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The map label for this gene is yagI [H]

Identifier: 116694684

GI number: 116694684

Start: 826404

End: 827297

Strand: Direct

Name: yagI [H]

Synonym: H16_B0733

Alternate gene names: 116694684

Gene position: 826404-827297 (Clockwise)

Preceding gene: 116694683

Following gene: 116694685

Centisome position: 28.37

GC content: 69.02

Gene sequence:

>894_bases
ATGCCGGCGAACGTCCGTCAGCACGCATCCACCGAAGCCATGAGCAGCAACCCCATCCCGGCCACGTCCGCCGCCAGCGA
AACCCCGGACAAGTACATCGTCCCGGGCCTGGAGCGCGGCCTGCGCCTGCTGAGCGAGTTCAGCAGCAAGGACCGCACCA
TGTCGGCAGCGGAACTGGCGCGCCGCCTGAAGGTGCCGCGCTCGACCGTGTTCCGCCTGCTGGCCACGCTGGAAATGCTG
GGCTTTGTCGAGCGCGCCGATGGCGGCCGGGATTTCCGGCTGGGCATGGCCGTGCTGCGCCTGGGGTTTGAATACCTGGC
GTCGCTGGAGCTGACCGAGCTGGGCCGCCCGCTGCTGGAGCGGCTGCGCGACGAGATCGGCTATCCCAGCAACCTGGTGG
TGCGCGACGGGCGTTCGATCGTCTACGTGGCCAAGGCGGTGTCGCCGCGGCCGTTTGCCAGCTCGGTCAACGTCGGCACG
CGCCTGCCCGCGCATGCCACGGTGCTGGGGCGCGTGCTGCTGGAAGACCTGTCGCTGGCGGAGCTGCGCACGCTGTACCC
GGAGACGCAGCTTGCGGTGTTTTCCGAGAGCACGCCGCGCACCGTCGACGAGCTGCACGAGATCGTGCAGCGCGACCGCG
AGCGCGGCTACGTGCTGCAGGAAGGCTTTTTCGAAGCCAGCATTTCCACCATCGCCGCGCCCGTGCGCGACCGCACCGGC
AAGGTGGTGGCGGCGTTGGGCGCCACCATCCCCGCCGCGCATATCGATCCGGCGCAACTGGATGCGATGGTGGAGAAGGT
CAGGGATACCGCGGGCGAGTTGTCGCGCCTGCTCGACTACCGCCCGTCGCTGCGCAGCAGCGGCGCCAAGGTGGTGAATC
TGTATCGGGAGTGA

Upstream 100 bases:

>100_bases
TCGGCGAGGAAGACGCGCTGATGTGCGTGATGCTGGGCGCGAAGAAGCCGATCACGCCGACCTACCCGCCCGAGCATCCG
CTGACGAAGATCAAGCGCTG

Downstream 100 bases:

>100_bases
GGACAATGTCCATGATTGAACTCGGCGGCAAGGTTGCCGTCGTCACCGGCGGGTCCTCCGGCATCGGGCTGGCGACCGTC
GAGCTGCTGCTCGAAGCGGG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPANVRQHASTEAMSSNPIPATSAASETPDKYIVPGLERGLRLLSEFSSKDRTMSAAELARRLKVPRSTVFRLLATLEML
GFVERADGGRDFRLGMAVLRLGFEYLASLELTELGRPLLERLRDEIGYPSNLVVRDGRSIVYVAKAVSPRPFASSVNVGT
RLPAHATVLGRVLLEDLSLAELRTLYPETQLAVFSESTPRTVDELHEIVQRDRERGYVLQEGFFEASISTIAAPVRDRTG
KVVAALGATIPAAHIDPAQLDAMVEKVRDTAGELSRLLDYRPSLRSSGAKVVNLYRE

Sequences:

>Translated_297_residues
MPANVRQHASTEAMSSNPIPATSAASETPDKYIVPGLERGLRLLSEFSSKDRTMSAAELARRLKVPRSTVFRLLATLEML
GFVERADGGRDFRLGMAVLRLGFEYLASLELTELGRPLLERLRDEIGYPSNLVVRDGRSIVYVAKAVSPRPFASSVNVGT
RLPAHATVLGRVLLEDLSLAELRTLYPETQLAVFSESTPRTVDELHEIVQRDRERGYVLQEGFFEASISTIAAPVRDRTG
KVVAALGATIPAAHIDPAQLDAMVEKVRDTAGELSRLLDYRPSLRSSGAKVVNLYRE
>Mature_296_residues
PANVRQHASTEAMSSNPIPATSAASETPDKYIVPGLERGLRLLSEFSSKDRTMSAAELARRLKVPRSTVFRLLATLEMLG
FVERADGGRDFRLGMAVLRLGFEYLASLELTELGRPLLERLRDEIGYPSNLVVRDGRSIVYVAKAVSPRPFASSVNVGTR
LPAHATVLGRVLLEDLSLAELRTLYPETQLAVFSESTPRTVDELHEIVQRDRERGYVLQEGFFEASISTIAAPVRDRTGK
VVAALGATIPAAHIDPAQLDAMVEKVRDTAGELSRLLDYRPSLRSSGAKVVNLYRE

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786715, Length=245, Percent_Identity=27.3469387755102, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082359, Length=258, Percent_Identity=26.7441860465116, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=27.0916334661355, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788131, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789999, Length=252, Percent_Identity=24.2063492063492, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32658; Mature: 32527

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANVRQHASTEAMSSNPIPATSAASETPDKYIVPGLERGLRLLSEFSSKDRTMSAAELA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RRLKVPRSTVFRLLATLEMLGFVERADGGRDFRLGMAVLRLGFEYLASLELTELGRPLLE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLRDEIGYPSNLVVRDGRSIVYVAKAVSPRPFASSVNVGTRLPAHATVLGRVLLEDLSLA
HHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELRTLYPETQLAVFSESTPRTVDELHEIVQRDRERGYVLQEGFFEASISTIAAPVRDRTG
HHHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KVVAALGATIPAAHIDPAQLDAMVEKVRDTAGELSRLLDYRPSLRSSGAKVVNLYRE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCC
>Mature Secondary Structure 
PANVRQHASTEAMSSNPIPATSAASETPDKYIVPGLERGLRLLSEFSSKDRTMSAAELA
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RRLKVPRSTVFRLLATLEMLGFVERADGGRDFRLGMAVLRLGFEYLASLELTELGRPLLE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLRDEIGYPSNLVVRDGRSIVYVAKAVSPRPFASSVNVGTRLPAHATVLGRVLLEDLSLA
HHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELRTLYPETQLAVFSESTPRTVDELHEIVQRDRERGYVLQEGFFEASISTIAAPVRDRTG
HHHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KVVAALGATIPAAHIDPAQLDAMVEKVRDTAGELSRLLDYRPSLRSSGAKVVNLYRE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]