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The map label for this gene is mhpA [H]

Identifier: 116695487

GI number: 116695487

Start: 1735991

End: 1737733

Strand: Direct

Name: mhpA [H]

Synonym: H16_B1546

Alternate gene names: 116695487

Gene position: 1735991-1737733 (Clockwise)

Preceding gene: 116695486

Following gene: 116695488

Centisome position: 59.61

GC content: 68.96

Gene sequence:

>1743_bases
ATGGATGGAGACACAGGCATGAGCAGCATCGATTACCAGCGGCTGTCGTTCGAGTACCAGCCCTGCGCCGAGCAACAGAA
AGGGGCCGATCCGGCCGTCCACCCGGTCGTCGTGGTCGGCGCCGGGCCGGTCGGCCTGGCCACCGCCATCGACCTGGCGC
AGCGCGGCGTGGCCGTGGTGCTGGTGGATGACGACTGCACGCTGTCCACCGGATCGCGAGCAATCTGTTTTTCCAAGCGC
ACGCTGGATATCTTCGACCGGCTTGGCTGCGGCGAGCGCATGGTCGACAAGGGCGTGCGCTGGCACGTCGGCAAGGTGTT
CCTGCGCGACCAGCAGGTCTACAGCTTCGACCTGCTGCCCGAGAGCGGCCACCGCCGCCCTGCCTTCATCAACCTGCAGC
AGTACTACCTCGAAGGCTACCTGCTGGAGCGCGCGCAGGCGCTGCCCAATATCGAGATCCGCTGGCGCAACAGGGTGGTC
GGGCTGGAACAGCGCGGCACGTCCGAGGACGGCGTGATACTGACCATCGAGACGCCTGACGGCAGCTACCCGCTGGCGGC
ACGCTACGTGGTGGCCGCCGACGGTTCGCGCAGCCCGATGCGCAAGCTGCTGGGCCTGGACAGCAAGGGCCGCACCTTCC
ACGACCGCTTCCTGATTGCCGACGTGAAGATGGAAGCGGATTTCCCGAGCGAGCGCTGGTTCTGGTTCGACCCGCCCTTC
CACCCGAACCAGTCGGTGCTGCTGCACCGCCAGCCGGACAACGTCTGGCGCATCGACTTCCAGCTCGGCTGGGACGCCGA
CCCGGAGCTGGAGAAAACGCCGGAGCGCGTGATCCCGCGGGTGCAGGCGCTGCTGGGCAAGGACGTGAAGTTCGAGCTGG
AATGGGTCAGCGTCTATACGTTCTCCTGCCTTCGCATGGACAGTTTCCGCCACGGCAATATCCTGTTCGCCGGCGATGCC
GCGCATGGCGTGTCGCCGTTCGGCGCGCGTGGCGCCAACAGCGGCATCCAGGACGCCGAGAACCTGGCCTGGAAGCTGGC
CTATGTGCTGCAGGGCCATGCCTGCGACAAGCTGCTGGACACCTACGCCAGCGAGCGCGAATACGCCGCCGACGAGAACC
TGCGCAACTCCACACGTTCCACCGACTTCATCACGCCCAAGAGCGCGGTCAGCCGCGTGTTCCGCGATGCGGTGCTGACC
CTGTCCAGGCGCCATGCGTTTGCGCGCACCCTGGTCAACAGCGGGCGGCTGTCGGTGCCCGCGGTGCTGCAGGATTCGCC
GCTGAACACCGCTGACGATGCCCAGGACTATGCCTGCAAGCTGGTGCCTGGCGCGGTCTGCTGCGACGCGCCGGTATCCG
GCGCGCAGGGCGCGGGCTGGCTGCTGTCGCACCTGGGCGGGGACTTCACGGTGCTGGTGTTCGGCAGCCCTGACACCATC
GATGCAGCCACGCTCGCCGGCCTCGCCGCGCTGAAAGACAGCGGCGTGCCGCTGCGGCTGGTCTACGTGACCGACGCCGC
GCAGCCGGCGGTGACCTGCACCAATGCCACCGTGCTGCGCGACGACGAGGGCCTGGCCGCCGCGCGCTACGGTGCCACGC
CCGGCACCTGCTACCTGGTCCGCCCGGACCAGCATGTCTGCGCCCGCTGGCACCACCCCGACCCGGCCGCGATCCGCGCC
GCGCTGGCGCGTGCCACCGGTGCCGACCTGCGCGCCCCGCAGCCCGGCCGCATGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCGTCCGCCACCCGCGCATCTGGACGGCCCAGCGCGACAAGGAAATGTGGGCCGCACTGCAGGACTGACCTCCAAAAAG
AATAAGTCGGGCAACAAGCT

Downstream 100 bases:

>100_bases
GACCGGCCAGACCTGCACCGCGGAGACTCCCATGCCCAATACGCTCAACACCCAACCCAACCTGGCGCGGCCCGACGACT
TCTACGAAGCGCTGATCGAG

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MDGDTGMSSIDYQRLSFEYQPCAEQQKGADPAVHPVVVVGAGPVGLATAIDLAQRGVAVVLVDDDCTLSTGSRAICFSKR
TLDIFDRLGCGERMVDKGVRWHVGKVFLRDQQVYSFDLLPESGHRRPAFINLQQYYLEGYLLERAQALPNIEIRWRNRVV
GLEQRGTSEDGVILTIETPDGSYPLAARYVVAADGSRSPMRKLLGLDSKGRTFHDRFLIADVKMEADFPSERWFWFDPPF
HPNQSVLLHRQPDNVWRIDFQLGWDADPELEKTPERVIPRVQALLGKDVKFELEWVSVYTFSCLRMDSFRHGNILFAGDA
AHGVSPFGARGANSGIQDAENLAWKLAYVLQGHACDKLLDTYASEREYAADENLRNSTRSTDFITPKSAVSRVFRDAVLT
LSRRHAFARTLVNSGRLSVPAVLQDSPLNTADDAQDYACKLVPGAVCCDAPVSGAQGAGWLLSHLGGDFTVLVFGSPDTI
DAATLAGLAALKDSGVPLRLVYVTDAAQPAVTCTNATVLRDDEGLAAARYGATPGTCYLVRPDQHVCARWHHPDPAAIRA
ALARATGADLRAPQPGRMAA

Sequences:

>Translated_580_residues
MDGDTGMSSIDYQRLSFEYQPCAEQQKGADPAVHPVVVVGAGPVGLATAIDLAQRGVAVVLVDDDCTLSTGSRAICFSKR
TLDIFDRLGCGERMVDKGVRWHVGKVFLRDQQVYSFDLLPESGHRRPAFINLQQYYLEGYLLERAQALPNIEIRWRNRVV
GLEQRGTSEDGVILTIETPDGSYPLAARYVVAADGSRSPMRKLLGLDSKGRTFHDRFLIADVKMEADFPSERWFWFDPPF
HPNQSVLLHRQPDNVWRIDFQLGWDADPELEKTPERVIPRVQALLGKDVKFELEWVSVYTFSCLRMDSFRHGNILFAGDA
AHGVSPFGARGANSGIQDAENLAWKLAYVLQGHACDKLLDTYASEREYAADENLRNSTRSTDFITPKSAVSRVFRDAVLT
LSRRHAFARTLVNSGRLSVPAVLQDSPLNTADDAQDYACKLVPGAVCCDAPVSGAQGAGWLLSHLGGDFTVLVFGSPDTI
DAATLAGLAALKDSGVPLRLVYVTDAAQPAVTCTNATVLRDDEGLAAARYGATPGTCYLVRPDQHVCARWHHPDPAAIRA
ALARATGADLRAPQPGRMAA
>Mature_580_residues
MDGDTGMSSIDYQRLSFEYQPCAEQQKGADPAVHPVVVVGAGPVGLATAIDLAQRGVAVVLVDDDCTLSTGSRAICFSKR
TLDIFDRLGCGERMVDKGVRWHVGKVFLRDQQVYSFDLLPESGHRRPAFINLQQYYLEGYLLERAQALPNIEIRWRNRVV
GLEQRGTSEDGVILTIETPDGSYPLAARYVVAADGSRSPMRKLLGLDSKGRTFHDRFLIADVKMEADFPSERWFWFDPPF
HPNQSVLLHRQPDNVWRIDFQLGWDADPELEKTPERVIPRVQALLGKDVKFELEWVSVYTFSCLRMDSFRHGNILFAGDA
AHGVSPFGARGANSGIQDAENLAWKLAYVLQGHACDKLLDTYASEREYAADENLRNSTRSTDFITPKSAVSRVFRDAVLT
LSRRHAFARTLVNSGRLSVPAVLQDSPLNTADDAQDYACKLVPGAVCCDAPVSGAQGAGWLLSHLGGDFTVLVFGSPDTI
DAATLAGLAALKDSGVPLRLVYVTDAAQPAVTCTNATVLRDDEGLAAARYGATPGTCYLVRPDQHVCARWHHPDPAAIRA
ALARATGADLRAPQPGRMAA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=384, Percent_Identity=29.4270833333333, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 63525; Mature: 63525

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGDTGMSSIDYQRLSFEYQPCAEQQKGADPAVHPVVVVGAGPVGLATAIDLAQRGVAVV
CCCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCEEEE
LVDDDCTLSTGSRAICFSKRTLDIFDRLGCGERMVDKGVRWHVGKVFLRDQQVYSFDLLP
EECCCCEECCCCEEEEEECCHHHHHHHCCCCHHHHHCCCEEEEEEEEEECCEEEEEEECC
ESGHRRPAFINLQQYYLEGYLLERAQALPNIEIRWRNRVVGLEQRGTSEDGVILTIETPD
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCEEEEEECCC
GSYPLAARYVVAADGSRSPMRKLLGLDSKGRTFHDRFLIADVKMEADFPSERWFWFDPPF
CCCCEEEEEEEEECCCHHHHHHHHCCCCCCCEEECEEEEEEEEEECCCCCCCEEEECCCC
HPNQSVLLHRQPDNVWRIDFQLGWDADPELEKTPERVIPRVQALLGKDVKFELEWVSVYT
CCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEE
FSCLRMDSFRHGNILFAGDAAHGVSPFGARGANSGIQDAENLAWKLAYVLQGHACDKLLD
EEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
TYASEREYAADENLRNSTRSTDFITPKSAVSRVFRDAVLTLSRRHAFARTLVNSGRLSVP
HHHCCCCHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVLQDSPLNTADDAQDYACKLVPGAVCCDAPVSGAQGAGWLLSHLGGDFTVLVFGSPDTI
EEECCCCCCCCCCCHHCEEEECCCCEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCC
DAATLAGLAALKDSGVPLRLVYVTDAAQPAVTCTNATVLRDDEGLAAARYGATPGTCYLV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCEEEEECCCCCEEEECCCCCCEEEEE
RPDQHVCARWHHPDPAAIRAALARATGADLRAPQPGRMAA
CCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDGDTGMSSIDYQRLSFEYQPCAEQQKGADPAVHPVVVVGAGPVGLATAIDLAQRGVAVV
CCCCCCCCCCCHHHHEECCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCEEEE
LVDDDCTLSTGSRAICFSKRTLDIFDRLGCGERMVDKGVRWHVGKVFLRDQQVYSFDLLP
EECCCCEECCCCEEEEEECCHHHHHHHCCCCHHHHHCCCEEEEEEEEEECCEEEEEEECC
ESGHRRPAFINLQQYYLEGYLLERAQALPNIEIRWRNRVVGLEQRGTSEDGVILTIETPD
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCEEEEEECCC
GSYPLAARYVVAADGSRSPMRKLLGLDSKGRTFHDRFLIADVKMEADFPSERWFWFDPPF
CCCCEEEEEEEEECCCHHHHHHHHCCCCCCCEEECEEEEEEEEEECCCCCCCEEEECCCC
HPNQSVLLHRQPDNVWRIDFQLGWDADPELEKTPERVIPRVQALLGKDVKFELEWVSVYT
CCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEEEEEEE
FSCLRMDSFRHGNILFAGDAAHGVSPFGARGANSGIQDAENLAWKLAYVLQGHACDKLLD
EEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
TYASEREYAADENLRNSTRSTDFITPKSAVSRVFRDAVLTLSRRHAFARTLVNSGRLSVP
HHHCCCCHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVLQDSPLNTADDAQDYACKLVPGAVCCDAPVSGAQGAGWLLSHLGGDFTVLVFGSPDTI
EEECCCCCCCCCCCHHCEEEECCCCEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCC
DAATLAGLAALKDSGVPLRLVYVTDAAQPAVTCTNATVLRDDEGLAAARYGATPGTCYLV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCEEEEECCCCCEEEECCCCCCEEEEE
RPDQHVCARWHHPDPAAIRAALARATGADLRAPQPGRMAA
CCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA