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The map label for this gene is gatA [H]

Identifier: 116871766

GI number: 116871766

Start: 367382

End: 367852

Strand: Direct

Name: gatA [H]

Synonym: lwe0346

Alternate gene names: 116871766

Gene position: 367382-367852 (Clockwise)

Preceding gene: 116871765

Following gene: 116871767

Centisome position: 13.05

GC content: 38.64

Gene sequence:

>471_bases
ATGAAAATGGTGCATGAGGAGTTAGTATTTCCGGGGCTTACCGCTTCTAATTATGAAGAGGTTATTAAGTTAGTTGGAAG
TAAGATGTGTGCGAAAGGATTTGTTAAAGACAGTTATGTGGATGCTGTTTTAGAGCGGGAAAAGGTATTTCCGACAGGCC
TTCAAATAGGGGATTTTGGCATTGCGATTCCGCATACTGACTCTAAACATGTTGAAAACTCGATGGTAGGAATTGCTACA
TTGAAAGATGCTGTAATATTCCAGTCGATGGTGGATCCAAGTACAGAAGTAGCAGCAAAATTAGTTTTTTTACTCGCTGT
AAAAGATCCTGATGGCCAAATGGAAATGCTAAAACAGTTGATGAGCATTTTCCAAAACATCACATTGCTTGAAGCTCTTT
GGCAAGAAAGTAGCCCTCAAAAAATGGCGGAATTATTAATCGATTTGAAACTTACCCCAATCCAAAACTAA

Upstream 100 bases:

>100_bases
CAAGTGATAGACAAATTAAAAAACGCAACTAGCGGGACGGCGATTTGTCAGATATTTAAAGAGAATTTAACAAAATAAAA
AATAGAAAGGAAGTGATGAG

Downstream 100 bases:

>100_bases
GAAAGGAAGAGGAAAATGTATAGTATTTATGAAGCTCCATTTATGAAAGAAATGTGTGAGATGACGCAAAATTTATGGCG
TAATGGCTGGGCAGAAAAAA

Product: PTS system, IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gat; PTS system galactitol-specific EIIA component [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKMVHEELVFPGLTASNYEEVIKLVGSKMCAKGFVKDSYVDAVLEREKVFPTGLQIGDFGIAIPHTDSKHVENSMVGIAT
LKDAVIFQSMVDPSTEVAAKLVFLLAVKDPDGQMEMLKQLMSIFQNITLLEALWQESSPQKMAELLIDLKLTPIQN

Sequences:

>Translated_156_residues
MKMVHEELVFPGLTASNYEEVIKLVGSKMCAKGFVKDSYVDAVLEREKVFPTGLQIGDFGIAIPHTDSKHVENSMVGIAT
LKDAVIFQSMVDPSTEVAAKLVFLLAVKDPDGQMEMLKQLMSIFQNITLLEALWQESSPQKMAELLIDLKLTPIQN
>Mature_156_residues
MKMVHEELVFPGLTASNYEEVIKLVGSKMCAKGFVKDSYVDAVLEREKVFPTGLQIGDFGIAIPHTDSKHVENSMVGIAT
LKDAVIFQSMVDPSTEVAAKLVFLLAVKDPDGQMEMLKQLMSIFQNITLLEALWQESSPQKMAELLIDLKLTPIQN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788410, Length=126, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17298; Mature: 17298

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMVHEELVFPGLTASNYEEVIKLVGSKMCAKGFVKDSYVDAVLEREKVFPTGLQIGDFG
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCE
IAIPHTDSKHVENSMVGIATLKDAVIFQSMVDPSTEVAAKLVFLLAVKDPDGQMEMLKQL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHH
MSIFQNITLLEALWQESSPQKMAELLIDLKLTPIQN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKMVHEELVFPGLTASNYEEVIKLVGSKMCAKGFVKDSYVDAVLEREKVFPTGLQIGDFG
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCE
IAIPHTDSKHVENSMVGIATLKDAVIFQSMVDPSTEVAAKLVFLLAVKDPDGQMEMLKQL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHH
MSIFQNITLLEALWQESSPQKMAELLIDLKLTPIQN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]