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The map label for this gene is metE [H]

Identifier: 116695522

GI number: 116695522

Start: 1782066

End: 1784444

Strand: Direct

Name: metE [H]

Synonym: H16_B1581

Alternate gene names: 116695522

Gene position: 1782066-1784444 (Clockwise)

Preceding gene: 116695520

Following gene: 116695529

Centisome position: 61.19

GC content: 69.86

Gene sequence:

>2379_bases
ATGGCACGCACCCATATCCTCGGCTTTCCCCGCATCGGCGAACGCCGCGAACTGAAATTTGCGCAAGAAGCGTTCTGGCG
CGGCGATCGCACCGAAGCCGAGCTGCGCGACGTGGCCGCGCAACTGCGCCGCCGCCACTGGCAGCTGCAGGCCGACCGCG
GCCTGGACACCGTGGCCACCGGCGACTTTGCCTATTACGACCAGATGCTGAGCCTGACCGCGCTGCTGGGTGCGCTGCCG
CGCCGCTTCGGCTTGGATCCGGCCCGGCTCACGCTGACGCAGTACTTCGAGCTGGCACGCGGCAACCGCGAGCAGCCGGC
CATGGAAATGACCAAGTGGTTCGACACCAACTACCACTACCTGGTCCCGGAGCTGGACGCCGAGACCACTTTCGACGGCG
GCCCGGCCTGGTTCTTCGAGGAAGCCGATGAGGCGCTGGCGCTTGGCCTGCGCGCCCGCCCGGTGCTGATCGGGCCGGTC
ACCTACCTGTGGCTGTCCAAGAGCCATGTGGCGGGCTTCGACCGCCTGGCCCTGCTGCCGCAACTGCTGCAGGGCTATCG
CCGCATCCTCGACCAGCTCAAGGCGCGCGGCATCGAATGGGTGCAGATCGACGAGCCGGCCTTGTGCCTGGACCTGGAGC
CGGCCTGGCTCGACGCATTCGACACCGCCTACGCTGCCTTGCGCGAAGCCGGCCCCAAGTTGCTGCTGGCCACCTATTTC
GACACCGCCGCCGACCATGCGCAGCGCGCCGCCGCCCTGCCGGTCGACGGCTTCCATATCGACCTGGTGCGGGCGCCGGA
ACAACTGGCCGCATGGCAGGCCGCGCTGCCCGCGCACGCCGTGCTGTCGCTGGGCGTGATCGACGGCCGCAATATCTGGC
GCACCGACCTGCGCCGAGTCCTGGACACGCTGCGCCCGGTGCAGGCCGCGCTGGGCGAGCGCCTGTGGCTGGCACCGTCG
TGCTCGCTGCTGCATGTGCCGGTGTCGCTCGCGCATGAAGTGCGTCTCGATGTCGAGCTGAAGTCGTGGCTCGCCTTTGC
CACCGAAAAGCTCGACGAGCTGTCCGTGCTGGGCCGCGCGTTGAATCAAGGCGACGCCGTGGTCGCCGAAGCGCTGGCAG
CATCCGACGCCGCGCAGGCCTCGCGCCGCGCCTCGCGCCGGGTGGTCAACCCGCGGGTGCAGCAGCGGCTGGCAGCCGTG
AGCGCCGGCATGGCCGACCGCGCCAGCCCCTTTGCCGAGCGCATCGAGCGCCAGCGCCAGGCGCTGCAGCTGCCGCTGTT
GCCGACCACCACGATCGGCTCGTTCCCTCAGACCGCGGCCATCCGCCAGACCCGCGCCGCGTTCAAGCGTGGCGAGATCG
GCGCGCTCGAATACCTGGAGCGCATCCGTGCCGAGATCGCGGTGGCCGTGCGCAAGCAGGAAGCACTGGGCCTGGACGTG
CTGGTGCACGGCGAAGCCGAGCGCAACGACATGGTCGAGTACTTCGGCGAGCAGCTGTGCGGCTACGGCTTTACCGAGAA
CGGCTGGGTGCAGAGCTATGGCTCGCGCTGCGTCAAGCCGCCGGTGATCTACGGCGACGTGTACCGGCCCGAGCCGATGA
CGGTCGATACCGCCCGCTACGCGCAATCGCTGACCGAGCGGCCGATGAAGGGCATGCTCACCGGCCCCATCACCATGCTG
CAGTGGTCCTTCGTGCGCGACGACCAGCCGCGTGCCACCACCGCGCGCCAGCTGGCCCTGGCCATCCGCGACGAAGTGTG
CGACCTGGAGCAGGCCGGCATCCGCGTGATCCAGATCGACGAGCCCGCGCTGCGCGAAGGCCTGCCGCTGCGCCGTGCCG
ACTGGGATGCCTACCTGGACTGGGCCGTCACTGCCTTCCGCCTGTCGGCCAGCGGCGTGCAGGACCAGACCCAGATCCAC
ACGCATATGTGCTATGCCGAGTTCAACGATATCCTGCCCGCCATCGCGGCCATGGACGCGGACGTGATCACCATCGAGAC
CTCGCGCTCGGCGATGGAACTGCTGGAAGGCTTCGGCGACTTCGACTACCCGAACGAGATCGGGCCGGGTGTCTACGACA
TCCACTCGCCGCGCGTGCCGTCGGTGCAGGCCATGGAGCGGCTGCTCGACCGCGCCTGCGAGGTGGTGCCGCCGCAGCGG
CTGTGGGTCAACCCGGACTGCGGCCTGAAGACGCGCGGCTGGGAAGAGACCGAAGCAGCGCTGGCCAATATGGTCAGCGC
GGCCCGCGCGCTGCGCGCGCGGCTGTCGGCGCGGGGCGCAACGACCTGGAAGCGCCTCAGCAAGCCTGCAGCCGCCACCG
CCGCGGCAGTGCCCCACGCCGGCAACGCCTGCACGGCCTGCGCCACGCACGCGAACTGA

Upstream 100 bases:

>100_bases
TTGATATGAAACTCATTCATATGTTGTTTAAAACTTGTCGCTTGATTCATTTTACGGGCAAACCGACACTGTGCGCCAGA
CATCTTCCGGAGCAACCTCC

Downstream 100 bases:

>100_bases
GACGGCGTAGCGCCAATTGCGCTGGCAGGAAAAGACAAAGGCCGCATGCCCCTCACAGGCATGCGGCCTTTGTCCTCGAT
GGCGGCCTCGTCAGTCCACC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVATGDFAYYDQMLSLTALLGALP
RRFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHYLVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPV
TYLWLSKSHVAGFDRLALLPQLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFDTAYAALREAGPKLLLATYF
DTAADHAQRAAALPVDGFHIDLVRAPEQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRVLDTLRPVQAALGERLWLAPS
CSLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRALNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQRLAAV
SAGMADRASPFAERIERQRQALQLPLLPTTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDV
LVHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITML
QWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIH
THMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQR
LWVNPDCGLKTRGWEETEAALANMVSAARALRARLSARGATTWKRLSKPAAATAAAVPHAGNACTACATHAN

Sequences:

>Translated_792_residues
MARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVATGDFAYYDQMLSLTALLGALP
RRFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHYLVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPV
TYLWLSKSHVAGFDRLALLPQLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFDTAYAALREAGPKLLLATYF
DTAADHAQRAAALPVDGFHIDLVRAPEQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRVLDTLRPVQAALGERLWLAPS
CSLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRALNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQRLAAV
SAGMADRASPFAERIERQRQALQLPLLPTTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDV
LVHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITML
QWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIH
THMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQR
LWVNPDCGLKTRGWEETEAALANMVSAARALRARLSARGATTWKRLSKPAAATAAAVPHAGNACTACATHAN
>Mature_791_residues
ARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVATGDFAYYDQMLSLTALLGALPR
RFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHYLVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPVT
YLWLSKSHVAGFDRLALLPQLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFDTAYAALREAGPKLLLATYFD
TAADHAQRAAALPVDGFHIDLVRAPEQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRVLDTLRPVQAALGERLWLAPSC
SLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRALNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQRLAAVS
AGMADRASPFAERIERQRQALQLPLLPTTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDVL
VHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQ
WSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIHT
HMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQRL
WVNPDCGLKTRGWEETEAALANMVSAARALRARLSARGATTWKRLSKPAAATAAAVPHAGNACTACATHAN

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=754, Percent_Identity=57.0291777188329, Blast_Score=847, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=773, Percent_Identity=46.7011642949547, Blast_Score=685, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 88040; Mature: 87909

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVAT
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCEEC
GDFAYYDQMLSLTALLGALPRRFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEE
LVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPVTYLWLSKSHVAGFDRLALLP
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCHHHCCHHHHHHHH
QLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFDTAYAALREAGPKLLLATYF
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEHH
DTAADHAQRAAALPVDGFHIDLVRAPEQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRV
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHH
LDTLRPVQAALGERLWLAPSCSLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRA
HHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHH
LNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQRLAAVSAGMADRASPFAERIERQRQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ALQLPLLPTTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDV
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LVHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARY
EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHH
AQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQID
HHHHHHCCHHCCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCEEEEEEC
EPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIHTHMCYAEFNDILPAIAAMDA
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQR
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCC
LWVNPDCGLKTRGWEETEAALANMVSAARALRARLSARGATTWKRLSKPAAATAAAVPHA
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCC
GNACTACATHAN
CCCHHHHCCCCC
>Mature Secondary Structure 
ARTHILGFPRIGERRELKFAQEAFWRGDRTEAELRDVAAQLRRRHWQLQADRGLDTVAT
CCCEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCEEC
GDFAYYDQMLSLTALLGALPRRFGLDPARLTLTQYFELARGNREQPAMEMTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEE
LVPELDAETTFDGGPAWFFEEADEALALGLRARPVLIGPVTYLWLSKSHVAGFDRLALLP
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCHHHCCHHHHHHHH
QLLQGYRRILDQLKARGIEWVQIDEPALCLDLEPAWLDAFDTAYAALREAGPKLLLATYF
HHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEHH
DTAADHAQRAAALPVDGFHIDLVRAPEQLAAWQAALPAHAVLSLGVIDGRNIWRTDLRRV
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHH
LDTLRPVQAALGERLWLAPSCSLLHVPVSLAHEVRLDVELKSWLAFATEKLDELSVLGRA
HHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHH
LNQGDAVVAEALAASDAAQASRRASRRVVNPRVQQRLAAVSAGMADRASPFAERIERQRQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ALQLPLLPTTTIGSFPQTAAIRQTRAAFKRGEIGALEYLERIRAEIAVAVRKQEALGLDV
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LVHGEAERNDMVEYFGEQLCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARY
EEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHH
AQSLTERPMKGMLTGPITMLQWSFVRDDQPRATTARQLALAIRDEVCDLEQAGIRVIQID
HHHHHHCCHHCCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHCHHHCCEEEEEEC
EPALREGLPLRRADWDAYLDWAVTAFRLSASGVQDQTQIHTHMCYAEFNDILPAIAAMDA
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVQAMERLLDRACEVVPPQR
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCC
LWVNPDCGLKTRGWEETEAALANMVSAARALRARLSARGATTWKRLSKPAAATAAAVPHA
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCC
GNACTACATHAN
CCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA