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The map label for this gene is ilvB [H]

Identifier: 116694686

GI number: 116694686

Start: 828099

End: 829814

Strand: Direct

Name: ilvB [H]

Synonym: H16_B0735

Alternate gene names: 116694686

Gene position: 828099-829814 (Clockwise)

Preceding gene: 116694685

Following gene: 116694687

Centisome position: 28.43

GC content: 68.71

Gene sequence:

>1716_bases
ATGCCTAATCAACAACAGTACGAGCAACAGCAGGTCACCGTCGGTTGCGCCATCACGGCGTTTCTCGAGCAGTGCGGCGT
CAAGGCCGCCTTCGGCGTGATCTCGATCCACAATATGCCGATCCTCGACGCCATGGGCGAGCGCGGCAAGATCCGCTTCG
TGCCGGCGCGCGGCGAAGCGGGCGGCACCAATATGGCCGATGCCTATGCCCGCACCACCGGTGGCCTGGGCGTGTGCCTG
ACCAGCACCGGCACCGCCGCGGGCAACGCCGCCGGCGCCATGGTCGAAGCGCTGACCGCCGGCACGCCGATGCTGCATAT
CACCGGCCAGATCGAAACGCCTTACCTGGACCAGAGCCTCGCCTACATCCACGAAGCGCCGGACCAGCTCACCATGCTCA
AGGCCGTGTCCAAGGCCGCCTTCCGCATCCGCAGCGCCGACACCGCCATCAGCACCATCAAGCTGGCCGTGCAGACCGCA
CTGACGGCGCCGACCGGCCCGGTCAGCGTGGAGATCCCCATCGACATCCAGGCCGCGCTGATCGAGATGCCGGCCGACCT
GCAGCCGCTGCCGGTGCCGCAGCACGTGCCGTCGGCACATGCGCTGGACGCGCTGGCCGAGCGCCTGGCCAAGGCCAAGC
GCCCGCTGCTGTGGCTGGGCGGCGGTGCGCGCCACGCCAGCAAGCAGGTGCAGCGCCTGCTGGACCTGGGCTTCGGCGTG
GTCACCAGCACGCAAGGCCGCGGCATCGTGCCGGAAGACGATCCCCGTTCGCTGGGCGCCTACAACCTGCATAAGCCGGT
CGAGGCCTTCTACCAGACCTGCGACGCGATGCTGGTGGTGGGCTCGCGCCTGCGCGGCAATGAAACCCTGAAGTACGAAC
TGAAGCTGCCGCGCCCGCTGCTGCGCATCGACGCCGACCCGGCCGCCGAAGGCCGCTGCTACCTGAGCGACTACTTTGTC
AGCGGCGACGCCGCGCTGGCGCTGGACGCCCTGGCGGATCGGCTTGAAAAGCGCATGCAGATCGACCCGGCCTTCGCCGC
CGACCTGCGCCGCGCCCATGACACCGCCGTCAACAGCCTGGTCGACGGCCTGGGCCCGTACTCGGCGCTGGTGCAGGCGC
TGCAGGGCGCGGTCGGCCGCAGCTTCAACTGGGTGCGCGACGTGACCGTGTCGAACAGCACCTGGGGCAACCGCCATCTG
CGCATCTTCGATTCGCGTGCCGGCGTGCATGCGCTGGGCGGCGGCATCGGCCAGGGCCTGGCGATGGGCATCGGCGCCGC
GATCGGTGCTGCGGCGACCGCTTCGGGCAAGAAGACCTTCACGCTGGCCGGCGACGGCGGTTTTATCCTGAACCTGGGCG
AACTGGCCACCGCGGTGCAGGAACGCGCCGACATGGTGATCGTGCTGATGAACGACAAGGGCTACGGCGTGATCAAGAAC
ATCCAGGACGCCCAGTACGGCGGCCGCCGCCACTACGTGGACCTGCATACCCCGGACTACGCCACGCTGGCCAAGTCGCT
GTCGCTGCGCCATGCGCGCGTGAGCAACCTGGCCGAGGTCGGCGCCGCGCTGGAAGCGGCCACGGTCGAGTCGGGCCCGT
TCCTGCTGGAGATCGACATGCTGTCGATCGGTTCCTTCAAGACCATCTTCGCCGGCCCCCCGGTGAACAAGGACAACGAA
GAGCCGGCGCGCGAACAAACCACTGTCACCGCCTGA

Upstream 100 bases:

>100_bases
CTGCCGGTCGAGGCAGCCCGCGCCATCCTGTTCCTTGCCTCGCCGTTGTCTTCGTACACCACGGGCAGCCATATCGATAT
CTCCGGAGGACACTCCCGTC

Downstream 100 bases:

>100_bases
GGGAGACCGGAGATGCTGCATGTGTCCATGGTTGGCTGCGGCGCGATCGGCCGCGGCGTGATGGAATTGCTCAAGAGCGA
TCCCGAGGTGGTGTTCGACG

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGKIRFVPARGEAGGTNMADAYARTTGGLGVCL
TSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRIRSADTAISTIKLAVQTA
LTAPTGPVSVEIPIDIQAALIEMPADLQPLPVPQHVPSAHALDALAERLAKAKRPLLWLGGGARHASKQVQRLLDLGFGV
VTSTQGRGIVPEDDPRSLGAYNLHKPVEAFYQTCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPAAEGRCYLSDYFV
SGDAALALDALADRLEKRMQIDPAFAADLRRAHDTAVNSLVDGLGPYSALVQALQGAVGRSFNWVRDVTVSNSTWGNRHL
RIFDSRAGVHALGGGIGQGLAMGIGAAIGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
IQDAQYGGRRHYVDLHTPDYATLAKSLSLRHARVSNLAEVGAALEAATVESGPFLLEIDMLSIGSFKTIFAGPPVNKDNE
EPAREQTTVTA

Sequences:

>Translated_571_residues
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGKIRFVPARGEAGGTNMADAYARTTGGLGVCL
TSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRIRSADTAISTIKLAVQTA
LTAPTGPVSVEIPIDIQAALIEMPADLQPLPVPQHVPSAHALDALAERLAKAKRPLLWLGGGARHASKQVQRLLDLGFGV
VTSTQGRGIVPEDDPRSLGAYNLHKPVEAFYQTCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPAAEGRCYLSDYFV
SGDAALALDALADRLEKRMQIDPAFAADLRRAHDTAVNSLVDGLGPYSALVQALQGAVGRSFNWVRDVTVSNSTWGNRHL
RIFDSRAGVHALGGGIGQGLAMGIGAAIGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
IQDAQYGGRRHYVDLHTPDYATLAKSLSLRHARVSNLAEVGAALEAATVESGPFLLEIDMLSIGSFKTIFAGPPVNKDNE
EPAREQTTVTA
>Mature_570_residues
PNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGKIRFVPARGEAGGTNMADAYARTTGGLGVCLT
STGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRIRSADTAISTIKLAVQTAL
TAPTGPVSVEIPIDIQAALIEMPADLQPLPVPQHVPSAHALDALAERLAKAKRPLLWLGGGARHASKQVQRLLDLGFGVV
TSTQGRGIVPEDDPRSLGAYNLHKPVEAFYQTCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPAAEGRCYLSDYFVS
GDAALALDALADRLEKRMQIDPAFAADLRRAHDTAVNSLVDGLGPYSALVQALQGAVGRSFNWVRDVTVSNSTWGNRHLR
IFDSRAGVHALGGGIGQGLAMGIGAAIGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKNI
QDAQYGGRRHYVDLHTPDYATLAKSLSLRHARVSNLAEVGAALEAATVESGPFLLEIDMLSIGSFKTIFAGPPVNKDNEE
PAREQTTVTA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=564, Percent_Identity=23.4042553191489, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI93004078, Length=497, Percent_Identity=23.943661971831, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=26.2081784386617, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=27.4436090225564, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=535, Percent_Identity=24.6728971962617, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787096, Length=537, Percent_Identity=23.6499068901304, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788716, Length=493, Percent_Identity=24.5436105476673, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17531299, Length=546, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531301, Length=546, Percent_Identity=24.3589743589744, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=24.9566724436742, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6321524, Length=554, Percent_Identity=23.2851985559567, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=539, Percent_Identity=22.4489795918367, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60377; Mature: 60245

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGKIRFVPARGEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCC
GGTNMADAYARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSL
CCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AYIHEAPDQLTMLKAVSKAAFRIRSADTAISTIKLAVQTALTAPTGPVSVEIPIDIQAAL
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
IEMPADLQPLPVPQHVPSAHALDALAERLAKAKRPLLWLGGGARHASKQVQRLLDLGFGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCE
VTSTQGRGIVPEDDPRSLGAYNLHKPVEAFYQTCDAMLVVGSRLRGNETLKYELKLPRPL
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
LRIDADPAAEGRCYLSDYFVSGDAALALDALADRLEKRMQIDPAFAADLRRAHDTAVNSL
EEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VDGLGPYSALVQALQGAVGRSFNWVRDVTVSNSTWGNRHLRIFDSRAGVHALGGGIGQGL
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHHHH
AMGIGAAIGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCEEECC
IQDAQYGGRRHYVDLHTPDYATLAKSLSLRHARVSNLAEVGAALEAATVESGPFLLEIDM
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEE
LSIGSFKTIFAGPPVNKDNEEPAREQTTVTA
ECCCCEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGKIRFVPARGEA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCC
GGTNMADAYARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSL
CCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AYIHEAPDQLTMLKAVSKAAFRIRSADTAISTIKLAVQTALTAPTGPVSVEIPIDIQAAL
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
IEMPADLQPLPVPQHVPSAHALDALAERLAKAKRPLLWLGGGARHASKQVQRLLDLGFGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCE
VTSTQGRGIVPEDDPRSLGAYNLHKPVEAFYQTCDAMLVVGSRLRGNETLKYELKLPRPL
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
LRIDADPAAEGRCYLSDYFVSGDAALALDALADRLEKRMQIDPAFAADLRRAHDTAVNSL
EEECCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VDGLGPYSALVQALQGAVGRSFNWVRDVTVSNSTWGNRHLRIFDSRAGVHALGGGIGQGL
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHHHH
AMGIGAAIGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCEEECC
IQDAQYGGRRHYVDLHTPDYATLAKSLSLRHARVSNLAEVGAALEAATVESGPFLLEIDM
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEE
LSIGSFKTIFAGPPVNKDNEEPAREQTTVTA
ECCCCEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]