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The map label for this gene is hpaI2 [H]

Identifier: 116695125

GI number: 116695125

Start: 1334357

End: 1335166

Strand: Direct

Name: hpaI2 [H]

Synonym: H16_B1180

Alternate gene names: 116695125

Gene position: 1334357-1335166 (Clockwise)

Preceding gene: 116695124

Following gene: 116695126

Centisome position: 45.81

GC content: 68.77

Gene sequence:

>810_bases
ATGCCCGCAAACAATCCCTTCAAGACTGCGCTGGCCGCCCGCCAGGCGCAGATCGGCCTGTGGCTGTCGATGGCAACGCC
ATACCTGGCCGAAGTGTCCGCCACCGCAGGCTTTGACTGGCTGCTGATCGACGGCGAACACGCCCCCAACGACCTGCGCT
CGACGCTGCACGCGCTGCAGGCGGTGGCGCCATACCCGGTCCAGCCCGTGGTGCGTGCCGTCGCCGGTGAAGTGCCGCTG
ATCAAGCAGTTGCTCGACATCGGCGTGCGCAGCCTGCTCGTGCCGATGGTGGATACCGCGGAACAGGCGCGCATGGTGGT
CAGCGCTACGCGCTACCCCCCGCAAGGCATCCGCGGCGTGGGCAGCGCGATTGCGCGCGCCTCGCAGTGGAGCGCCCGCA
CCGACTACCTCGATGTGGCCGACGATGAAGTCTGCCTGCTGGTGCAGGCCGAAACCGTCACCGCATTGCAGAACCTGGAA
GCAATCTGCGCGGTGGATGGTATCGACGGCGTCTTTATCGGCCCGGCCGACCTGGCTGCGTCGATGGGCCACCGCGGCCG
CCCCGGTCACCCGGAGGTGCAGGCCGCCATCGAAGGCGCGATGCGCACCATCATTGCCAGCGGCAAGGCCGCCGGCACGC
TGACGTCGGACCCGGCACTGGCACGCCGCTACCTGGACCTGGGCTGCACCTTCGTGGCCACGGGCGTGGACGTGATGCTG
TATGCCAACGCCGCGCGCAAGCTTGCCGCATCGTTCCGCGAGCAGCCCTCTGACGCTCCCGCCGCAGACAAGCCTTCCGC
CGCGTACTGA

Upstream 100 bases:

>100_bases
TCGCGCCACGGGCTGGACGACTACATGCATACCAAGTACCTTTGCCAGGGTCAGCTCGGCTGAAGCGCCGGGCCCATCGA
TCATCGAACCGGAACACACC

Downstream 100 bases:

>100_bases
CCCGTATCTCCTTCGCCTTACACCCATGCCAGCCGAAACCCCGACCCCAGACACCACCTTGCGCGCGATGCAGGCCATCG
TTGGTGCAAGCGCCTGCCGC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: KDR aldolase; 2-dehydro-3-deoxyrhamnonate aldolase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPANNPFKTALAARQAQIGLWLSMATPYLAEVSATAGFDWLLIDGEHAPNDLRSTLHALQAVAPYPVQPVVRAVAGEVPL
IKQLLDIGVRSLLVPMVDTAEQARMVVSATRYPPQGIRGVGSAIARASQWSARTDYLDVADDEVCLLVQAETVTALQNLE
AICAVDGIDGVFIGPADLAASMGHRGRPGHPEVQAAIEGAMRTIIASGKAAGTLTSDPALARRYLDLGCTFVATGVDVML
YANAARKLAASFREQPSDAPAADKPSAAY

Sequences:

>Translated_269_residues
MPANNPFKTALAARQAQIGLWLSMATPYLAEVSATAGFDWLLIDGEHAPNDLRSTLHALQAVAPYPVQPVVRAVAGEVPL
IKQLLDIGVRSLLVPMVDTAEQARMVVSATRYPPQGIRGVGSAIARASQWSARTDYLDVADDEVCLLVQAETVTALQNLE
AICAVDGIDGVFIGPADLAASMGHRGRPGHPEVQAAIEGAMRTIIASGKAAGTLTSDPALARRYLDLGCTFVATGVDVML
YANAARKLAASFREQPSDAPAADKPSAAY
>Mature_268_residues
PANNPFKTALAARQAQIGLWLSMATPYLAEVSATAGFDWLLIDGEHAPNDLRSTLHALQAVAPYPVQPVVRAVAGEVPLI
KQLLDIGVRSLLVPMVDTAEQARMVVSATRYPPQGIRGVGSAIARASQWSARTDYLDVADDEVCLLVQAETVTALQNLEA
ICAVDGIDGVFIGPADLAASMGHRGRPGHPEVQAAIEGAMRTIIASGKAAGTLTSDPALARRYLDLGCTFVATGVDVMLY
ANAARKLAASFREQPSDAPAADKPSAAY

Specific function: Catalyzes the reversible retro-aldol cleavage of 2-keto- 3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=58.8, Blast_Score=300, Evalue=6e-83,
Organism=Escherichia coli, GI1789514, Length=250, Percent_Identity=46, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28257; Mature: 28125

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANNPFKTALAARQAQIGLWLSMATPYLAEVSATAGFDWLLIDGEHAPNDLRSTLHALQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
AVAPYPVQPVVRAVAGEVPLIKQLLDIGVRSLLVPMVDTAEQARMVVSATRYPPQGIRGV
HCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
GSAIARASQWSARTDYLDVADDEVCLLVQAETVTALQNLEAICAVDGIDGVFIGPADLAA
HHHHHHHHHCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHH
SMGHRGRPGHPEVQAAIEGAMRTIIASGKAAGTLTSDPALARRYLDLGCTFVATGVDVML
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCHHHEE
YANAARKLAASFREQPSDAPAADKPSAAY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PANNPFKTALAARQAQIGLWLSMATPYLAEVSATAGFDWLLIDGEHAPNDLRSTLHALQ
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
AVAPYPVQPVVRAVAGEVPLIKQLLDIGVRSLLVPMVDTAEQARMVVSATRYPPQGIRGV
HCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
GSAIARASQWSARTDYLDVADDEVCLLVQAETVTALQNLEAICAVDGIDGVFIGPADLAA
HHHHHHHHHCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHH
SMGHRGRPGHPEVQAAIEGAMRTIIASGKAAGTLTSDPALARRYLDLGCTFVATGVDVML
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCHHHEE
YANAARKLAASFREQPSDAPAADKPSAAY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA