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The map label for this gene is ampR [H]

Identifier: 116695466

GI number: 116695466

Start: 1713731

End: 1714618

Strand: Direct

Name: ampR [H]

Synonym: H16_B1525

Alternate gene names: 116695466

Gene position: 1713731-1714618 (Clockwise)

Preceding gene: 116695463

Following gene: 116695468

Centisome position: 58.84

GC content: 71.06

Gene sequence:

>888_bases
ATGATCCGCAGGCTCCCCTCGATGCAAGTCCTCCAGGCCTTTGCCGCCACCGCGCGCACGGGCAACATGACGCGCGCCGG
CGATGCGCTGTGCGTGACGCACGGCGCCATCAGCCGCCATATCCGTGCGCTGGAGGCACAGCTGGGCTGCACCCTGTTCC
TGCGCGCCGGCCGGCAACTGGAACTGACCGCCACCGGGCGCGAGCTTGCCGATGAACTGGAAAATGCCTTGCGCGCGGTC
GAACTGTCGCTGGCCGGTGCCACCGAGCGCCGCGCGCGTGAACGCCAGTCGCTGAGCGTCAATGTCAGCCCCGAGCTGGC
CGGCGCCTGGCTGGTACCCAAGCTGCCGCGGTTGCGCGAAGCCCTGCCCGGCCTGGACCTGTCTATCGCAGCCAGCACCG
ACGACCCGAACTTCGCCCGCTCCGACCTCGACGTCAGCCTGCGCTACGGTCCCGCGCCCTTCACCGGCTACCAGTGCGTG
AAGCTGCTGGACGACGACATCATCGCGGTATGCAGCCCCGACTTCGCGCAGCGGCACGCGGGCATGACGGCCGCAGACGC
CGCGCACCTGCCGCGGCTGCGGCACGCGCGCTTTCGCTGGGCGCAATGGGGCGCGGCGGCGGGGGTGGTGCTGGACGAGC
CGGAAGACGGCATCGTCTTCGACTGCCGCGCACTGCTGATCGAAGCCGCCGCCAGCGGCCTGGGGCTGGCGCTGACGCGC
GCCTTGCTGGCACGGGAGGCTATCGCCGCGGGCAGGCTGGTGCAGCCGTTGGCCGCCAGCTTTCCCGCGAGCCACAACTG
CTACATCGTCTGGCGTGGGGACAATCCCAAGCAGGCGCTGATCCGGCGCTTCGCGCTGTGGCTGCGCGACGCTGCGGTGC
AGGCGTGA

Upstream 100 bases:

>100_bases
GGCTTCGCAGTGGCCGGTTCGGTGCGGCCCAGTATCGGCCGCGCGGCATGGCAGGACAATCGACTTGTTTTGCCATGACC
TGTGAGGCAAGATCACAGCC

Downstream 100 bases:

>100_bases
CCGGCACCGCCCAGCCGCTAGCCCTTCCCTCCCCGCCACACCTGCAGCAGATCCCACGCCGGCGCTTCCCCTTCCGGCTG
TGGCAACGCTTGCGCCTTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MIRRLPSMQVLQAFAATARTGNMTRAGDALCVTHGAISRHIRALEAQLGCTLFLRAGRQLELTATGRELADELENALRAV
ELSLAGATERRARERQSLSVNVSPELAGAWLVPKLPRLREALPGLDLSIAASTDDPNFARSDLDVSLRYGPAPFTGYQCV
KLLDDDIIAVCSPDFAQRHAGMTAADAAHLPRLRHARFRWAQWGAAAGVVLDEPEDGIVFDCRALLIEAAASGLGLALTR
ALLAREAIAAGRLVQPLAASFPASHNCYIVWRGDNPKQALIRRFALWLRDAAVQA

Sequences:

>Translated_295_residues
MIRRLPSMQVLQAFAATARTGNMTRAGDALCVTHGAISRHIRALEAQLGCTLFLRAGRQLELTATGRELADELENALRAV
ELSLAGATERRARERQSLSVNVSPELAGAWLVPKLPRLREALPGLDLSIAASTDDPNFARSDLDVSLRYGPAPFTGYQCV
KLLDDDIIAVCSPDFAQRHAGMTAADAAHLPRLRHARFRWAQWGAAAGVVLDEPEDGIVFDCRALLIEAAASGLGLALTR
ALLAREAIAAGRLVQPLAASFPASHNCYIVWRGDNPKQALIRRFALWLRDAAVQA
>Mature_295_residues
MIRRLPSMQVLQAFAATARTGNMTRAGDALCVTHGAISRHIRALEAQLGCTLFLRAGRQLELTATGRELADELENALRAV
ELSLAGATERRARERQSLSVNVSPELAGAWLVPKLPRLREALPGLDLSIAASTDDPNFARSDLDVSLRYGPAPFTGYQCV
KLLDDDIIAVCSPDFAQRHAGMTAADAAHLPRLRHARFRWAQWGAAAGVVLDEPEDGIVFDCRALLIEAAASGLGLALTR
ALLAREAIAAGRLVQPLAASFPASHNCYIVWRGDNPKQALIRRFALWLRDAAVQA

Specific function: This protein is a positive regulator of gene expression of cephalosporinase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=30.9859154929577, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=30.976430976431, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.3737024221453, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=24.2957746478873, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693193, Length=266, Percent_Identity=23.3082706766917, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=28.2485875706215, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31900; Mature: 31900

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRLPSMQVLQAFAATARTGNMTRAGDALCVTHGAISRHIRALEAQLGCTLFLRAGRQL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHEEECCCCEE
ELTATGRELADELENALRAVELSLAGATERRARERQSLSVNVSPELAGAWLVPKLPRLRE
EEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCHHHHH
ALPGLDLSIAASTDDPNFARSDLDVSLRYGPAPFTGYQCVKLLDDDIIAVCSPDFAQRHA
HCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCHHHHHHC
GMTAADAAHLPRLRHARFRWAQWGAAAGVVLDEPEDGIVFDCRALLIEAAASGLGLALTR
CCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHCCHHHHHHH
ALLAREAIAAGRLVQPLAASFPASHNCYIVWRGDNPKQALIRRFALWLRDAAVQA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRRLPSMQVLQAFAATARTGNMTRAGDALCVTHGAISRHIRALEAQLGCTLFLRAGRQL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHEEECCCCEE
ELTATGRELADELENALRAVELSLAGATERRARERQSLSVNVSPELAGAWLVPKLPRLRE
EEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCHHHHH
ALPGLDLSIAASTDDPNFARSDLDVSLRYGPAPFTGYQCVKLLDDDIIAVCSPDFAQRHA
HCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCHHHHHHC
GMTAADAAHLPRLRHARFRWAQWGAAAGVVLDEPEDGIVFDCRALLIEAAASGLGLALTR
CCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHCCHHHHHHH
ALLAREAIAAGRLVQPLAASFPASHNCYIVWRGDNPKQALIRRFALWLRDAAVQA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2788410 [H]