Click here to switch to the map view.

The map label for this gene is mmsB [H]

Identifier: 116695686

GI number: 116695686

Start: 1988187

End: 1989086

Strand: Direct

Name: mmsB [H]

Synonym: H16_B1750

Alternate gene names: 116695686

Gene position: 1988187-1989086 (Clockwise)

Preceding gene: 116695685

Following gene: 116695687

Centisome position: 68.26

GC content: 64.0

Gene sequence:

>900_bases
ATGAGCAGAATTGCATTCATTGGTCTTGGCAATATGGGCATGCCCATGGCCCTGAACCTTGTACGTGCGGGCCACGACGT
GCAGGGATTCGATATCTCGGAAGCCGCACTGGCCGGCTTCGCCGGACAGGGGGGGCAATCGCAGCGCAGCGTGGCAGCGG
CTGTCGATGGCGCATCGATCGTCATTTCCATCGTGAGAAATGCCGATGACGTGAAGAACCTGTACTGCACCGCAGGCGGT
GTCCTGGACGTCGCCGCGCCGGGAGCCCTGCTGATCGAGAGCTCAACCATCGGACCCGCCGCCGCGCGTGCTGTCGCCGC
GGAAGCGGCGCAAGCCGGCTTTGCGATGTTGGATGCTCCCGTGGCCGGCGGCCAGGCTGGCGCCCGCGAAGCGCGGCTGA
CATTCATGGTCGGCGGCGCGCGCACGGCCTTCGACCGCGCTGAGCCAGTCCTGCGCCAGATGGGCGCCAGAATCTTTTAT
GCCGGGGCCAGCGGCAATGGCCAGATTGCCAAGCTGTGCAACAACCTGATCGCCTGCGTCAGCAGCGCGGTCGTCTCGGA
AGCCTTCATCCTGGGCAGCAAGCTCGGCATGGACTATCAGACGATGTACGACATCATCACACAGTCTACCGGCCAGTGCT
GGACCCTCTCGCACAACTGCCCGGTGCCGGGCCCCGTGCCGTCTTCGCCCGCCAGTCGCGATTATGTCCCTGGATTTGCG
GCGGATCTGATGCTCAAGGACCTCTCCCTCGTCGCGTCTGCCGCCAGTGAGGTTGGCGCCGCGACACCGTTTGGCGCCAG
GGCCATCCAGCTGTACCGGCAGCTAAGCGAATCTGGCCTTGGCGCCCGCGATTGGACCGTAGTTGCCAGGTTGATCGAAA
ACAGCGCTTCTCTCAATTAA

Upstream 100 bases:

>100_bases
GCTTCGCCCTCAACAGGACGATCGCGATGCGATCCGCCCCGGGCGTCGCACCGCACTGTGCGCAAGCGAACCGGCAACCG
ATAGCTGAGGGGTAAGCGAA

Downstream 100 bases:

>100_bases
TGCTGCATAGCCCAGGAGACCTCTGTGTCAGCTAACCCCCTCCATCCCAATCTCAACGCGGAAGATCTGGCCCTGGCCCT
GAGTGGGCACAATTTCATCG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSRIAFIGLGNMGMPMALNLVRAGHDVQGFDISEAALAGFAGQGGQSQRSVAAAVDGASIVISIVRNADDVKNLYCTAGG
VLDVAAPGALLIESSTIGPAAARAVAAEAAQAGFAMLDAPVAGGQAGAREARLTFMVGGARTAFDRAEPVLRQMGARIFY
AGASGNGQIAKLCNNLIACVSSAVVSEAFILGSKLGMDYQTMYDIITQSTGQCWTLSHNCPVPGPVPSSPASRDYVPGFA
ADLMLKDLSLVASAASEVGAATPFGARAIQLYRQLSESGLGARDWTVVARLIENSASLN

Sequences:

>Translated_299_residues
MSRIAFIGLGNMGMPMALNLVRAGHDVQGFDISEAALAGFAGQGGQSQRSVAAAVDGASIVISIVRNADDVKNLYCTAGG
VLDVAAPGALLIESSTIGPAAARAVAAEAAQAGFAMLDAPVAGGQAGAREARLTFMVGGARTAFDRAEPVLRQMGARIFY
AGASGNGQIAKLCNNLIACVSSAVVSEAFILGSKLGMDYQTMYDIITQSTGQCWTLSHNCPVPGPVPSSPASRDYVPGFA
ADLMLKDLSLVASAASEVGAATPFGARAIQLYRQLSESGLGARDWTVVARLIENSASLN
>Mature_298_residues
SRIAFIGLGNMGMPMALNLVRAGHDVQGFDISEAALAGFAGQGGQSQRSVAAAVDGASIVISIVRNADDVKNLYCTAGGV
LDVAAPGALLIESSTIGPAAARAVAAEAAQAGFAMLDAPVAGGQAGAREARLTFMVGGARTAFDRAEPVLRQMGARIFYA
GASGNGQIAKLCNNLIACVSSAVVSEAFILGSKLGMDYQTMYDIITQSTGQCWTLSHNCPVPGPVPSSPASRDYVPGFAA
DLMLKDLSLVASAASEVGAATPFGARAIQLYRQLSESGLGARDWTVVARLIENSASLN

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=36.271186440678, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=25.2595155709343, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI145693186, Length=297, Percent_Identity=30.6397306397306, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=30.5369127516779, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786719, Length=293, Percent_Identity=30.3754266211604, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789092, Length=293, Percent_Identity=32.0819112627986, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557316, Length=299, Percent_Identity=37.4581939799331, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=39.5904436860068, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=39.5904436860068, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=22.887323943662, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 30529; Mature: 30398

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIAFIGLGNMGMPMALNLVRAGHDVQGFDISEAALAGFAGQGGQSQRSVAAAVDGASI
CCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHH
VISIVRNADDVKNLYCTAGGVLDVAAPGALLIESSTIGPAAARAVAAEAAQAGFAMLDAP
HHHHHHCCHHHHHEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCHHEEECC
VAGGQAGAREARLTFMVGGARTAFDRAEPVLRQMGARIFYAGASGNGQIAKLCNNLIACV
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
SSAVVSEAFILGSKLGMDYQTMYDIITQSTGQCWTLSHNCPVPGPVPSSPASRDYVPGFA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHH
ADLMLKDLSLVASAASEVGAATPFGARAIQLYRQLSESGLGARDWTVVARLIENSASLN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRIAFIGLGNMGMPMALNLVRAGHDVQGFDISEAALAGFAGQGGQSQRSVAAAVDGASI
CEEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHH
VISIVRNADDVKNLYCTAGGVLDVAAPGALLIESSTIGPAAARAVAAEAAQAGFAMLDAP
HHHHHHCCHHHHHEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCCHHEEECC
VAGGQAGAREARLTFMVGGARTAFDRAEPVLRQMGARIFYAGASGNGQIAKLCNNLIACV
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
SSAVVSEAFILGSKLGMDYQTMYDIITQSTGQCWTLSHNCPVPGPVPSSPASRDYVPGFA
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHH
ADLMLKDLSLVASAASEVGAATPFGARAIQLYRQLSESGLGARDWTVVARLIENSASLN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]