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The map label for this gene is caiD [H]

Identifier: 116694794

GI number: 116694794

Start: 961906

End: 962595

Strand: Direct

Name: caiD [H]

Synonym: H16_B0848

Alternate gene names: 116694794

Gene position: 961906-962595 (Clockwise)

Preceding gene: 116694793

Following gene: 116694795

Centisome position: 33.03

GC content: 73.19

Gene sequence:

>690_bases
ATGAACACCCCCCAATCCCCTGTACTGCTGCAGCGCCATGCCGGCTGGGCCGAGGTGGTGCTGAACCGCCCGGCCCGCCG
CAATGCCGTCGACATGTCGCTGGCACGCGCGCTGGGCGACGCCATCGAGACCCTGGCCGCCGACGACAGCGTGCGCGCCG
TGCTGCTGCGCGGCGCCGACGGCGCCTTCTGTTCCGGCCTGGACCTGCAGGCACTGCAGGCGGAGCGCGGCGGGCTGGCC
GCGTTTGCGCCGCTCTGGGAGCGCGTCCACCTGGGCCTGCTGCAAAGCAGCAAGGCATGGATCGTGGCGCTGGAGCGCCA
TGCCATCAACGGCGGCGCGGCGCTGGCGCTGGCCGGCGACCTGCTGGTGTGCGGCGCCGGGGCCTTCCTGCAGATCGGCG
AGATCCGCCTGGGCATGGCCGCGCCGCGCAATGCCGACTGGCTGGCGCTGCGCCATTCCGAGGCCGTGGCCGCGCGCCTG
TGCCTGCTTGGCGACCGCGTGCCCGCGCCGGAGCTGCTGCGCCTGGGCATTGCCACCGAACTGGTCGAAGACGCCGAGGT
GCTGGACCGCGCGCGCGGCCTGGCCGCCACCATCGGGGGTTTTCCGGCTGATGGCGTGGCTGCGATCAAGGGCGGCATGC
GCGCCGCGACGAGGCGCATGGCATCTGCTGCGCGCGACAAGAATTCCTGA

Upstream 100 bases:

>100_bases
CGACCGAGGTGCTGGCCGAGCTCGGCTTCAGCGCCGCGGAGATTCACACCATGGCCCGCGCCGGCGCGATCGGCGTGGAA
CAAGCCCGGCAGGAGCCCGC

Downstream 100 bases:

>100_bases
GGACCGATATTTCCATGACAGATTCCATCACCACCGACGACGACGCGTCGCTGCAGGACTTCCGCCGCGAGGTGCGCGAG
TTCGTCGCCCGGTCGCTGCC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNTPQSPVLLQRHAGWAEVVLNRPARRNAVDMSLARALGDAIETLAADDSVRAVLLRGADGAFCSGLDLQALQAERGGLA
AFAPLWERVHLGLLQSSKAWIVALERHAINGGAALALAGDLLVCGAGAFLQIGEIRLGMAAPRNADWLALRHSEAVAARL
CLLGDRVPAPELLRLGIATELVEDAEVLDRARGLAATIGGFPADGVAAIKGGMRAATRRMASAARDKNS

Sequences:

>Translated_229_residues
MNTPQSPVLLQRHAGWAEVVLNRPARRNAVDMSLARALGDAIETLAADDSVRAVLLRGADGAFCSGLDLQALQAERGGLA
AFAPLWERVHLGLLQSSKAWIVALERHAINGGAALALAGDLLVCGAGAFLQIGEIRLGMAAPRNADWLALRHSEAVAARL
CLLGDRVPAPELLRLGIATELVEDAEVLDRARGLAATIGGFPADGVAAIKGGMRAATRRMASAARDKNS
>Mature_229_residues
MNTPQSPVLLQRHAGWAEVVLNRPARRNAVDMSLARALGDAIETLAADDSVRAVLLRGADGAFCSGLDLQALQAERGGLA
AFAPLWERVHLGLLQSSKAWIVALERHAINGGAALALAGDLLVCGAGAFLQIGEIRLGMAAPRNADWLALRHSEAVAARL
CLLGDRVPAPELLRLGIATELVEDAEVLDRARGLAATIGGFPADGVAAIKGGMRAATRRMASAARDKNS

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI221142681, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=31.1320754716981, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653477, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 23916; Mature: 23916

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPQSPVLLQRHAGWAEVVLNRPARRNAVDMSLARALGDAIETLAADDSVRAVLLRGAD
CCCCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GAFCSGLDLQALQAERGGLAAFAPLWERVHLGLLQSSKAWIVALERHAINGGAALALAGD
CCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHCCCCCEEEEECCC
LLVCGAGAFLQIGEIRLGMAAPRNADWLALRHSEAVAARLCLLGDRVPAPELLRLGIATE
EEEECCCCCEEECCEEECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVEDAEVLDRARGLAATIGGFPADGVAAIKGGMRAATRRMASAARDKNS
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTPQSPVLLQRHAGWAEVVLNRPARRNAVDMSLARALGDAIETLAADDSVRAVLLRGAD
CCCCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GAFCSGLDLQALQAERGGLAAFAPLWERVHLGLLQSSKAWIVALERHAINGGAALALAGD
CCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHCCCCCEEEEECCC
LLVCGAGAFLQIGEIRLGMAAPRNADWLALRHSEAVAARLCLLGDRVPAPELLRLGIATE
EEEECCCCCEEECCEEECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVEDAEVLDRARGLAATIGGFPADGVAAIKGGMRAATRRMASAARDKNS
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA