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The map label for this gene is phoE [H]

Identifier: 116871658

GI number: 116871658

Start: 253703

End: 254338

Strand: Direct

Name: phoE [H]

Synonym: lwe0238

Alternate gene names: 116871658

Gene position: 253703-254338 (Clockwise)

Preceding gene: 116871657

Following gene: 116871659

Centisome position: 9.02

GC content: 39.62

Gene sequence:

>636_bases
TTGGCAGAAGGTTTACGTACGATTTATTTTGTAAGGCACGGTAAAACAGAATGGAATATGACTGGACAAATGCAAGGCTG
GGGAGATTCTCCTTTAGTTGCAGAAGGTATTGATGGCGCTAAAGCAGTCGGTGAAGTGTTGAAGGATACGAAGATTGACG
CAGTTTATACAAGTACAAGTAAGCGCACGAAAGATACAGCAGCTTATATTCTTGGCAACCGCGAAATCGAAGTTCAACCA
CTGGAAGAGCTAAAAGAGATGAGCTTTGGAACGTGGGAAGGTATTACTGTAACGGAAATTGACGAAAAACATCCCGAAGA
ACGAGCAAAAATTCTTCATAGTCCAGAAACCTACAAAGCAGAAGTTAATGGTGGCGAGACTTATTATGAGCTTGCAGAAC
GACTACTTCAAGGTGTCGAAAAAATCATCGCTGAAAATCCAAGTGGGAATATTCTTGTAGTTTCTCACGGAATGTCGCTT
ACTTTATTATTATACTTGCTGCAAGGTGGAACCATTGAAGATCATCGCAAAGAAGCCCCAAGAATTTTGAACACAAGTAT
TAGCATCGTAGAATACCAAAACGGCGAATTCTCTCTTAAACAATTAAATGAAACTGGTCATTTAGACTTAAAATAA

Upstream 100 bases:

>100_bases
ATTTAAAACCACTTGATACAGATAAATAAATAAGAAAACCTTGAAAATATGCCTATTCATGATACAATTTTAGAAGAATG
TGTAATTAGGAGGCAGAATT

Downstream 100 bases:

>100_bases
GTAATATCTTGGGCATATCTCATTTGGGGTATGTCCTAGATTTATAAGAGAGAGCTAGAAAAAGGGAGAAATAATACATA
TGAAAAGAATTGTCAAAATT

Product: phosphoglycerate mutase family protein

Products: 3-phospho-D-glycerate

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAEGLRTIYFVRHGKTEWNMTGQMQGWGDSPLVAEGIDGAKAVGEVLKDTKIDAVYTSTSKRTKDTAAYILGNREIEVQP
LEELKEMSFGTWEGITVTEIDEKHPEERAKILHSPETYKAEVNGGETYYELAERLLQGVEKIIAENPSGNILVVSHGMSL
TLLLYLLQGGTIEDHRKEAPRILNTSISIVEYQNGEFSLKQLNETGHLDLK

Sequences:

>Translated_211_residues
MAEGLRTIYFVRHGKTEWNMTGQMQGWGDSPLVAEGIDGAKAVGEVLKDTKIDAVYTSTSKRTKDTAAYILGNREIEVQP
LEELKEMSFGTWEGITVTEIDEKHPEERAKILHSPETYKAEVNGGETYYELAERLLQGVEKIIAENPSGNILVVSHGMSL
TLLLYLLQGGTIEDHRKEAPRILNTSISIVEYQNGEFSLKQLNETGHLDLK
>Mature_210_residues
AEGLRTIYFVRHGKTEWNMTGQMQGWGDSPLVAEGIDGAKAVGEVLKDTKIDAVYTSTSKRTKDTAAYILGNREIEVQPL
EELKEMSFGTWEGITVTEIDEKHPEERAKILHSPETYKAEVNGGETYYELAERLLQGVEKIIAENPSGNILVVSHGMSLT
LLLYLLQGGTIEDHRKEAPRILNTSISIVEYQNGEFSLKQLNETGHLDLK

Specific function: Phosphatase with broad substrate specificity. Does not have phosphoglycerate mutase activtiy [H]

COG id: COG0406

COG function: function code G; Fructose-2,6-bisphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate mutase family. GpmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI64762445, Length=172, Percent_Identity=37.7906976744186, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI64762406, Length=172, Percent_Identity=37.7906976744186, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI118582286, Length=172, Percent_Identity=34.3023255813954, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4758902, Length=166, Percent_Identity=33.7349397590361, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1786857, Length=174, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1790856, Length=186, Percent_Identity=30.1075268817204, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25145314, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17510917, Length=175, Percent_Identity=29.1428571428571, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993390, Length=175, Percent_Identity=29.1428571428571, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324857, Length=211, Percent_Identity=29.3838862559242, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643252, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137706, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643264, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643254, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643260, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643258, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643256, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643262, Length=167, Percent_Identity=35.9281437125749, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85725270, Length=133, Percent_Identity=34.5864661654135, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI85725272, Length=133, Percent_Identity=34.5864661654135, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24650981, Length=133, Percent_Identity=34.5864661654135, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013078
- InterPro:   IPR001345 [H]

Pfam domain/function: PF00300 PGAM [H]

EC number: 5.4.2.1

Molecular weight: Translated: 23564; Mature: 23433

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGLRTIYFVRHGKTEWNMTGQMQGWGDSPLVAEGIDGAKAVGEVLKDTKIDAVYTSTS
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEECCC
KRTKDTAAYILGNREIEVQPLEELKEMSFGTWEGITVTEIDEKHPEERAKILHSPETYKA
CCCCCCEEEEECCCEEEECCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEE
EVNGGETYYELAERLLQGVEKIIAENPSGNILVVSHGMSLTLLLYLLQGGTIEDHRKEAP
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHCC
RILNTSISIVEYQNGEFSLKQLNETGHLDLK
HHHCCCEEEEEECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
AEGLRTIYFVRHGKTEWNMTGQMQGWGDSPLVAEGIDGAKAVGEVLKDTKIDAVYTSTS
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEECCC
KRTKDTAAYILGNREIEVQPLEELKEMSFGTWEGITVTEIDEKHPEERAKILHSPETYKA
CCCCCCEEEEECCCEEEECCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEE
EVNGGETYYELAERLLQGVEKIIAENPSGNILVVSHGMSLTLLLYLLQGGTIEDHRKEAP
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHCC
RILNTSISIVEYQNGEFSLKQLNETGHLDLK
HHHCCCEEEEEECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-phospho-D-glycerate

Specific reaction: 2-phospho-D-glycerate = 3-phospho-D-glycerate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]