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The map label for this gene is lmrB [H]

Identifier: 116871895

GI number: 116871895

Start: 507376

End: 508791

Strand: Direct

Name: lmrB [H]

Synonym: lwe0475

Alternate gene names: 116871895

Gene position: 507376-508791 (Clockwise)

Preceding gene: 116871892

Following gene: 116871896

Centisome position: 18.03

GC content: 41.1

Gene sequence:

>1416_bases
ATGCAACAAGAAGCAACAGGTGGGCAGAAGATTCGTCCGATACCGATTATTGCCTCGTTTTTGATGGCAGGTTTTATTGG
ACTATTTAGTGAAACTGCTCTTAACATGGCGCTTAGTGATTTGATTCAGGTGTTTGATATTAGTTCAGCGACAGTACAGT
GGCTTACGACAGGCTATTTGCTAACGCTTGGAATATTAGTACCGATTTCGGGATTACTTTTACAATGGTTTACGACACGA
GGTTTATTTTTTACAGCAGTGAGTTTTTCGATTGCTGGTACGCTTATTGCGGCACTTTCGCCAACGTTTGCGATGTTAAT
GATTGGACGTGTAGTGCAAGCAGTAGGTACGGCGCTATTACTACCGTTAATGTTTAACACAATTTTACTGATTTTCCCAG
AGCATAAACGTGGCTCAGCAATGGGAATGATTGGTCTGGTAATTATGTTTGCGCCAGCAGTTGGTCCAACGATTTCAGGA
CTTATTTTAGAAAACTTAACTTGGAACTGGATTTTCTGGATTTCCTTGCCATTCTTAATTATTGCACTATTATTCGGAAT
GAAATTTATGCAAAATGTTTCGGTTGTTACGAAGCCGAAAATTGATATTTTATCGATTATTCTTTCGACGCTAGGTTTTG
GTGGAGTTGTATTTGCCTTTAGTAGTGCGGGAGAAAGTGGCTGGGGAAGCGCGACAGTGTTAGTTTCTATCATTGGTGGT
GGCATTGCACTTGGACTCTTTGTTTGGCGCCAACTAACGATGGAAAAACCTTTAATGGACTTGAAAGTATTTAAATACCC
AATGTTCACGTTAGGACTTATTTTAGTATTTATCAGTTTTATGATGATTCTGTCGACGATGATTTTACTACCGCTATACT
TGCAAAATAGTTTAGCACTCGCGGCATTTTCTGCGGGATTAGTGCTACTCCCAGGCGGGGTGCTGAATGGTTTAATGTCA
CCATTTACTGGGCGTTTGTTTGATGCATACGGTCCACGCGCACTTGTTATTCCAGGATTTATCGTAGCAGTTGTGGCGCT
ATTTTTCTTAACAAGAATAGAAGTTGGCACATCTGCACTAACAATTATCGTGCTACATTCCGTGTTAATGATTGGGATTT
CGATGGTCATGATGCCAGCACAAACGAACGGGTTAAACCAATTACCACGAAATTTATATCCTGATGGCACGGCGATTATG
AACACATTGCAACAAGTTTCCGGTGCGATTGGAACAGCTGTTGCGATTACGATCATGTCTGCTGGACAAAAAGCTTATAT
GGAAACGGCACAAGGAATGGGACCAGAGCAAATGGTTGCTTCACTGACAGCAGGAATCCAAAATGCCTTCGTCTTTGGAC
TAATTATGGCTTGTATCGGACTTCTGTGTTCGCTCTTTATTCGTAAAGCTAAATAA

Upstream 100 bases:

>100_bases
TTTTTCATGAAAATAAAGCTTGAAACGTTGACTTATTGGCGAAATCTCCGTAATATCAAACGTAGACTACTAAATTTTAG
TCTGAAAAGGGGGAAATTAT

Downstream 100 bases:

>100_bases
TAAAGCGAAGAGAACTTCTCGTAATGAGAGGTTCTTTTTTGTTTTGACAAATAGGTTCTGAGTGGTTAAAATGATATTAA
ATATAGTTTATAAAGTTTGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIGG
GIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPRNLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGMGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK

Sequences:

>Translated_471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIGG
GIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPRNLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGMGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
>Mature_471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIGG
GIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPRNLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGMGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=414, Percent_Identity=26.8115942028986, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081983, Length=381, Percent_Identity=28.6089238845144, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=25.1141552511416, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790195, Length=416, Percent_Identity=26.4423076923077, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=394, Percent_Identity=28.4263959390863, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6325455, Length=246, Percent_Identity=28.4552845528455, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=444, Percent_Identity=23.1981981981982, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50349; Mature: 50349

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTLGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLI
HHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
IALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIGG
HHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
GIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSAL
HHHHCCHHEECCHHHHHHHCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHH
TIIVLHSVLMIGISMVMMPAQTNGLNQLPRNLYPDGTAIMNTLQQVSGAIGTAVAITIMS
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQKAYMETAQGMGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTLGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLI
HHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
IALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIGG
HHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
GIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSAL
HHHHCCHHEECCHHHHHHHCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHH
TIIVLHSVLMIGISMVMMPAQTNGLNQLPRNLYPDGTAIMNTLQQVSGAIGTAVAITIMS
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQKAYMETAQGMGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]