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The map label for this gene is thiE

Identifier: 116871709

GI number: 116871709

Start: 314030

End: 314656

Strand: Direct

Name: thiE

Synonym: lwe0289

Alternate gene names: 116871709

Gene position: 314030-314656 (Clockwise)

Preceding gene: 116871708

Following gene: 116871710

Centisome position: 11.16

GC content: 45.77

Gene sequence:

>627_bases
GTGAAAGAAGAATTAGCTGTATATTTTATCGCTGGAACGCAAGACATTGTGCGCGGAACGTTGCCAAGTGTGTTAGAAGA
AGCACTGAAAGGCGGAATCACTTGCTTCCAATACCGTGAAAAAGGAGCAGGCTCACTCCAAACTGCCTCCGAAAGAAAAG
AAATGGCGTTAGAATGCCAAAAATTATGCGCAAAATATCAAGTGCCATTCATTATTAACGATGATGTCGTCCTAGCCCTC
GAAATCGGCGCGGACGGCATCCATGTCGGCCAAAACGACGAGGAAATCCACCAAGTTATCACGAGTTGCGCCGGAAAAAT
GAAAATTGGGCTTTCCGTTCATTCAGTTAGTGAAGCGGAGGAGGCAGAACGACTTGGTGCGGTGGATTATATTGGTGTAG
GACCAATTTTTCCAACGATTTCCAAAGCAGATGCGGAGCCAGAAAGCGGTACAGAGATTTTAAAAGAGATTCGTCGTGCA
GGGATTAAGCTCCCAATCGTTGGTATTGGCGGGATTAATGAAGCAAATATAGCGGAAGTACTTGCGGCAGGTGCTGACGG
CGTATCAGTTATCTCAGCGATTACTCGGGCGGATGATTATCAGTTGGTTATCGCTAATCTAATATGA

Upstream 100 bases:

>100_bases
TTGCGAAAGAGTTTATCACGAGTGCAATTAAATATCCGCTTGGAATTGGTCACGGTCATGGTCCAACCAACCATTTTGCT
TATAGAATGGAGGAAGGTAA

Downstream 100 bases:

>100_bases
GAATTTTAAAAGACAAAAACTATAGATGAATTCTCAACAAATGTTAATTATTAACTTTCTTCTGAAAATTAAATTGTTTT
CCACTTGTTATTTCATTAAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKEELAVYFIAGTQDIVRGTLPSVLEEALKGGITCFQYREKGAGSLQTASERKEMALECQKLCAKYQVPFIINDDVVLAL
EIGADGIHVGQNDEEIHQVITSCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPESGTEILKEIRRA
GIKLPIVGIGGINEANIAEVLAAGADGVSVISAITRADDYQLVIANLI

Sequences:

>Translated_208_residues
MKEELAVYFIAGTQDIVRGTLPSVLEEALKGGITCFQYREKGAGSLQTASERKEMALECQKLCAKYQVPFIINDDVVLAL
EIGADGIHVGQNDEEIHQVITSCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPESGTEILKEIRRA
GIKLPIVGIGGINEANIAEVLAAGADGVSVISAITRADDYQLVIANLI
>Mature_208_residues
MKEELAVYFIAGTQDIVRGTLPSVLEEALKGGITCFQYREKGAGSLQTASERKEMALECQKLCAKYQVPFIINDDVVLAL
EIGADGIHVGQNDEEIHQVITSCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPESGTEILKEIRRA
GIKLPIVGIGGINEANIAEVLAAGADGVSVISAITRADDYQLVIANLI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=191, Percent_Identity=30.3664921465969, Blast_Score=78, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=219, Percent_Identity=37.4429223744292, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LISW6 (A0AFC5)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_848490.1
- ProteinModelPortal:   A0AFC5
- SMR:   A0AFC5
- STRING:   A0AFC5
- GeneID:   4465686
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe0289
- NMPDR:   fig|386043.6.peg.278
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   A0AFC5
- ProtClustDB:   PRK00043
- BioCyc:   LWEL386043:LWE0289-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22067; Mature: 22067

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEELAVYFIAGTQDIVRGTLPSVLEEALKGGITCFQYREKGAGSLQTASERKEMALECQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCHHHHHHHHHHHHHH
KLCAKYQVPFIINDDVVLALEIGADGIHVGQNDEEIHQVITSCAGKMKIGLSVHSVSEAE
HHHHHCCCCEEECCCEEEEEEECCCCEECCCCHHHHHHHHHHHCCCEEECEEEHHHHHHH
EAERLGAVDYIGVGPIFPTISKADAEPESGTEILKEIRRAGIKLPIVGIGGINEANIAEV
HHHHCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
LAAGADGVSVISAITRADDYQLVIANLI
HHCCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MKEELAVYFIAGTQDIVRGTLPSVLEEALKGGITCFQYREKGAGSLQTASERKEMALECQ
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCHHHHHHHHHHHHHH
KLCAKYQVPFIINDDVVLALEIGADGIHVGQNDEEIHQVITSCAGKMKIGLSVHSVSEAE
HHHHHCCCCEEECCCEEEEEEECCCCEECCCCHHHHHHHHHHHCCCEEECEEEHHHHHHH
EAERLGAVDYIGVGPIFPTISKADAEPESGTEILKEIRRAGIKLPIVGIGGINEANIAEV
HHHHCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
LAAGADGVSVISAITRADDYQLVIANLI
HHCCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA