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The map label for this gene is alsR [H]

Identifier: 116695388

GI number: 116695388

Start: 1619362

End: 1620249

Strand: Direct

Name: alsR [H]

Synonym: H16_B1446

Alternate gene names: 116695388

Gene position: 1619362-1620249 (Clockwise)

Preceding gene: 116695376

Following gene: 116695392

Centisome position: 55.6

GC content: 65.54

Gene sequence:

>888_bases
ATGGAACTCCGGCAACTGCTCTATTTCGTGACCGTCGCGGATGAGTTGAACTTCAGCAAGGCGGCGGTGCGGCTGCACAT
GTCGCAGCCACCGCTCTCCCAGCAAATCAAGGCACTGGAAGACGAGATGGGCGTGGAGCTGTTGGTGCGCAATCGCCGGG
AAGTCCGCCTGACCGACGCGGGCCGTGCCTTCCAGGCAGACTGCCGCGCCGTGCTCGAACAGGTGCGCGTAGCCGTGACC
ACCGCGCGGCGCACGGCGGCCGGCGACATCGGCACACTGCAGATCGGCATGGTGACGTCAGGCATCTTCCATGTGCTGCC
TACCATCCTGACGCGCATGGCAGAGCGTTTTCCCGGACTGCATATCGCCGTGACCGACATGGGCTCGGCCGACCAGGCGC
ATGCCGTGATGCAGGGCAAGCTCGATATCGGCATCGTGCATTCGATCCCGACGCGCAGCGGCCTCGGCAAGACGCCGATC
TTCACCGAGTATTTCACTATCGTCGTGCCCGAGGGCCACGAACTCGCCGCAAGGCCGGCGCTGGCGCTCCGCGACCTCGA
GGGCGTGCCGCTGGTGGCATTCTCGCGTGAGCACGCGCCGGCGCTGTTCGACGGCATGATCGCCTCATGCCTGCATGCCG
GCTTCAGTCCCGTCATTGCGCATACGGCGCGCAATCCGCTGACCACTTTCCAGATGGTCAAGCTCGGCCTGGGCGTGGCG
CTGGTGCCGCGTTCCTACGCCCGCGCCGGGCTGCCAGGCGTGGTGTTCCGCGAGATCGGCCAGACCGCTGGCCACATCCA
GCTCTATGCAATCTGGCGTGAGCAGGCGCCCAGCGATCTGGTCATGCGGGTCGTGAGCGAAGTGATGAGCGAACCGATCG
GCTACTAA

Upstream 100 bases:

>100_bases
TCGCACCATGAGTTATAGGGCTGGCATATGACACGGCAGCGCGCTACCCCGGGTTGGTTGCGGCTATCCTAGTACCTTGT
CCCGCAAGGAAATCAGCACC

Downstream 100 bases:

>100_bases
GCCGCATCTCGGCACCGTCATGCCGCCTTCAAGCCACCGTGCTTCGCGCCGGAGACAGCGCAGCCACATAGTCGATGATC
GCCGCCACAACCTCGTCCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELRQLLYFVTVADELNFSKAAVRLHMSQPPLSQQIKALEDEMGVELLVRNRREVRLTDAGRAFQADCRAVLEQVRVAVT
TARRTAAGDIGTLQIGMVTSGIFHVLPTILTRMAERFPGLHIAVTDMGSADQAHAVMQGKLDIGIVHSIPTRSGLGKTPI
FTEYFTIVVPEGHELAARPALALRDLEGVPLVAFSREHAPALFDGMIASCLHAGFSPVIAHTARNPLTTFQMVKLGLGVA
LVPRSYARAGLPGVVFREIGQTAGHIQLYAIWREQAPSDLVMRVVSEVMSEPIGY

Sequences:

>Translated_295_residues
MELRQLLYFVTVADELNFSKAAVRLHMSQPPLSQQIKALEDEMGVELLVRNRREVRLTDAGRAFQADCRAVLEQVRVAVT
TARRTAAGDIGTLQIGMVTSGIFHVLPTILTRMAERFPGLHIAVTDMGSADQAHAVMQGKLDIGIVHSIPTRSGLGKTPI
FTEYFTIVVPEGHELAARPALALRDLEGVPLVAFSREHAPALFDGMIASCLHAGFSPVIAHTARNPLTTFQMVKLGLGVA
LVPRSYARAGLPGVVFREIGQTAGHIQLYAIWREQAPSDLVMRVVSEVMSEPIGY
>Mature_295_residues
MELRQLLYFVTVADELNFSKAAVRLHMSQPPLSQQIKALEDEMGVELLVRNRREVRLTDAGRAFQADCRAVLEQVRVAVT
TARRTAAGDIGTLQIGMVTSGIFHVLPTILTRMAERFPGLHIAVTDMGSADQAHAVMQGKLDIGIVHSIPTRSGLGKTPI
FTEYFTIVVPEGHELAARPALALRDLEGVPLVAFSREHAPALFDGMIASCLHAGFSPVIAHTARNPLTTFQMVKLGLGVA
LVPRSYARAGLPGVVFREIGQTAGHIQLYAIWREQAPSDLVMRVVSEVMSEPIGY

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=33.6805555555556, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788887, Length=279, Percent_Identity=35.84229390681, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI2367136, Length=267, Percent_Identity=30.3370786516854, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=31.6872427983539, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=27.8688524590164, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=24.2798353909465, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=29.2, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788748, Length=241, Percent_Identity=28.2157676348548, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787806, Length=284, Percent_Identity=25.7042253521127, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787601, Length=189, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=265, Percent_Identity=26.0377358490566, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788297, Length=170, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=26.2948207171315, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789639, Length=120, Percent_Identity=34.1666666666667, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786401, Length=164, Percent_Identity=27.4390243902439, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=163, Percent_Identity=26.9938650306748, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=208, Percent_Identity=26.4423076923077, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=302, Percent_Identity=23.841059602649, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32138; Mature: 32138

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLLYFVTVADELNFSKAAVRLHMSQPPLSQQIKALEDEMGVELLVRNRREVRLTDA
CCHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHCHHHEECCCCEEEEECC
GRAFQADCRAVLEQVRVAVTTARRTAAGDIGTLQIGMVTSGIFHVLPTILTRMAERFPGL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
HIAVTDMGSADQAHAVMQGKLDIGIVHSIPTRSGLGKTPIFTEYFTIVVPEGHELAARPA
EEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCHHCCCH
LALRDLEGVPLVAFSREHAPALFDGMIASCLHAGFSPVIAHTARNPLTTFQMVKLGLGVA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCEE
LVPRSYARAGLPGVVFREIGQTAGHIQLYAIWREQAPSDLVMRVVSEVMSEPIGY
ECCCHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MELRQLLYFVTVADELNFSKAAVRLHMSQPPLSQQIKALEDEMGVELLVRNRREVRLTDA
CCHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHCHHHEECCCCEEEEECC
GRAFQADCRAVLEQVRVAVTTARRTAAGDIGTLQIGMVTSGIFHVLPTILTRMAERFPGL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
HIAVTDMGSADQAHAVMQGKLDIGIVHSIPTRSGLGKTPIFTEYFTIVVPEGHELAARPA
EEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCHHCCCH
LALRDLEGVPLVAFSREHAPALFDGMIASCLHAGFSPVIAHTARNPLTTFQMVKLGLGVA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCEE
LVPRSYARAGLPGVVFREIGQTAGHIQLYAIWREQAPSDLVMRVVSEVMSEPIGY
ECCCHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]