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The map label for this gene is lip2 [H]

Identifier: 116871521

GI number: 116871521

Start: 105056

End: 106174

Strand: Direct

Name: lip2 [H]

Synonym: lwe0101

Alternate gene names: 116871521

Gene position: 105056-106174 (Clockwise)

Preceding gene: 116871520

Following gene: 116871522

Centisome position: 3.73

GC content: 38.34

Gene sequence:

>1119_bases
ATGAATTATAAACAAGCTTTAAACTATTTAAAATCAAATAGCAACATAGAACAAGAAGAAGGAACAGAAGTTATTTTCAA
AATGGCAGCGGATACATCTCGAGGGGATTTGGATCCATTTTTGTTAAAAGACCGTGAAAAAGAACTGGAAGGAACAAATG
CTACGATTGAAAAAATGCCACAAGAAATCAAAATGCCAGATTTTTCAAATTTAGAAGTGGCAACTGCAGCTGCTCTTCAA
ATGAGAGCAACTATGGGTAGCGATAATATCGATTTATCTAATGGAGTAACGACCGATCATAAAATAGTTCAAGGTAATTA
CGGCGATATCCCAGTTCGGATTTATCGTCAGGAAGAGACGACGAAATTAGTACCAGCATTGATTTTTTATCATGGTGGTG
GCTTTGTTGGTGGAACTCCAACTGTAGTAGAGAACTTTTGTAAAGGAATCGCAGAAAAATTACCTGCTGTCGTTATTAAT
GTGGACTATCACTTGGCGCCAGAATTTCCAGCCCCCGCAGCGCCAAAAGATTGTTTCCGAGTACTTGAGTGGGTAGCGGA
AAATAGTGCTGAGCTTGGTGTGGTTGCTTCAAAAATAGGTGTTTCTGGAGATAGTGCAGGCGGAACTTTAGCAGCAGCGG
TTAGTTATATGGATAGAGAGGCGAAGACCAATTACGTTGGATTCCAAGCATTATTATATCCAGCATTAACTTTGATAGAT
GAAGATAATGAAAAATACCAATGGGATATTTCCAAATTTAGCGCATCTGATGACACAATTCCACTTGTTGCACCAGGAAT
AATTGGAATGAATAGCTCCGTTGAATTATTACGAACTGTCTATGTGAGAGATGAAAATCCCGCTTCACCAATCTATTCTC
CATTATCAGCGGCAGATAAAAGTATTTATCCACCAACGTTGATAGCTAGTGCGGAGTTTGATGCCCTAAGAGCTTTTGCG
GATATTTTTGCAAAAGAGTTACGAACAAGTGGTGTAAAAACGAAAGCGATTGTCTACCAAGGGATGTGTCACGCTTTTAT
AGATAAATACGGCATTTTTCCTCAAGCAGAAGATGTAGCAGATGAAATAGTTCAAATGATGAAAGAAGTATTTAAATAA

Upstream 100 bases:

>100_bases
TAAACTTTTCAAAAAATATTATGGATTCCCACCATCAGAAAATTGGCGCAAATCGAGTTGAAGTATAAAATGAGTAAATT
TAGAAAGAAGGAAAATAATC

Downstream 100 bases:

>100_bases
CATTTTTGCAGGCATTTTTCGGAATGCCTGTTTTTTATTGGACTAAATTTTGCGGTTAGTAGTAAAAATGTATTAGAATA
GAGAATAGAAACAGGAAGGG

Product: lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNYKQALNYLKSNSNIEQEEGTEVIFKMAADTSRGDLDPFLLKDREKELEGTNATIEKMPQEIKMPDFSNLEVATAAALQ
MRATMGSDNIDLSNGVTTDHKIVQGNYGDIPVRIYRQEETTKLVPALIFYHGGGFVGGTPTVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCFRVLEWVAENSAELGVVASKIGVSGDSAGGTLAAAVSYMDREAKTNYVGFQALLYPALTLID
EDNEKYQWDISKFSASDDTIPLVAPGIIGMNSSVELLRTVYVRDENPASPIYSPLSAADKSIYPPTLIASAEFDALRAFA
DIFAKELRTSGVKTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEVFK

Sequences:

>Translated_372_residues
MNYKQALNYLKSNSNIEQEEGTEVIFKMAADTSRGDLDPFLLKDREKELEGTNATIEKMPQEIKMPDFSNLEVATAAALQ
MRATMGSDNIDLSNGVTTDHKIVQGNYGDIPVRIYRQEETTKLVPALIFYHGGGFVGGTPTVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCFRVLEWVAENSAELGVVASKIGVSGDSAGGTLAAAVSYMDREAKTNYVGFQALLYPALTLID
EDNEKYQWDISKFSASDDTIPLVAPGIIGMNSSVELLRTVYVRDENPASPIYSPLSAADKSIYPPTLIASAEFDALRAFA
DIFAKELRTSGVKTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEVFK
>Mature_372_residues
MNYKQALNYLKSNSNIEQEEGTEVIFKMAADTSRGDLDPFLLKDREKELEGTNATIEKMPQEIKMPDFSNLEVATAAALQ
MRATMGSDNIDLSNGVTTDHKIVQGNYGDIPVRIYRQEETTKLVPALIFYHGGGFVGGTPTVVENFCKGIAEKLPAVVIN
VDYHLAPEFPAPAAPKDCFRVLEWVAENSAELGVVASKIGVSGDSAGGTLAAAVSYMDREAKTNYVGFQALLYPALTLID
EDNEKYQWDISKFSASDDTIPLVAPGIIGMNSSVELLRTVYVRDENPASPIYSPLSAADKSIYPPTLIASAEFDALRAFA
DIFAKELRTSGVKTKAIVYQGMCHAFIDKYGIFPQAEDVADEIVQMMKEVFK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=297, Percent_Identity=26.5993265993266, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI206597554, Length=287, Percent_Identity=27.5261324041812, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI21328446, Length=100, Percent_Identity=38, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI68051721, Length=161, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157041239, Length=155, Percent_Identity=29.6774193548387, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786682, Length=280, Percent_Identity=24.6428571428571, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=278, Percent_Identity=25.8992805755396, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=151, Percent_Identity=30.4635761589404, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=153, Percent_Identity=30.718954248366, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 40712; Mature: 40712

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKQALNYLKSNSNIEQEEGTEVIFKMAADTSRGDLDPFLLKDREKELEGTNATIEKMP
CCHHHHHHHHHCCCCCCHHCCHHEEHHHHCCCCCCCCCHHHHCCCHHHCCCCCCHHHHCH
QEIKMPDFSNLEVATAAALQMRATMGSDNIDLSNGVTTDHKIVQGNYGDIPVRIYRQEET
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCH
TKLVPALIFYHGGGFVGGTPTVVENFCKGIAEKLPAVVINVDYHLAPEFPAPAAPKDCFR
HHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCHHHHH
VLEWVAENSAELGVVASKIGVSGDSAGGTLAAAVSYMDREAKTNYVGFQALLYPALTLID
HHHHHHCCCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
EDNEKYQWDISKFSASDDTIPLVAPGIIGMNSSVELLRTVYVRDENPASPIYSPLSAADK
CCCCCEEEEHHHCCCCCCCCEEEECCEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCC
SIYPPTLIASAEFDALRAFADIFAKELRTSGVKTKAIVYQGMCHAFIDKYGIFPQAEDVA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEIVQMMKEVFK
HHHHHHHHHHHC
>Mature Secondary Structure
MNYKQALNYLKSNSNIEQEEGTEVIFKMAADTSRGDLDPFLLKDREKELEGTNATIEKMP
CCHHHHHHHHHCCCCCCHHCCHHEEHHHHCCCCCCCCCHHHHCCCHHHCCCCCCHHHHCH
QEIKMPDFSNLEVATAAALQMRATMGSDNIDLSNGVTTDHKIVQGNYGDIPVRIYRQEET
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCH
TKLVPALIFYHGGGFVGGTPTVVENFCKGIAEKLPAVVINVDYHLAPEFPAPAAPKDCFR
HHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCHHHHH
VLEWVAENSAELGVVASKIGVSGDSAGGTLAAAVSYMDREAKTNYVGFQALLYPALTLID
HHHHHHCCCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
EDNEKYQWDISKFSASDDTIPLVAPGIIGMNSSVELLRTVYVRDENPASPIYSPLSAADK
CCCCCEEEEHHHCCCCCCCCEEEECCEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCC
SIYPPTLIASAEFDALRAFADIFAKELRTSGVKTKAIVYQGMCHAFIDKYGIFPQAEDVA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEIVQMMKEVFK
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]