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The map label for this gene is mhpA [H]

Identifier: 116694916

GI number: 116694916

Start: 1093314

End: 1094972

Strand: Direct

Name: mhpA [H]

Synonym: H16_B0971

Alternate gene names: 116694916

Gene position: 1093314-1094972 (Clockwise)

Preceding gene: 116694915

Following gene: 116694917

Centisome position: 37.54

GC content: 64.56

Gene sequence:

>1659_bases
ATGAGCATCAATCCGAAGCAAACACCCGAGCCGGCCGCAGAAATCTTCGACGTAGCCGTGATCGGATATGGCCCGACCGG
ACTCGTCGCGGCCTCGCTGCTGGGCGAGGCTGGCTACAAGGTGGTTGTCATTGAACGCTGGCCCACGCCTTATGGCCTGC
CGCGCCTGACCCATATCGATGGCGAGACGGCACGGATCGTCCAGGCGTGCGGAGACGTTGACCAGGCCCTGCGCGATGCC
TGTGGGCTGGGTCCGTATCAGTATCGCAACGCCGACGATGAGCTCCTCATTGAACTGGACTGGACCGGGCACGCGTGCGG
CTTCCCGGCCCATATCTCGATTTACCAGCCCGATATCGAAGACGCCATTCATCAGCGCGCGGCAAGCTTTCCCGGGGTCG
AGATCCTTCGCGGCTGGGAGGCAGAGACGCTCAGGCAGGACGAGGATGGCGTCAGGATCTCCGCGCACCTCTGGCGCGGC
GCGCAGGACTCGCAATGGACGACACCGTCGCGCGAGTTCATCGCAAAGTACGTCATTGGCGCCGACGGGGCGAACAGCTT
TGTCCGCCGCAGTCTGTGCATCGAACGCGACGACTTCGGGCAGAACGAGCGCTGGTTGAACCTTGACTCGGAGAACAAGC
GGGACCTTGGCGAGAAGTTCTCGCTCACGACCATCTATTGCGACCCGGCGCGGGCCCATATGTACATGCCGATCGGCACC
CGGCGGACTCGCTTCGAACTGCGGGTGCTGCCTGGCGAGGATACTGCCTACTGGGAGGACGAGGCCGTCGGCTGGCGCTG
GCTGAAGGAGCAGCATGGCCTGGGCCCGGAAGACATGCGCCTGTTGCGCCATGTCGTCTACACATTCGAAACCCGCATGG
CGCACCGGTGGCGCGACGGGCGGATCTTCCTGGCCGGCGATGCCGCGCATACGATGATGCCCTATATGGGGCAAGGCGCG
TGTTCGGGCATGCGAGACGGCATCAACCTGGCCTGGAAGCTGGACCTGGTGCTCAGTGGCCGAAGCGCGCCGGGTCTGCT
GGATACGTATGAAGAAGAGCGCAGGCCCCACGTCAGCAAGATCATGGAGATGGCGAGATTCCTTGGCAAGGTTGCCAACG
AAGACGATCCGCAAAAGGTTGCCGAGCGCGACGCCGCATTCCGGGCCCGGTCGATCCCCCCGATGCCGCCGTTTCCGAGG
CTCGAAGCCGGCATCGTGCATGCCGAGGTGGATGGCAGCCTGTTGCCCGCGATCGGCACGCCCGCTCCGCAGGGACGCGT
GCGCAAGGGGGAAGTCGAAGGGCGCCTCGATGACGTGGTCGGTCATGGCTTCCTGCTGATCGCTCTTGACGATCCGTTGC
GCTACCTCGATGAAGGCCAGCTCGGTTTCCTTGAGCAACTCGGCTGCCATATCGTCGTGCTGTCCGACGCGGCGTCGATA
CCCGGGGCAGTGGTGGAACTCAATGGCGCGCAGGCAGCGTTCATGCGCGCGCATGGCATGCAGGCTTACATCAGCCGGCC
GGACTTTGCTGTCTTCGGCGGCGTTTCCGACCTGGCGCATCTGCCCGGCCTGGTCGATTCCCTGCGCCAGAAGCTGCACT
GGCGCGGTGCGGCGCAATCCAGCATGCTGGCCGGGACCAGCGCAGCGGCTGTTTCGTGA

Upstream 100 bases:

>100_bases
CTGCTGCAGGACGCCTTCGACGGAACGCGTCCGTAATGCGCAACTCGCCGAGCTGCCACGGGACAGCGTCCCCCAAAACC
AATATAGATGGAGACAAAGC

Downstream 100 bases:

>100_bases
GCGCGGGAGCCATGATGACGACAACGGTTTCTTCGGCAGCGCTTGCGTTCCAGCGGGCGATGCCGGTCACACGCTTGCTG
GATTGCGGCATGGACTATGC

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: tetrachlorohydroquinone; NADP+; chloride

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSINPKQTPEPAAEIFDVAVIGYGPTGLVAASLLGEAGYKVVVIERWPTPYGLPRLTHIDGETARIVQACGDVDQALRDA
CGLGPYQYRNADDELLIELDWTGHACGFPAHISIYQPDIEDAIHQRAASFPGVEILRGWEAETLRQDEDGVRISAHLWRG
AQDSQWTTPSREFIAKYVIGADGANSFVRRSLCIERDDFGQNERWLNLDSENKRDLGEKFSLTTIYCDPARAHMYMPIGT
RRTRFELRVLPGEDTAYWEDEAVGWRWLKEQHGLGPEDMRLLRHVVYTFETRMAHRWRDGRIFLAGDAAHTMMPYMGQGA
CSGMRDGINLAWKLDLVLSGRSAPGLLDTYEEERRPHVSKIMEMARFLGKVANEDDPQKVAERDAAFRARSIPPMPPFPR
LEAGIVHAEVDGSLLPAIGTPAPQGRVRKGEVEGRLDDVVGHGFLLIALDDPLRYLDEGQLGFLEQLGCHIVVLSDAASI
PGAVVELNGAQAAFMRAHGMQAYISRPDFAVFGGVSDLAHLPGLVDSLRQKLHWRGAAQSSMLAGTSAAAVS

Sequences:

>Translated_552_residues
MSINPKQTPEPAAEIFDVAVIGYGPTGLVAASLLGEAGYKVVVIERWPTPYGLPRLTHIDGETARIVQACGDVDQALRDA
CGLGPYQYRNADDELLIELDWTGHACGFPAHISIYQPDIEDAIHQRAASFPGVEILRGWEAETLRQDEDGVRISAHLWRG
AQDSQWTTPSREFIAKYVIGADGANSFVRRSLCIERDDFGQNERWLNLDSENKRDLGEKFSLTTIYCDPARAHMYMPIGT
RRTRFELRVLPGEDTAYWEDEAVGWRWLKEQHGLGPEDMRLLRHVVYTFETRMAHRWRDGRIFLAGDAAHTMMPYMGQGA
CSGMRDGINLAWKLDLVLSGRSAPGLLDTYEEERRPHVSKIMEMARFLGKVANEDDPQKVAERDAAFRARSIPPMPPFPR
LEAGIVHAEVDGSLLPAIGTPAPQGRVRKGEVEGRLDDVVGHGFLLIALDDPLRYLDEGQLGFLEQLGCHIVVLSDAASI
PGAVVELNGAQAAFMRAHGMQAYISRPDFAVFGGVSDLAHLPGLVDSLRQKLHWRGAAQSSMLAGTSAAAVS
>Mature_551_residues
SINPKQTPEPAAEIFDVAVIGYGPTGLVAASLLGEAGYKVVVIERWPTPYGLPRLTHIDGETARIVQACGDVDQALRDAC
GLGPYQYRNADDELLIELDWTGHACGFPAHISIYQPDIEDAIHQRAASFPGVEILRGWEAETLRQDEDGVRISAHLWRGA
QDSQWTTPSREFIAKYVIGADGANSFVRRSLCIERDDFGQNERWLNLDSENKRDLGEKFSLTTIYCDPARAHMYMPIGTR
RTRFELRVLPGEDTAYWEDEAVGWRWLKEQHGLGPEDMRLLRHVVYTFETRMAHRWRDGRIFLAGDAAHTMMPYMGQGAC
SGMRDGINLAWKLDLVLSGRSAPGLLDTYEEERRPHVSKIMEMARFLGKVANEDDPQKVAERDAAFRARSIPPMPPFPRL
EAGIVHAEVDGSLLPAIGTPAPQGRVRKGEVEGRLDDVVGHGFLLIALDDPLRYLDEGQLGFLEQLGCHIVVLSDAASIP
GAVVELNGAQAAFMRAHGMQAYISRPDFAVFGGVSDLAHLPGLVDSLRQKLHWRGAAQSSMLAGTSAAAVS

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=516, Percent_Identity=29.0697674418605, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.50

Molecular weight: Translated: 61014; Mature: 60883

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINPKQTPEPAAEIFDVAVIGYGPTGLVAASLLGEAGYKVVVIERWPTPYGLPRLTHID
CCCCCCCCCCHHHHHEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
GETARIVQACGDVDQALRDACGLGPYQYRNADDELLIELDWTGHACGFPAHISIYQPDIE
CHHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCCCCCCCEEEEECCCHH
DAIHQRAASFPGVEILRGWEAETLRQDEDGVRISAHLWRGAQDSQWTTPSREFIAKYVIG
HHHHHHHHCCCCHHHHCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
ADGANSFVRRSLCIERDDFGQNERWLNLDSENKRDLGEKFSLTTIYCDPARAHMYMPIGT
CCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCC
RRTRFELRVLPGEDTAYWEDEAVGWRWLKEQHGLGPEDMRLLRHVVYTFETRMAHRWRDG
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RIFLAGDAAHTMMPYMGQGACSGMRDGINLAWKLDLVLSGRSAPGLLDTYEEERRPHVSK
EEEEECCHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCHHHH
IMEMARFLGKVANEDDPQKVAERDAAFRARSIPPMPPFPRLEAGIVHAEVDGSLLPAIGT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCC
PAPQGRVRKGEVEGRLDDVVGHGFLLIALDDPLRYLDEGQLGFLEQLGCHIVVLSDAASI
CCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCHHHHCCCCCCHHHHCCCEEEEEECCCCC
PGAVVELNGAQAAFMRAHGMQAYISRPDFAVFGGVSDLAHLPGLVDSLRQKLHWRGAAQS
CCEEEEECCCHHHHHHHCCCHHHHCCCCEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCC
SMLAGTSAAAVS
HHHCCCCHHCCC
>Mature Secondary Structure 
SINPKQTPEPAAEIFDVAVIGYGPTGLVAASLLGEAGYKVVVIERWPTPYGLPRLTHID
CCCCCCCCCHHHHHEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
GETARIVQACGDVDQALRDACGLGPYQYRNADDELLIELDWTGHACGFPAHISIYQPDIE
CHHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCCCCCCCEEEEECCCHH
DAIHQRAASFPGVEILRGWEAETLRQDEDGVRISAHLWRGAQDSQWTTPSREFIAKYVIG
HHHHHHHHCCCCHHHHCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
ADGANSFVRRSLCIERDDFGQNERWLNLDSENKRDLGEKFSLTTIYCDPARAHMYMPIGT
CCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCC
RRTRFELRVLPGEDTAYWEDEAVGWRWLKEQHGLGPEDMRLLRHVVYTFETRMAHRWRDG
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RIFLAGDAAHTMMPYMGQGACSGMRDGINLAWKLDLVLSGRSAPGLLDTYEEERRPHVSK
EEEEECCHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCHHHH
IMEMARFLGKVANEDDPQKVAERDAAFRARSIPPMPPFPRLEAGIVHAEVDGSLLPAIGT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCC
PAPQGRVRKGEVEGRLDDVVGHGFLLIALDDPLRYLDEGQLGFLEQLGCHIVVLSDAASI
CCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCHHHHCCCCCCHHHHCCCEEEEEECCCCC
PGAVVELNGAQAAFMRAHGMQAYISRPDFAVFGGVSDLAHLPGLVDSLRQKLHWRGAAQS
CCEEEEECCCHHHHHHHCCCHHHHCCCCEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCC
SMLAGTSAAAVS
HHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pentachlorophenol; NADPH; H+; O2

Specific reaction: pentachlorophenol + NADPH + H+ + O2 = tetrachlorohydroquinone + NADP+ + chloride

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA