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The map label for this gene is nirE [H]

Identifier: 116696221

GI number: 116696221

Start: 2584668

End: 2585441

Strand: Direct

Name: nirE [H]

Synonym: H16_B2285

Alternate gene names: 116696221

Gene position: 2584668-2585441 (Clockwise)

Preceding gene: 116696220

Following gene: 116696223

Centisome position: 88.74

GC content: 71.71

Gene sequence:

>774_bases
ATGAATCCACCCCTGAAACCCGGCAAGGTCTGGCTGGTCGGCGTCGGCCCGGGTAGCCCGGACCTGGTCACGGTACGCGC
CATGCGCGCGCTGGCCGCGGCCGAGGTCTGGCTGGTCGATGACCTGATCGCGCCGGAGATGTCCTGCTATGCCAGCCCCG
GCACCCATGTTGAGTGGGTCGGCAAGCGCGGCGGGCGCTGCTCGGTCAGCCAGGAACGCATCCAGAAGTTGACGCTGCAG
CACGCGCTGGCCGGCCGCAGCGTGGCGCGCGTCAAGGGTGGCGATCCGCTGCTGTTCGGGCGCGGCGGCGAAGAAGCCGC
CTTCCTGCGCGGCCACGGCGTGGCCGTCGAGATCGTCAATGGCATCAGCAGCGGCATCGCCGCCGCGCAGGCACTGGGGA
TTGCGCTGACGCACCGCGCGCACTGCCACGGCGTGACCTTCGTAACCGGCCATACCAGCGCGCACGGCTCGCCCGACTGG
CAGGCGCTGGTGCGTGGCGGCACCACGCTCGTGATCTATATGGGCATGAGCCGGCTCGCGGCGATCCGCGACGGCTTGCT
GGCGGCGGGCATGCGCAGCGACATGCCGGCGGCAGTGGTGATGCATGCCGGCAGCGAGCAGGCACGCAGCTGGACCGGCA
CGCTCGGCACGCTCGCCGAAGCGCTCGCGGCGGGGCTGGCCAGCCCGGCCGTGATGCTGGTGGGTGGGGTGGTTGCAGAC
ACGCTGCAAGCGGTGCACGCGATCGTGCCGGACGCTGGCTCGCTGGCCGCGTAG

Upstream 100 bases:

>100_bases
ACAAGCCCATCGGCAAGTACAACGTCTTCAACAAGACCACGCGCTCGGCCGGCACCAGCCACTGAGCCGGCGTGGTGATG
CCCGATCGCAGGAGCCCGAG

Downstream 100 bases:

>100_bases
CGTCGGCTGCCAGCAAGTTCTACCGAGTTCCGGCGACCCGATTGTGTGCTCCCTCTCCCGCTTGCGGGAGGGGGAGCAAA
CCCGCAGCATGCGGATGCCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNPPLKPGKVWLVGVGPGSPDLVTVRAMRALAAAEVWLVDDLIAPEMSCYASPGTHVEWVGKRGGRCSVSQERIQKLTLQ
HALAGRSVARVKGGDPLLFGRGGEEAAFLRGHGVAVEIVNGISSGIAAAQALGIALTHRAHCHGVTFVTGHTSAHGSPDW
QALVRGGTTLVIYMGMSRLAAIRDGLLAAGMRSDMPAAVVMHAGSEQARSWTGTLGTLAEALAAGLASPAVMLVGGVVAD
TLQAVHAIVPDAGSLAA

Sequences:

>Translated_257_residues
MNPPLKPGKVWLVGVGPGSPDLVTVRAMRALAAAEVWLVDDLIAPEMSCYASPGTHVEWVGKRGGRCSVSQERIQKLTLQ
HALAGRSVARVKGGDPLLFGRGGEEAAFLRGHGVAVEIVNGISSGIAAAQALGIALTHRAHCHGVTFVTGHTSAHGSPDW
QALVRGGTTLVIYMGMSRLAAIRDGLLAAGMRSDMPAAVVMHAGSEQARSWTGTLGTLAEALAAGLASPAVMLVGGVVAD
TLQAVHAIVPDAGSLAA
>Mature_257_residues
MNPPLKPGKVWLVGVGPGSPDLVTVRAMRALAAAEVWLVDDLIAPEMSCYASPGTHVEWVGKRGGRCSVSQERIQKLTLQ
HALAGRSVARVKGGDPLLFGRGGEEAAFLRGHGVAVEIVNGISSGIAAAQALGIALTHRAHCHGVTFVTGHTSAHGSPDW
QALVRGGTTLVIYMGMSRLAAIRDGLLAAGMRSDMPAAVVMHAGSEQARSWTGTLGTLAEALAAGLASPAVMLVGGVVAD
TLQAVHAIVPDAGSLAA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=37.6569037656904, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322922, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26250; Mature: 26250

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPLKPGKVWLVGVGPGSPDLVTVRAMRALAAAEVWLVDDLIAPEMSCYASPGTHVEWV
CCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEE
GKRGGRCSVSQERIQKLTLQHALAGRSVARVKGGDPLLFGRGGEEAAFLRGHGVAVEIVN
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEECCCEEEEEHH
GISSGIAAAQALGIALTHRAHCHGVTFVTGHTSAHGSPDWQALVRGGTTLVIYMGMSRLA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEECHHHHH
AIRDGLLAAGMRSDMPAAVVMHAGSEQARSWTGTLGTLAEALAAGLASPAVMLVGGVVAD
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
TLQAVHAIVPDAGSLAA
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNPPLKPGKVWLVGVGPGSPDLVTVRAMRALAAAEVWLVDDLIAPEMSCYASPGTHVEWV
CCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEE
GKRGGRCSVSQERIQKLTLQHALAGRSVARVKGGDPLLFGRGGEEAAFLRGHGVAVEIVN
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEECCCEEEEEHH
GISSGIAAAQALGIALTHRAHCHGVTFVTGHTSAHGSPDWQALVRGGTTLVIYMGMSRLA
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEECHHHHH
AIRDGLLAAGMRSDMPAAVVMHAGSEQARSWTGTLGTLAEALAAGLASPAVMLVGGVVAD
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
TLQAVHAIVPDAGSLAA
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]