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The map label for this gene is metS [H]

Identifier: 116871573

GI number: 116871573

Start: 152598

End: 154595

Strand: Direct

Name: metS [H]

Synonym: lwe0153

Alternate gene names: 116871573

Gene position: 152598-154595 (Clockwise)

Preceding gene: 116871572

Following gene: 116871574

Centisome position: 5.42

GC content: 37.69

Gene sequence:

>1998_bases
GTGTTGCCTGAAGAGAAAAATACGTTTTATATTACAACACCAATCTATTATCCAAGCGGAAAAGCGCATATCGGACATGC
CTATACGACAGTTGCGGGGGACGCGATGGCTCGTTACAAACGCTTAAAAGGATATGATGTGTTTTACTTAACTGGAACAG
ATGAACATGGTCAGAAAATCCAAGCAAAAGCAAAAGAACGTGGAATTTCTGAACAAGAATACGTGGATGAAATTGCAGAA
GGTTTCCAAGAACTTTGGAAAAAACTAGAAATTTCTAATACAGATTTTATTCGTACAACACAAGACCGTCATAAAACATC
CGTAGAAAAAATCTTTGAACAACTTTTAGCACAAGGCGATATCTACTTAGGTGAATATGAAGGTTGGTACTCTGTTTCTG
ATGAAGAATATTTTACAGAAACTCAGTTAGAAGAAGTGTATAAGGACGAAAATGGCAAAGTAATTGGCGGGAAAGCTCCA
AGCGGCAATGAAGTCGAGCTTGTTAAAGAAGAATCTTACTTTTTCCGTATGAGCAAATACGCAGATCGTTTAGTAGAATA
TTATAATTCCCATCCAGAATTTATCCTACCAGAATCAAGAAAAAATGAAATGATTAATAATTTCATTAAACCAGGCTTAG
AGGATTTAGCAGTATCTAGAACAACTTTTGATTGGGGTATTAAAGTTCCTGGAAATCCTAAACACGTTGTATATGTGTGG
ATTGATGCACTTTCAAACTATATTACTGCGCTAGGATATAACACGGATAATGATACGAAATTCCAAAAATACTGGCCAGC
GGATGTACAGATTGTTGGGAAAGAAATTGTTCGTTTCCATACAATTTATTGGCCAATTATGTTAATGGCGCTTGACTTGC
CTCTTCCGAAAATGGTATTTGGCCATGGTTGGATTTTAATGAAAGATGGTAAAATGTCGAAATCTAAAGGGAATGTGGTA
GATCCTTATATGTTGATTGAGCGCTATGGCTTAGATGCGCTTCGTTACTACTTATTGCGCGAAGTTCCTTTTGGTTCAGA
TGGTTTATTTACGCCAGAAGACTTTGTTGACCGTGTAAACTATGACTTGGCTAATGATCTAGGTAATCTACTTAATCGAA
CAGTAGCAATGATTAACAAATACTTTGATGGAGAAATTCCAGCATATCAAGGAAATGTAACAGATTTCGATCAAACTTTA
GTAGATTTCAAAAATAATGTGGTAGAAGAATATGAGAAAAGCATGGAACATATGCAGTTTTCTGTAGCATTAAACCAGCT
TTGGTCGCTCATTTCTCGGACAAACAAATATATCGATGAAACAGCTCCATGGACACTTGCCAAGGAAGAAGATAAACGCA
CGGAACTAGCGAGCGTCATGACACATTTAGCAGAAAACTTGCGTATTATCGCGGTACTATTACAACCATTCCTAACAAGA
ACACCAGGCGAAATCTTCTTACAACTAGGACTTCAAGAAGAAAACTTAAAAAAATGGGATAGCATTTATGGTTATGGAGA
AATCCCAGCCGGTACAACAGTGGTGAAAAAAGGTACGCCAATTTTCCCAAGACTGGATGCGGAAGTTGAAGTTACGTATA
TTCAAGATGAAATGAAAGGTTCTGCCCCTGCTCCAGCTGAAGAAGTTGCGGAAGTCGTAGCACTTGAAACACCGCAAATC
GGCATTGAAGATTTCGACAAAATCGATCTTCGTGTTGCAGAAGTAAAACAAGTAGAAAAAGTGAAAAAAGCGGACAAACT
TCTTTGTTTCCAATTAGACCTAGGTGAAGGCAAACTACGCCAAGTGCTATCAGGTATTGCAGAATTCTATGAACCTGAAG
ACTTAATCGGCAAAAAAGTTATCGTCGTTTCTAACTTAAAACCTGTGAAACTTCGCGGATTAATGAGCGAAGGTATGATT
CTCTCAGGAGAAAAAGATGGCAAACTAAGCGTCATTGAAGCAAGTAATACACTTCCGAATGGCGCAAAAGTAAAATAA

Upstream 100 bases:

>100_bases
GAATTTTAATTGGTGTGGCAAAAGGTGCTTAATTAATTATTTCGTTTAGCGCGAAAAATGATATAATGATACTAATTGTT
TTATTTTTAAGGAGGGAATT

Downstream 100 bases:

>100_bases
TAAGGAAATGAGCCCTAATTCACTTGAATGTGGGCTCATTATATTTATATAGAAGTAATTAAATGAAGCTAAGAACAAAA
TCATTATATGTCTCGTCTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAE
GFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLAQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAP
SGNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVW
IDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVV
DPYMLIERYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNYDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTDFDQTL
VDFKNNVVEEYEKSMEHMQFSVALNQLWSLISRTNKYIDETAPWTLAKEEDKRTELASVMTHLAENLRIIAVLLQPFLTR
TPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLDAEVEVTYIQDEMKGSAPAPAEEVAEVVALETPQI
GIEDFDKIDLRVAEVKQVEKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYEPEDLIGKKVIVVSNLKPVKLRGLMSEGMI
LSGEKDGKLSVIEASNTLPNGAKVK

Sequences:

>Translated_665_residues
MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAE
GFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLAQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAP
SGNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVW
IDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVV
DPYMLIERYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNYDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTDFDQTL
VDFKNNVVEEYEKSMEHMQFSVALNQLWSLISRTNKYIDETAPWTLAKEEDKRTELASVMTHLAENLRIIAVLLQPFLTR
TPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLDAEVEVTYIQDEMKGSAPAPAEEVAEVVALETPQI
GIEDFDKIDLRVAEVKQVEKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYEPEDLIGKKVIVVSNLKPVKLRGLMSEGMI
LSGEKDGKLSVIEASNTLPNGAKVK
>Mature_665_residues
MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAE
GFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLAQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAP
SGNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVW
IDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVV
DPYMLIERYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNYDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTDFDQTL
VDFKNNVVEEYEKSMEHMQFSVALNQLWSLISRTNKYIDETAPWTLAKEEDKRTELASVMTHLAENLRIIAVLLQPFLTR
TPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLDAEVEVTYIQDEMKGSAPAPAEEVAEVVALETPQI
GIEDFDKIDLRVAEVKQVEKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYEPEDLIGKKVIVVSNLKPVKLRGLMSEGMI
LSGEKDGKLSVIEASNTLPNGAKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=34.0699815837937, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI14043022, Length=622, Percent_Identity=26.6881028938907, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI4507947, Length=81, Percent_Identity=40.7407407407407, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=28.8261515601783, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=35.5140186915888, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=510, Percent_Identity=33.7254901960784, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=32.8282828282828, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17540794, Length=595, Percent_Identity=26.0504201680672, Blast_Score=160, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321610, Length=518, Percent_Identity=33.5907335907336, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321704, Length=471, Percent_Identity=25.6900212314225, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21358483, Length=497, Percent_Identity=33.8028169014084, Blast_Score=293, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24655311, Length=582, Percent_Identity=26.9759450171821, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357223, Length=76, Percent_Identity=40.7894736842105, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24651924, Length=99, Percent_Identity=35.3535353535354, Blast_Score=67, Evalue=6e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75869; Mature: 75869

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKI
CCCCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
QAKAKERGISEQEYVDEIAEGFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLAQGD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCC
IYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSGNEVELVKEESYFFRMSKY
EEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHH
ADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVW
HHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHHHCCEEEECEEECCCCCCEEEEEE
IDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVF
HHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GHGWILMKDGKMSKSKGNVVDPYMLIERYGLDALRYYLLREVPFGSDGLFTPEDFVDRVN
CCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCC
YDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTDFDQTLVDFKNNVVEEYEKSMEHMQF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVALNQLWSLISRTNKYIDETAPWTLAKEEDKRTELASVMTHLAENLRIIAVLLQPFLTR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLDAEVEVTYIQDEMKG
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEEHHHCC
SAPAPAEEVAEVVALETPQIGIEDFDKIDLRVAEVKQVEKVKKADKLLCFQLDLGEGKLR
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
QVLSGIAEFYEPEDLIGKKVIVVSNLKPVKLRGLMSEGMILSGEKDGKLSVIEASNTLPN
HHHHHHHHHCCCHHHCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEECCCCCCC
GAKVK
CCCCC
>Mature Secondary Structure
MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKI
CCCCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
QAKAKERGISEQEYVDEIAEGFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLAQGD
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCC
IYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSGNEVELVKEESYFFRMSKY
EEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHH
ADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVW
HHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHHHCCEEEECEEECCCCCCEEEEEE
IDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVF
HHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GHGWILMKDGKMSKSKGNVVDPYMLIERYGLDALRYYLLREVPFGSDGLFTPEDFVDRVN
CCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCC
YDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTDFDQTLVDFKNNVVEEYEKSMEHMQF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVALNQLWSLISRTNKYIDETAPWTLAKEEDKRTELASVMTHLAENLRIIAVLLQPFLTR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLDAEVEVTYIQDEMKG
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEEHHHCC
SAPAPAEEVAEVVALETPQIGIEDFDKIDLRVAEVKQVEKVKKADKLLCFQLDLGEGKLR
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHH
QVLSGIAEFYEPEDLIGKKVIVVSNLKPVKLRGLMSEGMILSGEKDGKLSVIEASNTLPN
HHHHHHHHHCCCHHHCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCCEEEEECCCCCCC
GAKVK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA