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The map label for this gene is dosP [H]

Identifier: 116871522

GI number: 116871522

Start: 106287

End: 106985

Strand: Direct

Name: dosP [H]

Synonym: lwe0102

Alternate gene names: 116871522

Gene position: 106287-106985 (Clockwise)

Preceding gene: 116871521

Following gene: 116871524

Centisome position: 3.78

GC content: 33.05

Gene sequence:

>699_bases
GTGAAATTCCAACTTTTTATTCAACCAAAGTTAGATGTTCTTCAAGGTAATATTGTCGAATATGAAGTGCTTTTGCGCGA
TGATAGTGAAGTTCCTAGATTTCCTTTGTCGGAGCTAGAGGCTGTGCTTGCAGATGAAAACTTATACTATGTATTTACAG
AGTGGTTTTGTCATGCTTTTTTTGAGGTCTTAAATAAATATCCCAATGATCGATTTGCGATTAACATAGCACCCCAACAA
CTTTTTTATATGGAAACTGTTCATTTACTTGATAAATTACGAAGTGAAAGTCATCGAATAACTATAGAAATAACCGAAGA
CATTTTTGATGTTCCGGCCCATAAAAGACATTTGAATGCCAATGACAAAGATGCCTTTATTCTAAATAAAATTAAAGTAA
TCCATGGACTGGGTTATCATATTGCAATGGATGACGTGGGTTGTGGGCTGAATAGCTTAGAACGAGTAATGAGCTACCTT
CCATATATCACAGAAATTAAATTCTCTTTGCTTCATTTCAAAGATATTAATTTAGAAGATTTACTTTTGTTTATTAAAGC
ATGGGCGAATTTTTCTCAAAAAAACAATCTTGATTTTGTTGTTGAAGGTATTGAAACAAAAGAAACCATGGCGATATTAG
AAAATCACGGTGTTTCCATTTTTCAAGGTTACTTAGTTAATAAGCCATTCCCAGTATGA

Upstream 100 bases:

>100_bases
CATTTTTCGGAATGCCTGTTTTTTATTGGACTAAATTTTGCGGTTAGTAGTAAAAATGTATTAGAATAGAGAATAGAAAC
AGGAAGGGATGATAGGATTC

Downstream 100 bases:

>100_bases
AAGCTTTCTACTTGTGCCACTAATTTTTCATAAGAACTTATAATAATCGGTTTTTTTGAAAGAAGTAGTCCACTTGCACG
AATGGTTTCAAGTGCTTCTG

Product: EAL domain-containing protein

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKFQLFIQPKLDVLQGNIVEYEVLLRDDSEVPRFPLSELEAVLADENLYYVFTEWFCHAFFEVLNKYPNDRFAINIAPQQ
LFYMETVHLLDKLRSESHRITIEITEDIFDVPAHKRHLNANDKDAFILNKIKVIHGLGYHIAMDDVGCGLNSLERVMSYL
PYITEIKFSLLHFKDINLEDLLLFIKAWANFSQKNNLDFVVEGIETKETMAILENHGVSIFQGYLVNKPFPV

Sequences:

>Translated_232_residues
MKFQLFIQPKLDVLQGNIVEYEVLLRDDSEVPRFPLSELEAVLADENLYYVFTEWFCHAFFEVLNKYPNDRFAINIAPQQ
LFYMETVHLLDKLRSESHRITIEITEDIFDVPAHKRHLNANDKDAFILNKIKVIHGLGYHIAMDDVGCGLNSLERVMSYL
PYITEIKFSLLHFKDINLEDLLLFIKAWANFSQKNNLDFVVEGIETKETMAILENHGVSIFQGYLVNKPFPV
>Mature_232_residues
MKFQLFIQPKLDVLQGNIVEYEVLLRDDSEVPRFPLSELEAVLADENLYYVFTEWFCHAFFEVLNKYPNDRFAINIAPQQ
LFYMETVHLLDKLRSESHRITIEITEDIFDVPAHKRHLNANDKDAFILNKIKVIHGLGYHIAMDDVGCGLNSLERVMSYL
PYITEIKFSLLHFKDINLEDLLLFIKAWANFSQKNNLDFVVEGIETKETMAILENHGVSIFQGYLVNKPFPV

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=139, Percent_Identity=31.6546762589928, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082096, Length=200, Percent_Identity=26, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 27073; Mature: 27073

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQLFIQPKLDVLQGNIVEYEVLLRDDSEVPRFPLSELEAVLADENLYYVFTEWFCHAF
CEEEEEECCCHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHH
FEVLNKYPNDRFAINIAPQQLFYMETVHLLDKLRSESHRITIEITEDIFDVPAHKRHLNA
HHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCHHHCCCC
NDKDAFILNKIKVIHGLGYHIAMDDVGCGLNSLERVMSYLPYITEIKFSLLHFKDINLED
CCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHEEECCCCHHH
LLLFIKAWANFSQKNNLDFVVEGIETKETMAILENHGVSIFQGYLVNKPFPV
HHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEHEECCCCCCC
>Mature Secondary Structure
MKFQLFIQPKLDVLQGNIVEYEVLLRDDSEVPRFPLSELEAVLADENLYYVFTEWFCHAF
CEEEEEECCCHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHH
FEVLNKYPNDRFAINIAPQQLFYMETVHLLDKLRSESHRITIEITEDIFDVPAHKRHLNA
HHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCHHHCCCC
NDKDAFILNKIKVIHGLGYHIAMDDVGCGLNSLERVMSYLPYITEIKFSLLHFKDINLED
CCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHEEECCCCHHH
LLLFIKAWANFSQKNNLDFVVEGIETKETMAILENHGVSIFQGYLVNKPFPV
HHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]