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The map label for this gene is nasD [H]

Identifier: 116694893

GI number: 116694893

Start: 1070065

End: 1072035

Strand: Direct

Name: nasD [H]

Synonym: H16_B0948

Alternate gene names: 116694893

Gene position: 1070065-1072035 (Clockwise)

Preceding gene: 116694892

Following gene: 116694894

Centisome position: 36.74

GC content: 64.28

Gene sequence:

>1971_bases
ATGCTGCAATCGATACCAAACACAAAGGCTGTCCAAGCGCCGGTCGTCGTGATCGGCACCGGGCCGGTGGGCGTGCGGCT
TGCGCAGGACTTGCATCGTCGCGATCCTTCCATTCCGCTGGTGATCTACGGCAACGAACCATGGGAGCCATACAACCGCG
TGCGCCTGTCTTCCTTTCTTGCCGGCGATGCGAGCTGGGCCACGCTCACGCAGGGGCTTGCACTGCCGCAGGGACCCGCG
ATCGCAGCGCGGCTCAATTGCGCAGTGGTGCATGTCGATCGCGGCGCGCAGGAGATTGAAGACTCGGCCGGGCGCACGCA
ACGCTATTCCCACTTAGTGCTGGCCACCGGATCACGTCCGCGGATCCCGGACGTGCCGGGCATCGGCCAGGCCAATGTCT
ACGTTTTCCGTGACATGAACGATACCCAGCGGCTGCTCGCACGCCGTATCCGCAGCCGCCGTACGGTGGTGTTGGGCGGG
GGGCTGCTGGGCCTGGAAGCGGCGCGCGCAATGCAGCGCTTCAACACCGAGGTGCTGGTCATCGAGAACAACGACCGGCT
GATGATGCAGCAATTGGACCAGGCCGCCGCGGATCTGCTGCGCGCCCATGTCGAGGCGGCCGGAATCCAGGTATTGACGG
GCGCCGCAGTGAAACGCGTGCATGGTGAAGGAGCGGTTACCGGCATCACCCTGCGCACCGGCGATGTCATCGAATGCGAT
ACCGTCGTGATTGCCGCCGGCATCAAGCCGAACACCGACCTGGCCCTGCAGGCCGGCCTGAATGTGGGGCGCGGCATTCG
CGTCAGCGACCGCATGCAGACGTCCGATCCACATATCTACGCCGTTGGCGAATGCGCGGAACATCGCGAACAAGTGTACG
GGATCGTCGCCCCTGGCCTGGAACAGGCGGCAGTCGCCGTGCATGCAATATGCGGCGGCGACGCCAGCTATGCCGGTTCG
CTACAGGCAACGCGCCTGAAAGTGCTCGATATGCCGGTGTTCAGCGTGGGCCGCGTCGGCGAGCATGACGGTATCGGCTC
GGCGAGCACGGCGGCTTATGAAGACAAAAAAACTGGCATCTACCGGAAGGTGCTGGTCGAGCGCGGTCATCTGATTGGCG
CGATGGCGGTCGGCGAAGGCGCAGACCTGCGTCGGCTGCAGGACGCGGTCAGCGGCAATCACCGGATACTGCCATGGCAG
CTTTGGCGGTTTCGCAGGACCGGCTCGCTGTGGCAGGAGGGCGAAGCCAGCGTCGTCAACTGGCCGGCAAGCGCGGTCGT
CTGCAACTGCACCGGCGTTACCCGCGGTCAGCTTGGCGAAGCGGCGGCTGCCGGGTGCCGTTCGATCGAGCAACTCGCAG
CGTGCACGCGTGCCTCTACGGTATGCGGTTCGTGCAAGCCGTTGCTGGCCGAGTTGGCCGGTGGCGCGGTGATGCGAGAA
CCGGTGCGCGCGCATCGTCCGCTCCAGGGCGCGGCCGCGGCGGCACTGGCGCTCGCCTTGCTGGCACTCGTTGCGCCTGC
CATCCCCTTTGCGAAGACGGTGCAGCTGCAGTGGCGCTGGGACGAGCTATGGCTGGTGAGTTTGTATAAGCAGGTCAGCG
GATTTTCCATTCTCGGGTTGAGCGTACTTGCCCTGCTTCTCTCATTCCGGAAGCGCTGGTCGCGCCTTGGCTATGGCGAT
TTTGCCATCTGGCGCGTGCTGCATGTGGTGCTCGGATTGGGTGTGCTGGCGGGACTCTATGCGCATACGGGAGGCAGGCT
TGGCAGCCAGCTGAATTTCCTGTTGATGGCGAGTTTCACCGGCCTGATTCTGGCGGGCAGCATGTCGGGGCTGATGATCG
CGCAAGGGCATCGCCTGCCAGTGGCACTGGTTCGGCGCTGGCGCGACAACAGCACCTGGGTGCACATCTTGCTGTTCTGG
CCGGTACCCGTGCTGCTCGGGTTCCACATTCTCAAAACTTACTATTTCTAG

Upstream 100 bases:

>100_bases
CCGGGACAAAGGCGTCCGAGTCAGGCGAGCGGTGCTCGCTGGGCCGGATGCATTTTTTTTGTCTGTAAGTGGTGCACAGG
GGGAGTCAGGGGTGTCAGTG

Downstream 100 bases:

>100_bases
AGCCTACCAGCGATGGCAAGCAAAAGCCGCAGAGTTCTGTGGGTCTACTGGGGTGTGTTGAGCGTGGCGATGCTGGCATA
TTTCTCGATTGGGCTGTTCT

Product: NAD(FAD)-dependent dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MLQSIPNTKAVQAPVVVIGTGPVGVRLAQDLHRRDPSIPLVIYGNEPWEPYNRVRLSSFLAGDASWATLTQGLALPQGPA
IAARLNCAVVHVDRGAQEIEDSAGRTQRYSHLVLATGSRPRIPDVPGIGQANVYVFRDMNDTQRLLARRIRSRRTVVLGG
GLLGLEAARAMQRFNTEVLVIENNDRLMMQQLDQAAADLLRAHVEAAGIQVLTGAAVKRVHGEGAVTGITLRTGDVIECD
TVVIAAGIKPNTDLALQAGLNVGRGIRVSDRMQTSDPHIYAVGECAEHREQVYGIVAPGLEQAAVAVHAICGGDASYAGS
LQATRLKVLDMPVFSVGRVGEHDGIGSASTAAYEDKKTGIYRKVLVERGHLIGAMAVGEGADLRRLQDAVSGNHRILPWQ
LWRFRRTGSLWQEGEASVVNWPASAVVCNCTGVTRGQLGEAAAAGCRSIEQLAACTRASTVCGSCKPLLAELAGGAVMRE
PVRAHRPLQGAAAAALALALLALVAPAIPFAKTVQLQWRWDELWLVSLYKQVSGFSILGLSVLALLLSFRKRWSRLGYGD
FAIWRVLHVVLGLGVLAGLYAHTGGRLGSQLNFLLMASFTGLILAGSMSGLMIAQGHRLPVALVRRWRDNSTWVHILLFW
PVPVLLGFHILKTYYF

Sequences:

>Translated_656_residues
MLQSIPNTKAVQAPVVVIGTGPVGVRLAQDLHRRDPSIPLVIYGNEPWEPYNRVRLSSFLAGDASWATLTQGLALPQGPA
IAARLNCAVVHVDRGAQEIEDSAGRTQRYSHLVLATGSRPRIPDVPGIGQANVYVFRDMNDTQRLLARRIRSRRTVVLGG
GLLGLEAARAMQRFNTEVLVIENNDRLMMQQLDQAAADLLRAHVEAAGIQVLTGAAVKRVHGEGAVTGITLRTGDVIECD
TVVIAAGIKPNTDLALQAGLNVGRGIRVSDRMQTSDPHIYAVGECAEHREQVYGIVAPGLEQAAVAVHAICGGDASYAGS
LQATRLKVLDMPVFSVGRVGEHDGIGSASTAAYEDKKTGIYRKVLVERGHLIGAMAVGEGADLRRLQDAVSGNHRILPWQ
LWRFRRTGSLWQEGEASVVNWPASAVVCNCTGVTRGQLGEAAAAGCRSIEQLAACTRASTVCGSCKPLLAELAGGAVMRE
PVRAHRPLQGAAAAALALALLALVAPAIPFAKTVQLQWRWDELWLVSLYKQVSGFSILGLSVLALLLSFRKRWSRLGYGD
FAIWRVLHVVLGLGVLAGLYAHTGGRLGSQLNFLLMASFTGLILAGSMSGLMIAQGHRLPVALVRRWRDNSTWVHILLFW
PVPVLLGFHILKTYYF
>Mature_656_residues
MLQSIPNTKAVQAPVVVIGTGPVGVRLAQDLHRRDPSIPLVIYGNEPWEPYNRVRLSSFLAGDASWATLTQGLALPQGPA
IAARLNCAVVHVDRGAQEIEDSAGRTQRYSHLVLATGSRPRIPDVPGIGQANVYVFRDMNDTQRLLARRIRSRRTVVLGG
GLLGLEAARAMQRFNTEVLVIENNDRLMMQQLDQAAADLLRAHVEAAGIQVLTGAAVKRVHGEGAVTGITLRTGDVIECD
TVVIAAGIKPNTDLALQAGLNVGRGIRVSDRMQTSDPHIYAVGECAEHREQVYGIVAPGLEQAAVAVHAICGGDASYAGS
LQATRLKVLDMPVFSVGRVGEHDGIGSASTAAYEDKKTGIYRKVLVERGHLIGAMAVGEGADLRRLQDAVSGNHRILPWQ
LWRFRRTGSLWQEGEASVVNWPASAVVCNCTGVTRGQLGEAAAAGCRSIEQLAACTRASTVCGSCKPLLAELAGGAVMRE
PVRAHRPLQGAAAAALALALLALVAPAIPFAKTVQLQWRWDELWLVSLYKQVSGFSILGLSVLALLLSFRKRWSRLGYGD
FAIWRVLHVVLGLGVLAGLYAHTGGRLGSQLNFLLMASFTGLILAGSMSGLMIAQGHRLPVALVRRWRDNSTWVHILLFW
PVPVLLGFHILKTYYF

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=28.4172661870504, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=28.4172661870504, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=28.4172661870504, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI4757732, Length=325, Percent_Identity=25.5384615384615, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI22202629, Length=325, Percent_Identity=25.5384615384615, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789765, Length=488, Percent_Identity=33.8114754098361, Blast_Score=215, Evalue=9e-57,
Organism=Escherichia coli, GI1789065, Length=336, Percent_Identity=26.4880952380952, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788892, Length=277, Percent_Identity=25.2707581227437, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082354, Length=228, Percent_Identity=27.1929824561404, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=291, Percent_Identity=25.4295532646048, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585130, Length=285, Percent_Identity=23.1578947368421, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639257, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281359715, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281359713, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24639250, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18543267, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24639252, Length=290, Percent_Identity=22.7586206896552, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 70629; Mature: 70629

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSIPNTKAVQAPVVVIGTGPVGVRLAQDLHRRDPSIPLVIYGNEPWEPYNRVRLSSFL
CCCCCCCCCCEECCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
AGDASWATLTQGLALPQGPAIAARLNCAVVHVDRGAQEIEDSAGRTQRYSHLVLATGSRP
CCCCCHHHHHHCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHCCCHHHCEEEEEEECCCC
RIPDVPGIGQANVYVFRDMNDTQRLLARRIRSRRTVVLGGGLLGLEAARAMQRFNTEVLV
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEEE
IENNDRLMMQQLDQAAADLLRAHVEAAGIQVLTGAAVKRVHGEGAVTGITLRTGDVIECD
EECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEECCCEEEEC
TVVIAAGIKPNTDLALQAGLNVGRGIRVSDRMQTSDPHIYAVGECAEHREQVYGIVAPGL
EEEEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCCCEEEECCHHHHHHHEEEEECCCH
EQAAVAVHAICGGDASYAGSLQATRLKVLDMPVFSVGRVGEHDGIGSASTAAYEDKKTGI
HHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCHHCCCCCCCCCCCCCCCCCCCCHHHHH
YRKVLVERGHLIGAMAVGEGADLRRLQDAVSGNHRILPWQLWRFRRTGSLWQEGEASVVN
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCEEE
WPASAVVCNCTGVTRGQLGEAAAAGCRSIEQLAACTRASTVCGSCKPLLAELAGGAVMRE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHC
PVRAHRPLQGAAAAALALALLALVAPAIPFAKTVQLQWRWDELWLVSLYKQVSGFSILGL
CHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCHHHH
SVLALLLSFRKRWSRLGYGDFAIWRVLHVVLGLGVLAGLYAHTGGRLGSQLNFLLMASFT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GLILAGSMSGLMIAQGHRLPVALVRRWRDNSTWVHILLFWPVPVLLGFHILKTYYF
HHHEECCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQSIPNTKAVQAPVVVIGTGPVGVRLAQDLHRRDPSIPLVIYGNEPWEPYNRVRLSSFL
CCCCCCCCCCEECCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
AGDASWATLTQGLALPQGPAIAARLNCAVVHVDRGAQEIEDSAGRTQRYSHLVLATGSRP
CCCCCHHHHHHCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHCCCHHHCEEEEEEECCCC
RIPDVPGIGQANVYVFRDMNDTQRLLARRIRSRRTVVLGGGLLGLEAARAMQRFNTEVLV
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCEEEE
IENNDRLMMQQLDQAAADLLRAHVEAAGIQVLTGAAVKRVHGEGAVTGITLRTGDVIECD
EECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEECCCEEEEC
TVVIAAGIKPNTDLALQAGLNVGRGIRVSDRMQTSDPHIYAVGECAEHREQVYGIVAPGL
EEEEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCCCEEEECCHHHHHHHEEEEECCCH
EQAAVAVHAICGGDASYAGSLQATRLKVLDMPVFSVGRVGEHDGIGSASTAAYEDKKTGI
HHHHHHHHHHCCCCCCCCCCCHHEEEEEECCCCHHCCCCCCCCCCCCCCCCCCCCHHHHH
YRKVLVERGHLIGAMAVGEGADLRRLQDAVSGNHRILPWQLWRFRRTGSLWQEGEASVVN
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCEEE
WPASAVVCNCTGVTRGQLGEAAAAGCRSIEQLAACTRASTVCGSCKPLLAELAGGAVMRE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHC
PVRAHRPLQGAAAAALALALLALVAPAIPFAKTVQLQWRWDELWLVSLYKQVSGFSILGL
CHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCHHHH
SVLALLLSFRKRWSRLGYGDFAIWRVLHVVLGLGVLAGLYAHTGGRLGSQLNFLLMASFT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GLILAGSMSGLMIAQGHRLPVALVRRWRDNSTWVHILLFWPVPVLLGFHILKTYYF
HHHEECCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]