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The map label for this gene is phrB [H]

Identifier: 116871975

GI number: 116871975

Start: 594096

End: 595508

Strand: Direct

Name: phrB [H]

Synonym: lwe0555

Alternate gene names: 116871975

Gene position: 594096-595508 (Clockwise)

Preceding gene: 116871974

Following gene: 116871976

Centisome position: 21.11

GC content: 34.82

Gene sequence:

>1413_bases
ATGACTTCAGTAATGTGGTTTCGCAGAGATCTTCGAATAAACGATAATAAAGCCCTCTATCATGCTAGTAAAGAGAATGA
TTTAATTTTGTTGTTTCAAGTTAACCCAGAACAATTTATTTCAGGAAGTCCTAGTCATCAAGCATTTTTTTCGAGCGTGG
CTAATTTTAAAAAAGAAATAGATAAAAATGCTCATTTACAAATTATGTTTGGCGAACCAATTGAACTTTTGAAACGACTG
AAAGATGCGATTCCAGCATGGGATAAAGTGTTTTTCAATCAAGATGAAACAGGATACGGAGCAAAAAGAGATAAAGCAGC
ACGCGCATTTTTTGAATATAACAATATTACTGTTCATGCTTATCATGATGCCTATCTTCATTCAGCCGAAGAAGTAAAAA
AAACATCCACAGAATACTATAAAATTTTCACTCCTTATTATAAAAAATGGCGTGAGGAAATAAAAGAAACGCCATTTAAG
TCTGTTTTAACGCCAGAAAATATTATAAAAAAAGAGTTATTTCCTCATGACGAAGAACAGTTTGAAAAATTAATTCAAGA
TTTACCTGAACTAGATTTGAAAGTGTTAGGAGAAGAGGCAGCAAATACTCGGTTAAAAAATTTTATAAAGAGAGATTTAG
CAGAATATGCCAAAGCGAGAGATATCCCAGACTCGGATCAAACTAGTCATTTATCACGTTATTTACGCACGGGTGAAATT
TCGATTAGAACCGTTTGGCAAGCATTGCAACAAACAAAACCCACAGAAGGCCGAGCAATATTTGAAAAAGAGTTATGTTG
GCGCGACTTTTATAACATGATTTATGTTTCTTTCCCAAATCAAAAAAACGAACCAATTCAAGAAAATTATCGTTTTATTG
AGTGGGAAAATAATCGCGAGTTTTTCAAACAATGGCAAGAAGGTAAAACGGGTTATCCTCTCGTAGATGCGGCTATGCGT
CAGTTGAAAGAAACGGGCTGGATGCATAACAGGCTAAGAATGATTACGGCTTCCTTTTTGACTAAAGATTTACTGATTGA
TTGGCGTTATGGCGAGAAATATTTCCAACAAATGTTGATTGATTATGATCCAGCAAGTAATATCAGTGGTTGGCAATGGG
CTGCATCAACCGGAACAGACGCAGTACCTTATTTTAGAATTTTTAACCCAACGACACAATCAGAAAAGTTTGATCCTTCT
GGGAAATTTATTCGTAAATATGTGGAAGAACTATCGAATTTGCCTGATAAATTTATCCATCAACCTGAAAAAATGTCTGA
AAGCCAGCAAAAAGAGCATAATTTAATTTTAGGAGACGATTATCCTTTGCCAATTGTTGACCATAAAGAGCGCCGAAAAT
TAGCAATTTCGCGTTATGAGTTTAGCAAAGAGCAGTTCAGGGGAAATATATAA

Upstream 100 bases:

>100_bases
AAAAGTTAACAGATTTTTTTGTGTTATAAGCCCTTTGCTTTTATAAATTGCTAAGCGTTTCGTATAATAAGCATAAGTAA
TTCGCGAAAGGTGGGAAAGA

Downstream 100 bases:

>100_bases
ATAATTGAGTCAATCATTTACTTAGTTAGATGGTTGATTTTTTGTGGAAGAGTTTATAAGAGTGAGTTTGAGCAACCTTC
TAGGAGTAATAACTTGGGTA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTSVMWFRRDLRINDNKALYHASKENDLILLFQVNPEQFISGSPSHQAFFSSVANFKKEIDKNAHLQIMFGEPIELLKRL
KDAIPAWDKVFFNQDETGYGAKRDKAARAFFEYNNITVHAYHDAYLHSAEEVKKTSTEYYKIFTPYYKKWREEIKETPFK
SVLTPENIIKKELFPHDEEQFEKLIQDLPELDLKVLGEEAANTRLKNFIKRDLAEYAKARDIPDSDQTSHLSRYLRTGEI
SIRTVWQALQQTKPTEGRAIFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREFFKQWQEGKTGYPLVDAAMR
QLKETGWMHNRLRMITASFLTKDLLIDWRYGEKYFQQMLIDYDPASNISGWQWAASTGTDAVPYFRIFNPTTQSEKFDPS
GKFIRKYVEELSNLPDKFIHQPEKMSESQQKEHNLILGDDYPLPIVDHKERRKLAISRYEFSKEQFRGNI

Sequences:

>Translated_470_residues
MTSVMWFRRDLRINDNKALYHASKENDLILLFQVNPEQFISGSPSHQAFFSSVANFKKEIDKNAHLQIMFGEPIELLKRL
KDAIPAWDKVFFNQDETGYGAKRDKAARAFFEYNNITVHAYHDAYLHSAEEVKKTSTEYYKIFTPYYKKWREEIKETPFK
SVLTPENIIKKELFPHDEEQFEKLIQDLPELDLKVLGEEAANTRLKNFIKRDLAEYAKARDIPDSDQTSHLSRYLRTGEI
SIRTVWQALQQTKPTEGRAIFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREFFKQWQEGKTGYPLVDAAMR
QLKETGWMHNRLRMITASFLTKDLLIDWRYGEKYFQQMLIDYDPASNISGWQWAASTGTDAVPYFRIFNPTTQSEKFDPS
GKFIRKYVEELSNLPDKFIHQPEKMSESQQKEHNLILGDDYPLPIVDHKERRKLAISRYEFSKEQFRGNI
>Mature_469_residues
TSVMWFRRDLRINDNKALYHASKENDLILLFQVNPEQFISGSPSHQAFFSSVANFKKEIDKNAHLQIMFGEPIELLKRLK
DAIPAWDKVFFNQDETGYGAKRDKAARAFFEYNNITVHAYHDAYLHSAEEVKKTSTEYYKIFTPYYKKWREEIKETPFKS
VLTPENIIKKELFPHDEEQFEKLIQDLPELDLKVLGEEAANTRLKNFIKRDLAEYAKARDIPDSDQTSHLSRYLRTGEIS
IRTVWQALQQTKPTEGRAIFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREFFKQWQEGKTGYPLVDAAMRQ
LKETGWMHNRLRMITASFLTKDLLIDWRYGEKYFQQMLIDYDPASNISGWQWAASTGTDAVPYFRIFNPTTQSEKFDPSG
KFIRKYVEELSNLPDKFIHQPEKMSESQQKEHNLILGDDYPLPIVDHKERRKLAISRYEFSKEQFRGNI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=28.0487804878049, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4758072, Length=497, Percent_Identity=28.7726358148893, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI188536103, Length=426, Percent_Identity=28.4037558685446, Blast_Score=155, Evalue=9e-38,
Organism=Escherichia coli, GI1786926, Length=479, Percent_Identity=37.9958246346555, Blast_Score=300, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6324962, Length=511, Percent_Identity=31.1154598825832, Blast_Score=216, Evalue=8e-57,
Organism=Drosophila melanogaster, GI17137248, Length=491, Percent_Identity=29.5315682281059, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24585455, Length=491, Percent_Identity=29.5315682281059, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24648152, Length=519, Percent_Identity=24.8554913294798, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55891; Mature: 55760

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVMWFRRDLRINDNKALYHASKENDLILLFQVNPEQFISGSPSHQAFFSSVANFKKEI
CCCCHHHHHCCEECCCCEEEEECCCCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHH
DKNAHLQIMFGEPIELLKRLKDAIPAWDKVFFNQDETGYGAKRDKAARAFFEYNNITVHA
CCCCCEEEEECCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHEEECCEEEEE
YHDAYLHSAEEVKKTSTEYYKIFTPYYKKWREEIKETPFKSVLTPENIIKKELFPHDEEQ
EHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCHHH
FEKLIQDLPELDLKVLGEEAANTRLKNFIKRDLAEYAKARDIPDSDQTSHLSRYLRTGEI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
SIRTVWQALQQTKPTEGRAIFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNRE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCEEEEECCCHH
FFKQWQEGKTGYPLVDAAMRQLKETGWMHNRLRMITASFLTKDLLIDWRYGEKYFQQMLI
HHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
DYDPASNISGWQWAASTGTDAVPYFRIFNPTTQSEKFDPSGKFIRKYVEELSNLPDKFIH
CCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCC
QPEKMSESQQKEHNLILGDDYPLPIVDHKERRKLAISRYEFSKEQFRGNI
CCHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSVMWFRRDLRINDNKALYHASKENDLILLFQVNPEQFISGSPSHQAFFSSVANFKKEI
CCCHHHHHCCEECCCCEEEEECCCCCEEEEEEECHHHHHCCCCCHHHHHHHHHHHHHHH
DKNAHLQIMFGEPIELLKRLKDAIPAWDKVFFNQDETGYGAKRDKAARAFFEYNNITVHA
CCCCCEEEEECCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHEEECCEEEEE
YHDAYLHSAEEVKKTSTEYYKIFTPYYKKWREEIKETPFKSVLTPENIIKKELFPHDEEQ
EHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCHHH
FEKLIQDLPELDLKVLGEEAANTRLKNFIKRDLAEYAKARDIPDSDQTSHLSRYLRTGEI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
SIRTVWQALQQTKPTEGRAIFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNRE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCEEEEECCCHH
FFKQWQEGKTGYPLVDAAMRQLKETGWMHNRLRMITASFLTKDLLIDWRYGEKYFQQMLI
HHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
DYDPASNISGWQWAASTGTDAVPYFRIFNPTTQSEKFDPSGKFIRKYVEELSNLPDKFIH
CCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCC
QPEKMSESQQKEHNLILGDDYPLPIVDHKERRKLAISRYEFSKEQFRGNI
CCHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]