSKGVKSVRSRVTTIREHLTEDMDIQAFKQILLESIFGTADIPRYIFTEEDKLAIEKIREERYLNWDWTY
GKSPKATIQRKKRFPAGTIEFQLTLHKGQVKEAIIYGDFFGTKDVADLANKMLGCRFERKAIQNAWQEINTKDYFGGIEK
EAILDMLFE
>Mature_329_residues
MIYLDNEDVLDQAYNFAMEEYALRFLDENETYFMFYRMKPTIIVGKNQNTLEEINHTFVEKHHVHVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFARFTEPVIQALQKLGVNAKLSGRNDIEVDGKKISGNAQFATNGRLYSHGTLLFDVDLSMLE
AALQVDPEKYLSKGVKSVRSRVTTIREHLTEDMDIQAFKQILLESIFGTADIPRYIFTEEDKLAIEKIREERYLNWDWTY
GKSPKATIQRKKRFPAGTIEFQLTLHKGQVKEAIIYGDFFGTKDVADLANKMLGCRFERKAIQNAWQEINTKDYFGGIEK
EAILDMLFE

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21729884, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21729882, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21729880, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21729878, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1790846, Length=315, Percent_Identity=31.4285714285714, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71981909, Length=250, Percent_Identity=28.8, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322415, Length=259, Percent_Identity=28.957528957529, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28573464, Length=258, Percent_Identity=27.906976744186, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37926; Mature: 37926

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNEDVLDQAYNFAMEEYALRFLDENETYFMFYRMKPTIIVGKNQNTLEEINHTFVE
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEECCCCHHHHHHHHHHH
KHHVHVLRRLSGGGAVYNDEGNISFSMITKDDGNSFQNFARFTEPVIQALQKLGVNAKLS
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDIEVDGKKISGNAQFATNGRLYSHGTLLFDVDLSMLEAALQVDPEKYLSKGVKSVRS
CCCCCEECCEEECCCCEEEECCEEEECCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RVTTIREHLTEDMDIQAFKQILLESIFGTADIPRYIFTEEDKLAIEKIREERYLNWDWTY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHEECCCHHHHHHHHHHHHCCCCCCCC
GKSPKATIQRKKRFPAGTIEFQLTLHKGQVKEAIIYGDFFGTKDVADLANKMLGCRFERK
CCCCHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHH
AIQNAWQEINTKDYFGGIEKEAILDMLFE
HHHHHHHHCCCHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIYLDNEDVLDQAYNFAMEEYALRFLDENETYFMFYRMKPTIIVGKNQNTLEEINHTFVE
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEECCCCHHHHHHHHHHH
KHHVHVLRRLSGGGAVYNDEGNISFSMITKDDGNSFQNFARFTEPVIQALQKLGVNAKLS
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDIEVDGKKISGNAQFATNGRLYSHGTLLFDVDLSMLEAALQVDPEKYLSKGVKSVRS
CCCCCEECCEEECCCCEEEECCEEEECCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RVTTIREHLTEDMDIQAFKQILLESIFGTADIPRYIFTEEDKLAIEKIREERYLNWDWTY
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHEECCCHHHHHHHHHHHHCCCCCCCC
GKSPKATIQRKKRFPAGTIEFQLTLHKGQVKEAIIYGDFFGTKDVADLANKMLGCRFERK
CCCCHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHH
AIQNAWQEINTKDYFGGIEKEAILDMLFE
HHHHHHHHCCCHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]