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The map label for this gene is cysU [H]

Identifier: 116695540

GI number: 116695540

Start: 1804852

End: 1805751

Strand: Direct

Name: cysU [H]

Synonym: H16_B1601

Alternate gene names: 116695540

Gene position: 1804852-1805751 (Clockwise)

Preceding gene: 116695539

Following gene: 116695541

Centisome position: 61.97

GC content: 67.0

Gene sequence:

>900_bases
ATGGCTGTCTTCCCGCAAGCGGGGAGACAGCCGGGCGGCTCACCCAAGCCATCGCCCACCGCCCATCACCCCGTCACTGC
CATGACAAACCGATCCCCCTCCCTCTTCCTGTTTGCCTTGCCGGCACTGGTGGTCTTCCTGGCCTTCTTCTGCCTGCCGA
TGGCGCGGCTCGTGGCGGTCAGCATCAACGGCGAGCAAGGCGCGGGCGTGTACTGGACCGTGGTCTCCAGTCCGCGCTAC
CTGCACAGCCTGATGGTCACCGTGCTGCTGTCGGCAGCCGTGACACTGGTCACGCTGGTGATTGCCGGCATTGCCGGCAC
CTTCCTGCAGCGCAACGCGGTGCCTGGCAAGTCCGTGCTGGTGGCCATGCTGACCTTTCCGCTGGCGTTTCCCGGCGTGG
TGATCGGCTTCATGGTCATCATGCTCGGCGGCCGCAACGGCCTGCTGGCCATGGTCGGCGACAAGCTCGCCGGCGAGCGC
TGGACCTTTGCCTACGGCCTGGCCGGGCTGTTCGTCGGCTACCTGTACTTTTCGATCCCGCGCGTGATCCTGACCGTGAT
GGCGGCAGTGGAGAAGCTCGACGCCTCGCTGGAAGAAGCTGCCCGCTCGCTGGGCGCCGGGCCCTGGCGCGTGGTGCGCG
ACGTGATGCTGCCCGCGATGATGCCGGCCATGATCTCCAGCGGCGCGATCTGCTTTGCCACCAGCATGGGCGCGTTCGGT
ACCGCCTTCACGCTGGCCACGCAACTCGACGTGCTGCCGCTCACCATCTACAACGAGTTCACCAACTACGCCAACTTCGG
CATGGCCGCTGCGCTGTCCATCCTGTTGGGCACCGTCACCTGGGCCTTGCTGGCGCTCGCGCGCTGGTTTTCCGGCGACG
CCGTGGCCGCGACCGCCTAG

Upstream 100 bases:

>100_bases
TCGGCAAGATGGCCGACGTGCAAAAGGCGTTCAGCGAGCAGTATCTGAAGGAGGTGCGCTGACAAGCGCGCTGACGACTG
GCGATGGTGTCTCCCGCCTG

Downstream 100 bases:

>100_bases
GAGCCATCCCATGACCCCAACCCATCGCCGCCCCGGCTACTGGCTGCAACTGGCGCTCACACTGGCGGTCTGCGCCTTCA
TGACCGTGCCGGTCGTGCTC

Product: ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAVFPQAGRQPGGSPKPSPTAHHPVTAMTNRSPSLFLFALPALVVFLAFFCLPMARLVAVSINGEQGAGVYWTVVSSPRY
LHSLMVTVLLSAAVTLVTLVIAGIAGTFLQRNAVPGKSVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLAMVGDKLAGER
WTFAYGLAGLFVGYLYFSIPRVILTVMAAVEKLDASLEEAARSLGAGPWRVVRDVMLPAMMPAMISSGAICFATSMGAFG
TAFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGTVTWALLALARWFSGDAVAATA

Sequences:

>Translated_299_residues
MAVFPQAGRQPGGSPKPSPTAHHPVTAMTNRSPSLFLFALPALVVFLAFFCLPMARLVAVSINGEQGAGVYWTVVSSPRY
LHSLMVTVLLSAAVTLVTLVIAGIAGTFLQRNAVPGKSVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLAMVGDKLAGER
WTFAYGLAGLFVGYLYFSIPRVILTVMAAVEKLDASLEEAARSLGAGPWRVVRDVMLPAMMPAMISSGAICFATSMGAFG
TAFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGTVTWALLALARWFSGDAVAATA
>Mature_298_residues
AVFPQAGRQPGGSPKPSPTAHHPVTAMTNRSPSLFLFALPALVVFLAFFCLPMARLVAVSINGEQGAGVYWTVVSSPRYL
HSLMVTVLLSAAVTLVTLVIAGIAGTFLQRNAVPGKSVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLAMVGDKLAGERW
TFAYGLAGLFVGYLYFSIPRVILTVMAAVEKLDASLEEAARSLGAGPWRVVRDVMLPAMMPAMISSGAICFATSMGAFGT
AFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGTVTWALLALARWFSGDAVAATA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=197, Percent_Identity=30.4568527918782, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786980, Length=179, Percent_Identity=30.7262569832402, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786254, Length=208, Percent_Identity=25.9615384615385, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082099, Length=221, Percent_Identity=25.7918552036199, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787570, Length=232, Percent_Identity=25.8620689655172, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31458; Mature: 31327

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFPQAGRQPGGSPKPSPTAHHPVTAMTNRSPSLFLFALPALVVFLAFFCLPMARLVAV
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
SINGEQGAGVYWTVVSSPRYLHSLMVTVLLSAAVTLVTLVIAGIAGTFLQRNAVPGKSVL
EECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VAMLTFPLAFPGVVIGFMVIMLGGRNGLLAMVGDKLAGERWTFAYGLAGLFVGYLYFSIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
RVILTVMAAVEKLDASLEEAARSLGAGPWRVVRDVMLPAMMPAMISSGAICFATSMGAFG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TAFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGTVTWALLALARWFSGDAVAATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure 
AVFPQAGRQPGGSPKPSPTAHHPVTAMTNRSPSLFLFALPALVVFLAFFCLPMARLVAV
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
SINGEQGAGVYWTVVSSPRYLHSLMVTVLLSAAVTLVTLVIAGIAGTFLQRNAVPGKSVL
EECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VAMLTFPLAFPGVVIGFMVIMLGGRNGLLAMVGDKLAGERWTFAYGLAGLFVGYLYFSIP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHH
RVILTVMAAVEKLDASLEEAARSLGAGPWRVVRDVMLPAMMPAMISSGAICFATSMGAFG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TAFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGTVTWALLALARWFSGDAVAATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]