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The map label for this gene is ksgA [H]

Identifier: 116871577

GI number: 116871577

Start: 157741

End: 158628

Strand: Direct

Name: ksgA [H]

Synonym: lwe0157

Alternate gene names: 116871577

Gene position: 157741-158628 (Clockwise)

Preceding gene: 116871576

Following gene: 116871578

Centisome position: 5.61

GC content: 34.91

Gene sequence:

>888_bases
ATGAGTAAAGATATCGCAACTCCCGGAAAAACAACAGAAATCTTAAAAAAATATGGTTTCTTATTTAAAAAAAGTCTCGG
TCAAAACTTTTTGATTGATAGTAATATTTTGACACGAATCACTGATACAGCTGGAATTTCAAAAGAAACAAATGTAATTG
AAATTGGCCCTGGGATTGGCGCATTAACAGAACAATTAGCAAAAACAGCCAATGAAGTAGTTGCGTTTGAAATTGACCAA
CGTTTATTACCGATTTTGGATGACACGTTAAGTGCTTATAATAATATTCAAGTAGTTCATGGAGATGTTTTGAAAGCAGA
TGTAGAAGAAGTAATCACGCAACAATTCTCTAAATCAGAATTGCCGCTTAAAATCGTTGCGAACCTGCCATATTACGTAA
CCACGCCAATCATTTTAAAACTACTTCATGATAATATCCCAGCAGATTCCATGACTTTTATGCTTCAAAAAGAAGTGGCT
GACCGGATTAGCGCAGTTCCAAGCACGAAAAGTTATGGTAGTTTAACTATTGCTATTCAGTTCTATATGGAAGCCGAATT
AGCTTTTATCGTGCCTAAAACAGTTTTTATGCCGCAACCTAATGTTGATTCCGCAGTCATCCATTTAAAACGTCGAAAAG
AACCGCTTGCTAAAGTAAACGATGAAGAATTTTTCTTTGAAGTGACAAGAGCTTCTTTTGCTCAACGAAGAAAAACATTA
TGGAACAATTTAGCTTCGAAGTTCCCGGCTTTGAAACCGCGTAAAGATGAGTTAGTAGAAGGTTTAAATGCAATTGGTAT
AGACTTAATCCGTCGTGGAGAAACATTGGATATACCTGAATTCGCTAAATTAAGTAATTTTCTAGCTGATTTTTTAGAAG
AAAAATAA

Upstream 100 bases:

>100_bases
TACACAAATGGAAAACAATTACAAACAAGACTAGAGTCATTTGCCATTAGTGAAGAACAGTTAGTGGCTGCGTGCCAAAA
GATTATGCAGGAGGAAGAGA

Downstream 100 bases:

>100_bases
TTTTTTTCAAAAAGAGCGCTTTCTTAGTTGACAGTTAGCCCTTCTAACTGTTAAAATATTTGGTTATTGATTTCGAACCT
CATCTATGGTATAATAAGTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSKDIATPGKTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAGISKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQ
RLLPILDDTLSAYNNIQVVHGDVLKADVEEVITQQFSKSELPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVA
DRISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAKVNDEEFFFEVTRASFAQRRKTL
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLADFLEEK

Sequences:

>Translated_295_residues
MSKDIATPGKTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAGISKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQ
RLLPILDDTLSAYNNIQVVHGDVLKADVEEVITQQFSKSELPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVA
DRISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAKVNDEEFFFEVTRASFAQRRKTL
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLADFLEEK
>Mature_294_residues
SKDIATPGKTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAGISKETNVIEIGPGIGALTEQLAKTANEVVAFEIDQR
LLPILDDTLSAYNNIQVVHGDVLKADVEEVITQQFSKSELPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVAD
RISAVPSTKSYGSLTIAIQFYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAKVNDEEFFFEVTRASFAQRRKTLW
NNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLADFLEEK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=301, Percent_Identity=30.5647840531561, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=32.8888888888889, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786236, Length=278, Percent_Identity=32.3741007194245, Blast_Score=148, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=296, Percent_Identity=28.7162162162162, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=225, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=30.0448430493274, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=33.7662337662338, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357273, Length=252, Percent_Identity=27.3809523809524, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 33046; Mature: 32914

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIATPGKTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAGISKETNVIEIGPGIG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCCCCEEEECCCCH
ALTEQLAKTANEVVAFEIDQRLLPILDDTLSAYNNIQVVHGDVLKADVEEVITQQFSKSE
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCC
LPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVADRISAVPSTKSYGSLTIAIQ
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
FYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAKVNDEEFFFEVTRASFAQRRKTL
EEECCCEEEEECCEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLADFLEEK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDIATPGKTTEILKKYGFLFKKSLGQNFLIDSNILTRITDTAGISKETNVIEIGPGIG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCCCCEEEECCCCH
ALTEQLAKTANEVVAFEIDQRLLPILDDTLSAYNNIQVVHGDVLKADVEEVITQQFSKSE
HHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCC
LPLKIVANLPYYVTTPIILKLLHDNIPADSMTFMLQKEVADRISAVPSTKSYGSLTIAIQ
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
FYMEAELAFIVPKTVFMPQPNVDSAVIHLKRRKEPLAKVNDEEFFFEVTRASFAQRRKTL
EEECCCEEEEECCEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHH
WNNLASKFPALKPRKDELVEGLNAIGIDLIRRGETLDIPEFAKLSNFLADFLEEK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]