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The map label for this gene is fucA [C]

Identifier: 116694786

GI number: 116694786

Start: 952117

End: 952887

Strand: Direct

Name: fucA [C]

Synonym: H16_B0840

Alternate gene names: 116694786

Gene position: 952117-952887 (Clockwise)

Preceding gene: 116694783

Following gene: 116694787

Centisome position: 32.69

GC content: 66.54

Gene sequence:

>771_bases
ATGGCGCAAATCCAGCTCGTCGATTCAATCAGGGACCGCGTCTCCGACGCCGAATGGCAGATGCGCGTGGACCTGGCGGC
CGCGTACCGCCTGGTCGCCCACTTCGGCTGGGACGACCTGATCTTCACCCATATCTCGGCACGCGTGCCCGATGCACCGG
ACCAGTTCCTGATCAACCCGTACGGGATGATGTTCGACGAGATCACGGCATCGAGCCTGGTCAAGGTCGACCACGAAGGG
CAGCCCGTGCTGGAAACGCCGTATGACGTCAACCCGGCCGGCTTCATCATCCACAGCGCCGTGCACGAGGCGCGCCCCGA
AGTCGGCTGCGTGATGCACACGCATACCTCGCACGGCGTCGCCGTCTCGGCGCAGCAGGCGGGCCTGCTGCCGATTTCGC
AGCAGTCGATGTTCGCGCTGACCGGGCTGGCGTACCACGACTATGAAGGCGTGGCGCTGCGCGAGGACGAAAAGGCGCGG
CTGGTGGCAGACCTGGGCCGCTGCAAGCAGATGATCCTGCGCAACCATGGCCTGCTGACGTGCGGGCGCACGGTATCCGA
TGCTTTCCTGACCATGTACACGCTCGAGTCCGCCTGCCGCATCCAGATCCTGGCGCAAAGCGGCGGCACGCCGCTGACCA
TGGTGCCCGAAGCCGCCAGCGCCAACATGGGCCAGCAGGCGCGCCAGGCCACCAAGGGCAAGGGCTCCAACCTGGCCTGG
CCGGGCCTGCTGCGCCGCCTCGACCGCATCAACCCTGACTACCGCAACTGA

Upstream 100 bases:

>100_bases
CTGCTGGAGCCGAGATTGTCCAATACTTAACAGACTTTGGCGCGGTGCGCCCGGACAATGCATCGATTCAATGACTCACA
TGCCCCCGGGAGGTACGCAT

Downstream 100 bases:

>100_bases
CCATGGCCATCCTCGTTCACCTGCCGTCGTTCATGGCGGTTCCCATCACTGCCGCGCTGCGCGAAGCCGCGCCGGACATC
ACCGTGTGGAACGGCCGCGA

Product: aldolase II superfamily protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAQIQLVDSIRDRVSDAEWQMRVDLAAAYRLVAHFGWDDLIFTHISARVPDAPDQFLINPYGMMFDEITASSLVKVDHEG
QPVLETPYDVNPAGFIIHSAVHEARPEVGCVMHTHTSHGVAVSAQQAGLLPISQQSMFALTGLAYHDYEGVALREDEKAR
LVADLGRCKQMILRNHGLLTCGRTVSDAFLTMYTLESACRIQILAQSGGTPLTMVPEAASANMGQQARQATKGKGSNLAW
PGLLRRLDRINPDYRN

Sequences:

>Translated_256_residues
MAQIQLVDSIRDRVSDAEWQMRVDLAAAYRLVAHFGWDDLIFTHISARVPDAPDQFLINPYGMMFDEITASSLVKVDHEG
QPVLETPYDVNPAGFIIHSAVHEARPEVGCVMHTHTSHGVAVSAQQAGLLPISQQSMFALTGLAYHDYEGVALREDEKAR
LVADLGRCKQMILRNHGLLTCGRTVSDAFLTMYTLESACRIQILAQSGGTPLTMVPEAASANMGQQARQATKGKGSNLAW
PGLLRRLDRINPDYRN
>Mature_255_residues
AQIQLVDSIRDRVSDAEWQMRVDLAAAYRLVAHFGWDDLIFTHISARVPDAPDQFLINPYGMMFDEITASSLVKVDHEGQ
PVLETPYDVNPAGFIIHSAVHEARPEVGCVMHTHTSHGVAVSAQQAGLLPISQQSMFALTGLAYHDYEGVALREDEKARL
VADLGRCKQMILRNHGLLTCGRTVSDAFLTMYTLESACRIQILAQSGGTPLTMVPEAASANMGQQARQATKGKGSNLAWP
GLLRRLDRINPDYRN

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826323, Length=249, Percent_Identity=42.9718875502008, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI29826325, Length=249, Percent_Identity=42.9718875502008, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI29826319, Length=249, Percent_Identity=42.9718875502008, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI29826321, Length=249, Percent_Identity=42.9718875502008, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI9951927, Length=250, Percent_Identity=38, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI62912453, Length=250, Percent_Identity=38, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI9943848, Length=250, Percent_Identity=38, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI89276787, Length=201, Percent_Identity=39.3034825870647, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI297307160, Length=201, Percent_Identity=39.3034825870647, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI9257190, Length=201, Percent_Identity=39.3034825870647, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI297307158, Length=201, Percent_Identity=39.3034825870647, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI9257192, Length=201, Percent_Identity=39.3034825870647, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115533216, Length=248, Percent_Identity=41.9354838709677, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI115533218, Length=248, Percent_Identity=41.9354838709677, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71989541, Length=252, Percent_Identity=32.5396825396825, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71989550, Length=252, Percent_Identity=32.5396825396825, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71989560, Length=252, Percent_Identity=32.5396825396825, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71989555, Length=252, Percent_Identity=32.5396825396825, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71989544, Length=252, Percent_Identity=32.5396825396825, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI281363755, Length=219, Percent_Identity=36.986301369863, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281363751, Length=219, Percent_Identity=36.986301369863, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24655690, Length=219, Percent_Identity=36.986301369863, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24655685, Length=219, Percent_Identity=36.986301369863, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI281363761, Length=213, Percent_Identity=37.5586854460094, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363759, Length=213, Percent_Identity=37.5586854460094, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363753, Length=213, Percent_Identity=37.5586854460094, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363763, Length=219, Percent_Identity=36.986301369863, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363757, Length=219, Percent_Identity=36.986301369863, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17136738, Length=219, Percent_Identity=36.986301369863, Blast_Score=141, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28198; Mature: 28066

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIQLVDSIRDRVSDAEWQMRVDLAAAYRLVAHFGWDDLIFTHISARVPDAPDQFLINP
CCEEHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECH
YGMMFDEITASSLVKVDHEGQPVLETPYDVNPAGFIIHSAVHEARPEVGCVMHTHTSHGV
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCE
AVSAQQAGLLPISQQSMFALTGLAYHDYEGVALREDEKARLVADLGRCKQMILRNHGLLT
EEECCCCCCCCCCCCHHHHHHCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEE
CGRTVSDAFLTMYTLESACRIQILAQSGGTPLTMVPEAASANMGQQARQATKGKGSNLAW
ECCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHCCCCCHHHHHHHCCCCCCCCH
PGLLRRLDRINPDYRN
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQIQLVDSIRDRVSDAEWQMRVDLAAAYRLVAHFGWDDLIFTHISARVPDAPDQFLINP
CEEHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECH
YGMMFDEITASSLVKVDHEGQPVLETPYDVNPAGFIIHSAVHEARPEVGCVMHTHTSHGV
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCE
AVSAQQAGLLPISQQSMFALTGLAYHDYEGVALREDEKARLVADLGRCKQMILRNHGLLT
EEECCCCCCCCCCCCHHHHHHCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEE
CGRTVSDAFLTMYTLESACRIQILAQSGGTPLTMVPEAASANMGQQARQATKGKGSNLAW
ECCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHCCCCCHHHHHHHCCCCCCCCH
PGLLRRLDRINPDYRN
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]