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The map label for this gene is frwB [H]

Identifier: 116872020

GI number: 116872020

Start: 636722

End: 637030

Strand: Direct

Name: frwB [H]

Synonym: lwe0600

Alternate gene names: 116872020

Gene position: 636722-637030 (Clockwise)

Preceding gene: 116872019

Following gene: 116872021

Centisome position: 22.63

GC content: 35.6

Gene sequence:

>309_bases
ATGAAAATTGTAGCAGTAACTTCCTGCCCAAGCGGTGTGGCGCATACGTATATGTCGGCAGAATCACTTGAACTCTCCGC
TAAAAAAATGGGTGTCACGATTAAAGTAGAAACGCAAGGCTCCTCTGGCATCGAAAATAAATTAACTGTTAAAGAAATCG
AAGAAGCGGATTGCGTCATTATCACAAATGATGTCGAAATAAGAGAAAGCCAACGATTTAAAGGAAAAAAAGTGGTTAAA
ATGAGCGTTTCAGAAATCATAAAAAAATCAGATACGTTAATTAAAAAATTACAAACAATGTTCGAATAA

Upstream 100 bases:

>100_bases
TGAAGAAAGATGCCACAGAGCTTGACGAGGCACTGGAAGAAGGGCAAGACATCGATGATTTAGATATTAATTTTGAAGAT
ATTTAGAAAGGATGATAATA

Downstream 100 bases:

>100_bases
AATTTTAGGAGGAATAATTAATTGAAATTACCAATTAAAAAAGCAGTAGAAGCACTTTTGAAATTACAAACAACCGGTGA
TAGCGCAACTCTTATTGGGG

Product: putative PTS system fructose-like transporter subunit EIIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 102; Mature: 102

Protein sequence:

>102_residues
MKIVAVTSCPSGVAHTYMSAESLELSAKKMGVTIKVETQGSSGIENKLTVKEIEEADCVIITNDVEIRESQRFKGKKVVK
MSVSEIIKKSDTLIKKLQTMFE

Sequences:

>Translated_102_residues
MKIVAVTSCPSGVAHTYMSAESLELSAKKMGVTIKVETQGSSGIENKLTVKEIEEADCVIITNDVEIRESQRFKGKKVVK
MSVSEIIKKSDTLIKKLQTMFE
>Mature_102_residues
MKIVAVTSCPSGVAHTYMSAESLELSAKKMGVTIKVETQGSSGIENKLTVKEIEEADCVIITNDVEIRESQRFKGKKVVK
MSVSEIIKKSDTLIKKLQTMFE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=97, Percent_Identity=51.5463917525773, Blast_Score=115, Evalue=4e-28,
Organism=Escherichia coli, GI87082348, Length=101, Percent_Identity=48.5148514851485, Blast_Score=102, Evalue=4e-24,
Organism=Escherichia coli, GI1786951, Length=98, Percent_Identity=43.8775510204082, Blast_Score=97, Evalue=3e-22,
Organism=Escherichia coli, GI1788730, Length=99, Percent_Identity=37.3737373737374, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1788492, Length=95, Percent_Identity=44.2105263157895, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1790390, Length=89, Percent_Identity=42.6966292134831, Blast_Score=75, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11252; Mature: 11252

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVAVTSCPSGVAHTYMSAESLELSAKKMGVTIKVETQGSSGIENKLTVKEIEEADCVI
CEEEEEECCCCCHHHHHCCCCHHEEEHHHCCEEEEEEECCCCCCCCCEEHEECCCCCEEE
ITNDVEIRESQRFKGKKVVKMSVSEIIKKSDTLIKKLQTMFE
EECCCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVAVTSCPSGVAHTYMSAESLELSAKKMGVTIKVETQGSSGIENKLTVKEIEEADCVI
CEEEEEECCCCCHHHHHCCCCHHEEEHHHCCEEEEEEECCCCCCCCCEEHEECCCCCEEE
ITNDVEIRESQRFKGKKVVKMSVSEIIKKSDTLIKKLQTMFE
EECCCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]