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The map label for this gene is ilvB [H]

Identifier: 116696388

GI number: 116696388

Start: 2772137

End: 2773786

Strand: Direct

Name: ilvB [H]

Synonym: H16_B2452

Alternate gene names: 116696388

Gene position: 2772137-2773786 (Clockwise)

Preceding gene: 116696387

Following gene: 116696389

Centisome position: 95.18

GC content: 64.24

Gene sequence:

>1650_bases
GTGCCGGCCCGCTCAGGGGCCGCTGTTCTTGCCGACCTGATCCGAGAGTATGGGCACCGGCATGTCTTCCAGATCCCTGG
CGAGGGCATCCTCGAGCCACTGGATGCCCTCGCTACCCGCCATCCCGAAATCAGCCTCGTCACCTTCCGCCACGAGGCAG
GCATGACCTACGCCGCGCAGGCCTACGCGCGGCTTGGCGACCGGCCTTCCATCTGCATGGCGGGTCGCGCTCCGGGCGCC
CTGAACACAGCCCTGGCGCTGCATACCGCCGATACGGATAGTGCGGCGATGATTCTTGTCGTCGGGCAGCCGGCAGCCGC
AGAGGCTGAGCGCGACCCGATGTCAGGATTTGATTTGCAGCAGGCGTTACGCCCGCTCGTCAAGGACGTCATCACTGTCA
CCACCGCCACGAGACTGCAGGAAGGGATGATGCGCGCATGGTGCAAGGCCAGCAGCGGCCGGCCCGGGCCGGTGGTCCTG
GTTGTCACCGAAAATGTGTGGCGCGAGGATGCCACCTGCGCTCCGCTGCCTGCGCCCTCCATACCAGAGCCCGGTCCGGC
GCCAGGGCAAGCCGGTGCGGTTGCGGGCCTGCTCCGCGAAGCCCGTCGCCCACTAGTGCTGGTAGGCGGCAGTGGCTGGA
ATGACGCAGCGCGACTACGCCTGCATGCCTTGGCACAAGCCCACAACTGGCCAGTCGCCGCTGGCTATCGACGAGCAGAT
CTGTTCGACAACGACGACCCACACTATGCGGGCGAACTCGGCATCGGTCTCAATCCGCTGCTGGCAGAGCGGATAGTGCA
AGCCGACCTGATACTCGCCATCAACCTTCGCCTCGGCGAGATCAATACGTTCGGGACCGGTGGCTTCGGTGGATTCCGTC
TGCTCGATGCCCCGCGGCCGGCCCAGCGCCTGGTTCATGTGCATTCCAGCGCCGACGAACTCAACCGCATTTATCGCGCC
GACGTGGCGATCCAAGCTGCTCCAGCGCCCTTTCTTGAGGCGCTTTCAACAGCCCTTTCTGCGACTTCTTGGTCTTCCCC
TGATTCCGACTGGCCAGAGGGCTTTCGCATCGCGCGCGCGGCGTTCGTCGGGAAGGGTGACTGTCCAGGAGCTGTCGACC
TGCGAAAAGTCTTTATGACGCTGCGAGAGCGCCTCCCTGACAACACCGTCTTTACCGCTGGCGCGGGTGCCTACGCGATC
TGGCTACATCGCTATCTTGCATTCCGCCAGCCCGGCACCATGCTTGGCGCCAAGAGCGGTGCCATGGGATACGGATTGCC
GGCCGCCATTGGCGCAGCGATGGCGCGGCCGGATCGGCTGGTTGTGGCCGTGGCCGGCGATGGCTGCCTGCTCATGCATG
GTGAAGAACTGGCGACGGCAGTCCAGCAAAATCTGCGCATCATCATTATCGTTATCAATAACCAGGGATATGGCGCCATC
CGAAGTGCCCAGATCCGGCAGTTCGGGCGTGCTGTCGGCAGCGCCTTGCGAAACCCGGATTTCGTAGCCTATGCAAGAGC
CTTCGGCGCCCTCGCGGAACGCGTTCAGACGACCGCGGAATTCCTGCCGGCTCTGAAGCGAGTACTGAATGGAACCGGGC
CGGCGCTGATCGAGATTGCCGTACCCGACACCGTAGCGAAGCCCGAATGA

Upstream 100 bases:

>100_bases
CTAACCGGGCCCCAGGCATCGCGGCACGGCGGGGAGATGGCCCCCGCCTTGCCGCAGCCTCGCCCAACCTGTTGCACTAC
TTGTGATGAATCATGAATCC

Downstream 100 bases:

>100_bases
ACCAACCTATCTCCATCGTCGTCGTTGGTGCCGGTATTTCCGGACTCACCGCAGCTTACCGGCTCACGGCTGCCGGCTTC
ACCGTCCAGGTTCTGGAAAG

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPARSGAAVLADLIREYGHRHVFQIPGEGILEPLDALATRHPEISLVTFRHEAGMTYAAQAYARLGDRPSICMAGRAPGA
LNTALALHTADTDSAAMILVVGQPAAAEAERDPMSGFDLQQALRPLVKDVITVTTATRLQEGMMRAWCKASSGRPGPVVL
VVTENVWREDATCAPLPAPSIPEPGPAPGQAGAVAGLLREARRPLVLVGGSGWNDAARLRLHALAQAHNWPVAAGYRRAD
LFDNDDPHYAGELGIGLNPLLAERIVQADLILAINLRLGEINTFGTGGFGGFRLLDAPRPAQRLVHVHSSADELNRIYRA
DVAIQAAPAPFLEALSTALSATSWSSPDSDWPEGFRIARAAFVGKGDCPGAVDLRKVFMTLRERLPDNTVFTAGAGAYAI
WLHRYLAFRQPGTMLGAKSGAMGYGLPAAIGAAMARPDRLVVAVAGDGCLLMHGEELATAVQQNLRIIIIVINNQGYGAI
RSAQIRQFGRAVGSALRNPDFVAYARAFGALAERVQTTAEFLPALKRVLNGTGPALIEIAVPDTVAKPE

Sequences:

>Translated_549_residues
MPARSGAAVLADLIREYGHRHVFQIPGEGILEPLDALATRHPEISLVTFRHEAGMTYAAQAYARLGDRPSICMAGRAPGA
LNTALALHTADTDSAAMILVVGQPAAAEAERDPMSGFDLQQALRPLVKDVITVTTATRLQEGMMRAWCKASSGRPGPVVL
VVTENVWREDATCAPLPAPSIPEPGPAPGQAGAVAGLLREARRPLVLVGGSGWNDAARLRLHALAQAHNWPVAAGYRRAD
LFDNDDPHYAGELGIGLNPLLAERIVQADLILAINLRLGEINTFGTGGFGGFRLLDAPRPAQRLVHVHSSADELNRIYRA
DVAIQAAPAPFLEALSTALSATSWSSPDSDWPEGFRIARAAFVGKGDCPGAVDLRKVFMTLRERLPDNTVFTAGAGAYAI
WLHRYLAFRQPGTMLGAKSGAMGYGLPAAIGAAMARPDRLVVAVAGDGCLLMHGEELATAVQQNLRIIIIVINNQGYGAI
RSAQIRQFGRAVGSALRNPDFVAYARAFGALAERVQTTAEFLPALKRVLNGTGPALIEIAVPDTVAKPE
>Mature_548_residues
PARSGAAVLADLIREYGHRHVFQIPGEGILEPLDALATRHPEISLVTFRHEAGMTYAAQAYARLGDRPSICMAGRAPGAL
NTALALHTADTDSAAMILVVGQPAAAEAERDPMSGFDLQQALRPLVKDVITVTTATRLQEGMMRAWCKASSGRPGPVVLV
VTENVWREDATCAPLPAPSIPEPGPAPGQAGAVAGLLREARRPLVLVGGSGWNDAARLRLHALAQAHNWPVAAGYRRADL
FDNDDPHYAGELGIGLNPLLAERIVQADLILAINLRLGEINTFGTGGFGGFRLLDAPRPAQRLVHVHSSADELNRIYRAD
VAIQAAPAPFLEALSTALSATSWSSPDSDWPEGFRIARAAFVGKGDCPGAVDLRKVFMTLRERLPDNTVFTAGAGAYAIW
LHRYLAFRQPGTMLGAKSGAMGYGLPAAIGAAMARPDRLVVAVAGDGCLLMHGEELATAVQQNLRIIIIVINNQGYGAIR
SAQIRQFGRAVGSALRNPDFVAYARAFGALAERVQTTAEFLPALKRVLNGTGPALIEIAVPDTVAKPE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=24.7386759581882, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21361361, Length=569, Percent_Identity=23.9015817223199, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.5974499089253, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=26.508226691042, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=23.7383177570093, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=22.6937269372694, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788716, Length=527, Percent_Identity=23.1499051233397, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=23.6933797909408, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=23.6933797909408, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=25.6140350877193, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=21.0633946830266, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922626, Length=585, Percent_Identity=25.4700854700855, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58287; Mature: 58155

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARSGAAVLADLIREYGHRHVFQIPGEGILEPLDALATRHPEISLVTFRHEAGMTYAAQ
CCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
AYARLGDRPSICMAGRAPGALNTALALHTADTDSAAMILVVGQPAAAEAERDPMSGFDLQ
HHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHH
QALRPLVKDVITVTTATRLQEGMMRAWCKASSGRPGPVVLVVTENVWREDATCAPLPAPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
IPEPGPAPGQAGAVAGLLREARRPLVLVGGSGWNDAARLRLHALAQAHNWPVAAGYRRAD
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECC
LFDNDDPHYAGELGIGLNPLLAERIVQADLILAINLRLGEINTFGTGGFGGFRLLDAPRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECCEEECCCCCCCCEEEECCCCH
AQRLVHVHSSADELNRIYRADVAIQAAPAPFLEALSTALSATSWSSPDSDWPEGFRIARA
HHHHHHHHCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AFVGKGDCPGAVDLRKVFMTLRERLPDNTVFTAGAGAYAIWLHRYLAFRQPGTMLGAKSG
HEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCEECCCCC
AMGYGLPAAIGAAMARPDRLVVAVAGDGCLLMHGEELATAVQQNLRIIIIVINNQGYGAI
CCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCCH
RSAQIRQFGRAVGSALRNPDFVAYARAFGALAERVQTTAEFLPALKRVLNGTGPALIEIA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VPDTVAKPE
CCCCCCCCC
>Mature Secondary Structure 
PARSGAAVLADLIREYGHRHVFQIPGEGILEPLDALATRHPEISLVTFRHEAGMTYAAQ
CCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
AYARLGDRPSICMAGRAPGALNTALALHTADTDSAAMILVVGQPAAAEAERDPMSGFDLQ
HHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHH
QALRPLVKDVITVTTATRLQEGMMRAWCKASSGRPGPVVLVVTENVWREDATCAPLPAPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
IPEPGPAPGQAGAVAGLLREARRPLVLVGGSGWNDAARLRLHALAQAHNWPVAAGYRRAD
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECC
LFDNDDPHYAGELGIGLNPLLAERIVQADLILAINLRLGEINTFGTGGFGGFRLLDAPRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECCEEECCCCCCCCEEEECCCCH
AQRLVHVHSSADELNRIYRADVAIQAAPAPFLEALSTALSATSWSSPDSDWPEGFRIARA
HHHHHHHHCCHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AFVGKGDCPGAVDLRKVFMTLRERLPDNTVFTAGAGAYAIWLHRYLAFRQPGTMLGAKSG
HEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCEECCCCC
AMGYGLPAAIGAAMARPDRLVVAVAGDGCLLMHGEELATAVQQNLRIIIIVINNQGYGAI
CCCCCCHHHHHHHHCCCCEEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCCH
RSAQIRQFGRAVGSALRNPDFVAYARAFGALAERVQTTAEFLPALKRVLNGTGPALIEIA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VPDTVAKPE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]