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The map label for this gene is phaA [H]

Identifier: 116695312

GI number: 116695312

Start: 1531113

End: 1532321

Strand: Direct

Name: phaA [H]

Synonym: H16_B1369

Alternate gene names: 116695312

Gene position: 1531113-1532321 (Clockwise)

Preceding gene: 116695311

Following gene: 116695313

Centisome position: 52.57

GC content: 69.64

Gene sequence:

>1209_bases
ATGCGCAGAGCTGCAATCGTCACTCCCCTCCGCACGCCCGTCGGCACCTTCGGCGGCAGCTTGCGCCCGGTGCCTGTGGA
GGAACTGGCCGCCACCGCCGTGCGCGCCGTGGTGGAACGCAGCGGCATCGATCCCGCGCGTATCGACGACGTGGTCTTTG
CCCAGTCCTACGCCAACAGCGAAGTGCCCTGCGTCGGCCGCTGGGCCGCGCTGCAGGCCGGCCTGCCGGTCGAGGTGCCG
GGCATGCAGCTGGACCGCCGCTGCGGCGGCGGCCTGCAGGCCATCGTCACGGCCTCGATGATGGTGCAAAGCGGCGCCGC
CGACGTGGTGATCGCGGGCGGCGTCGAGAGCATGAGCAATATCGAGTACTACACCACCGACATGCGCTGGGGCGCGCGCT
CGGGCAATGTGCGCTTCTTCGACCGCCTCGACCGTGGCCGTGAACGCTCCCAGCCGGTCGAGCGCTTCGGCAAGATCTCC
GGGATGATCGAGACCGCCGAGAACCTGGCGCGCGACTACGGCATCAGCCGCGAGGCGGCCGATGTCTTCGCCGCCCGCAG
CCACGCACGCGCCGCGGCAGCCTGGGAGGCCGGCCGCTTCGATGCCGAGGTCGTGCCCGTGCAGGTGCCCCAGCGCAAGG
GCGATCCGGTGCGGTTCGCGCGCGACGAAGGCTTCCGCCCGGAAACCACGCGTGAAAGCCTTGGCAAGCTGCGCACGCTG
ATGCCGAACGGTACCGTCACCGCTGGCAACGCCAGCCAGCAGAATGACGCGTCGGCCGCCTGCCTGATCGTGGCCGAAGA
CAAGCTGGCCGAACTGTGCCTGACCCCCATGGCCTCGCTGGTGGGCTGGGCGGCGGCCGGCTGCGAGCCCTCGCACATGG
GCATCGGCCCGGTGCCCGCGGTGAAGAAGCTGCTGGCGCGCCTGAACCTGACGCTGGACCAGATGGACCTGGTCGAGCTG
AACGAAGCCTTCGCCTGCCAGGTGCTGGCCGTGCTCAAGGGCTGGGAATGGCATGACCAGGATGCGATCGAGCAGAAGCT
GAACGTGAACGGCTCGGGCATCTCGCTGGGCCATCCGATCGGCGCCACCGGCGTGCGCATCCTGGCCACGCTGCTGCATG
AACTGCAGCGCCGTGGCGGCCGCTATGGCCTGGAAACCATGTGCATCGGCGGCGGCCAGGGTATTGCCGCGGTCTTCGAA
CGCTACTGA

Upstream 100 bases:

>100_bases
TGCTGCGTCAGTGCGGCGCGGCCTTCGTGGCCCTTCCCGGGCACACGCTGGCCAACTGACTTACTTGCTCATCCCAACAC
AGAGTTCCAAGGAGACTTCC

Downstream 100 bases:

>100_bases
GAAAACCCACTCCATTCACCGTACAGCCCCCGTGCGCGACGGCGGGGGCTGAGCCTGCCTTTTGCCTTTTGCTTTTCGGC
TTTTGCCATCGGCTCTTTGC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRRAAIVTPLRTPVGTFGGSLRPVPVEELAATAVRAVVERSGIDPARIDDVVFAQSYANSEVPCVGRWAALQAGLPVEVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYTTDMRWGARSGNVRFFDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGISREAADVFAARSHARAAAAWEAGRFDAEVVPVQVPQRKGDPVRFARDEGFRPETTRESLGKLRTL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELCLTPMASLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLTLDQMDLVEL
NEAFACQVLAVLKGWEWHDQDAIEQKLNVNGSGISLGHPIGATGVRILATLLHELQRRGGRYGLETMCIGGGQGIAAVFE
RY

Sequences:

>Translated_402_residues
MRRAAIVTPLRTPVGTFGGSLRPVPVEELAATAVRAVVERSGIDPARIDDVVFAQSYANSEVPCVGRWAALQAGLPVEVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYTTDMRWGARSGNVRFFDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGISREAADVFAARSHARAAAAWEAGRFDAEVVPVQVPQRKGDPVRFARDEGFRPETTRESLGKLRTL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELCLTPMASLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLTLDQMDLVEL
NEAFACQVLAVLKGWEWHDQDAIEQKLNVNGSGISLGHPIGATGVRILATLLHELQRRGGRYGLETMCIGGGQGIAAVFE
RY
>Mature_402_residues
MRRAAIVTPLRTPVGTFGGSLRPVPVEELAATAVRAVVERSGIDPARIDDVVFAQSYANSEVPCVGRWAALQAGLPVEVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYTTDMRWGARSGNVRFFDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGISREAADVFAARSHARAAAAWEAGRFDAEVVPVQVPQRKGDPVRFARDEGFRPETTRESLGKLRTL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELCLTPMASLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLTLDQMDLVEL
NEAFACQVLAVLKGWEWHDQDAIEQKLNVNGSGISLGHPIGATGVRILATLLHELQRRGGRYGLETMCIGGGQGIAAVFE
RY

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV) [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=403, Percent_Identity=40.4466501240695, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=37, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=35.7320099255583, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=35.5498721227622, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=31.4685314685315, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI194353979, Length=383, Percent_Identity=26.3707571801567, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=41.5422885572139, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787663, Length=406, Percent_Identity=44.0886699507389, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=42.6799007444169, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=40.5405405405405, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788683, Length=417, Percent_Identity=31.1750599520384, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=38.1909547738693, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=32.1782178217822, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=32.089552238806, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=30.3030303030303, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=26.1728395061728, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=34.5679012345679, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=33.4987593052109, Blast_Score=183, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24655093, Length=404, Percent_Identity=40.8415841584158, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=39.8496240601504, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=33.4170854271357, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.0754716981132, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 43082; Mature: 43082

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAAIVTPLRTPVGTFGGSLRPVPVEELAATAVRAVVERSGIDPARIDDVVFAQSYANS
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EVPCVGRWAALQAGLPVEVPGMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSN
CCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCC
IEYYTTDMRWGARSGNVRFFDRLDRGRERSQPVERFGKISGMIETAENLARDYGISREAA
CEEEEECCCCCCCCCCCHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DVFAARSHARAAAAWEAGRFDAEVVPVQVPQRKGDPVRFARDEGFRPETTRESLGKLRTL
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
MPNGTVTAGNASQQNDASAACLIVAEDKLAELCLTPMASLVGWAAAGCEPSHMGIGPVPA
CCCCCEECCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VKKLLARLNLTLDQMDLVELNEAFACQVLAVLKGWEWHDQDAIEQKLNVNGSGISLGHPI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCC
GATGVRILATLLHELQRRGGRYGLETMCIGGGQGIAAVFERY
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MRRAAIVTPLRTPVGTFGGSLRPVPVEELAATAVRAVVERSGIDPARIDDVVFAQSYANS
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EVPCVGRWAALQAGLPVEVPGMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSN
CCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCC
IEYYTTDMRWGARSGNVRFFDRLDRGRERSQPVERFGKISGMIETAENLARDYGISREAA
CEEEEECCCCCCCCCCCHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DVFAARSHARAAAAWEAGRFDAEVVPVQVPQRKGDPVRFARDEGFRPETTRESLGKLRTL
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
MPNGTVTAGNASQQNDASAACLIVAEDKLAELCLTPMASLVGWAAAGCEPSHMGIGPVPA
CCCCCEECCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VKKLLARLNLTLDQMDLVELNEAFACQVLAVLKGWEWHDQDAIEQKLNVNGSGISLGHPI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCC
GATGVRILATLLHELQRRGGRYGLETMCIGGGQGIAAVFERY
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049 [H]