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The map label for this gene is phnR [H]

Identifier: 116694783

GI number: 116694783

Start: 949779

End: 950675

Strand: Direct

Name: phnR [H]

Synonym: H16_B0837

Alternate gene names: 116694783

Gene position: 949779-950675 (Clockwise)

Preceding gene: 116694780

Following gene: 116694786

Centisome position: 32.61

GC content: 66.44

Gene sequence:

>897_bases
ATGCCGCACAAGCTGCCTCCGCTCAACGCCCTGCGCATCTTCGAGGTTGCCGCCCGCGCCGGCAGCTATTCGGCGGCCGC
GCGCGAGCTGCACCTGACCCATGGCGCGGTCAGCCGGCAGATCGAGATCCTGGAGCAATGGCTGGGGCAGCCGCTGTTTA
TCCGCCAGGGCCAGCGCATGGTGCCGACTGTCCACGCGCAGGCGTTCGCGCGCGAGATCAGCACGGCGTTCGACCAGATC
AGCGCCGCTTCCGAGCGCTATGGCCGCATCGCCACGCGCAAGGTGGTGCGCGTCAATGCCCCGGCAACCTTTGCCATGCG
CTGGCTGATTCCGCGGCTGGATGACTTCCGGCACCGGCAGCCCGAGGTCGACGTGCGTGTGTCGACCGCCTTCAGCAACG
AGGCAGGATTCAATGGCACCTTTGACGTGGCGATACGGCGCACCCTCGAACGTGGCGAACAGTTCGAGTCAGTGCCGATC
TTTTCGGAGTACCAGACGGTGATCGCCAGTCCGGCGATGCTGGAGCAGGTGCCGTTGCAAGGTGTAGAGGACCTGGTGGA
GGGCGTCTTCCTCTATACGGAGACCCGGCCGGGAAGCTGGGAATCGTGGCTGCAGCAGGCGGGACATGCCTCGCTGCTGC
CGGTGCGAACGCTGCGCTTCGACCATTTCTTTGTCACGCTGCAGGCGGTGGTGGACAGCCTTGGCTTTGCCATCGGCACC
TTTCCGACCCTGGAAACGGACCGCGCGGACGGGCGCATTGCGACGCCGTTTGCCGCCATCCGCGCACCGGGCAATACCTA
TCATGCGCTGGTGCCGCGCGATGCCGACAAGCCGCTGCATCTGCGCGCGTTCCTGGAGTGGCTGCAGCAGCAGGGGCAGA
CGGCCGCAGCGACCTGA

Upstream 100 bases:

>100_bases
GGAAGGATTGTGAAGGGGATTTGCCTGAGACCCATGCTAAGTTAGGCGATAAACTCTCACAATCGAGTTTATTTTCCAGA
TTCTGTCAGGAAATCTCACA

Downstream 100 bases:

>100_bases
AGCGGCGCGAGCCGGGGAAGCGGGGCGAGTGTGGCGCGCGGCCCGCGCGATGCGGGCCTGCGCTGAGACGGGAGCTCAGG
CCCCGTCGAGGAACGCGCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPHKLPPLNALRIFEVAARAGSYSAAARELHLTHGAVSRQIEILEQWLGQPLFIRQGQRMVPTVHAQAFAREISTAFDQI
SAASERYGRIATRKVVRVNAPATFAMRWLIPRLDDFRHRQPEVDVRVSTAFSNEAGFNGTFDVAIRRTLERGEQFESVPI
FSEYQTVIASPAMLEQVPLQGVEDLVEGVFLYTETRPGSWESWLQQAGHASLLPVRTLRFDHFFVTLQAVVDSLGFAIGT
FPTLETDRADGRIATPFAAIRAPGNTYHALVPRDADKPLHLRAFLEWLQQQGQTAAAT

Sequences:

>Translated_298_residues
MPHKLPPLNALRIFEVAARAGSYSAAARELHLTHGAVSRQIEILEQWLGQPLFIRQGQRMVPTVHAQAFAREISTAFDQI
SAASERYGRIATRKVVRVNAPATFAMRWLIPRLDDFRHRQPEVDVRVSTAFSNEAGFNGTFDVAIRRTLERGEQFESVPI
FSEYQTVIASPAMLEQVPLQGVEDLVEGVFLYTETRPGSWESWLQQAGHASLLPVRTLRFDHFFVTLQAVVDSLGFAIGT
FPTLETDRADGRIATPFAAIRAPGNTYHALVPRDADKPLHLRAFLEWLQQQGQTAAAT
>Mature_297_residues
PHKLPPLNALRIFEVAARAGSYSAAARELHLTHGAVSRQIEILEQWLGQPLFIRQGQRMVPTVHAQAFAREISTAFDQIS
AASERYGRIATRKVVRVNAPATFAMRWLIPRLDDFRHRQPEVDVRVSTAFSNEAGFNGTFDVAIRRTLERGEQFESVPIF
SEYQTVIASPAMLEQVPLQGVEDLVEGVFLYTETRPGSWESWLQQAGHASLLPVRTLRFDHFFVTLQAVVDSLGFAIGTF
PTLETDRADGRIATPFAAIRAPGNTYHALVPRDADKPLHLRAFLEWLQQQGQTAAAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=30.6896551724138, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=29.0836653386454, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=24.2424242424242, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.4295532646048, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33329; Mature: 33198

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHKLPPLNALRIFEVAARAGSYSAAARELHLTHGAVSRQIEILEQWLGQPLFIRQGQRM
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
VPTVHAQAFAREISTAFDQISAASERYGRIATRKVVRVNAPATFAMRWLIPRLDDFRHRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCC
PEVDVRVSTAFSNEAGFNGTFDVAIRRTLERGEQFESVPIFSEYQTVIASPAMLEQVPLQ
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHCHHHHHHCCCC
GVEDLVEGVFLYTETRPGSWESWLQQAGHASLLPVRTLRFDHFFVTLQAVVDSLGFAIGT
HHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FPTLETDRADGRIATPFAAIRAPGNTYHALVPRDADKPLHLRAFLEWLQQQGQTAAAT
CCCCCCCCCCCEEECCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PHKLPPLNALRIFEVAARAGSYSAAARELHLTHGAVSRQIEILEQWLGQPLFIRQGQRM
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
VPTVHAQAFAREISTAFDQISAASERYGRIATRKVVRVNAPATFAMRWLIPRLDDFRHRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCC
PEVDVRVSTAFSNEAGFNGTFDVAIRRTLERGEQFESVPIFSEYQTVIASPAMLEQVPLQ
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHCHHHHHHCCCC
GVEDLVEGVFLYTETRPGSWESWLQQAGHASLLPVRTLRFDHFFVTLQAVVDSLGFAIGT
HHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FPTLETDRADGRIATPFAAIRAPGNTYHALVPRDADKPLHLRAFLEWLQQQGQTAAAT
CCCCCCCCCCCEEECCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]