BacMap

Definition	Ralstonia eutropha H16 chromosome 2, complete sequence.
Accession	NC_008314
Length	2,912,490

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The map label for this gene is paaF [C]

Identifier: 116694335

GI number: 116694335

Start: 433980

End: 434750

Strand: Direct

Name: paaF [C]

Synonym: H16_B0382

Alternate gene names: 116694335

Gene position: 433980-434750 (Clockwise)

Preceding gene: 116694334

Following gene: 116694336

Centisome position: 14.9

GC content: 70.17

Gene sequence:

>771_bases
ATGAGCGTTCTGCATCGCATCGAGAACGGCGTCGGCTGGCTGACCATGGACCGCCCGGCGGCCATGAACAGCCTGAACCG
CGAGATGGCCGCCGCCATGACCGCGCAACTCAGGGCATGGCGCGACGACCCCGCCGTGCGCGCGGTGGTGCTTACCGGCA
ACGGCCGTGCGTTCTGCGCCGGGGCCGACCTCAAGCAAGTGGCCGAGCCGCCTGTGACCGGCGAGCCGGATTTCCTCGAC
GTGGTGGTCGGCTTCGTCGACACGCTGCGCGCGTTTCCCAGGCCCGTCATCGCCGCCGTGAATGGCATGGCGCTCGCGGG
CGGGCTGGAAACGGTGATGGCCTGCGACCTCGTCCTCGCCGCCGAGAGCGCGAAGCTGGGCGACGCGCACTCGAACTTCG
GCGTGTTCCCCGGGGCGGGCGGCGCGGCGGTGCTGCCGCGCAAGGTGCCTGTCAACGTGGCGCGCTGGCTGCTGTTCACC
GGCGCAAGCTTCACCGCCGCGCAGATGGAACGCTTCGGCCTGGTCAACGAAGTTGTGCCGGACGCCGAACTCGCTGCCCG
CGCACAGGCCGTGGGCGAATTGCTGGCGGCCAGGAGCCCGCTGGTACTCGCCCGCATGAAGCGCGTGGTGGCCGAGGCCG
CCGACAAGCCGGTTGCCGACGCGCTCCGTCAGGAACTGCTCGAACTGCGCAACCACCAGCGCTCCCATGACATGCGCGAG
GGCTTGCTGGCCTTCGCCGAAAAGCGGCAGCCCGTCTTCAAGGGCTGCTGA

Upstream 100 bases:

>100_bases
GCGCTGCAGCAGCGCGGCCTGAAGCGCGGGCTCGCCAGCCTGTGCCTGGGCGGTGGCGAAGCCACGGCCATCGGCCTCGA
ACTGTGCTGAGGAGCCGGCC

Downstream 100 bases:

>100_bases
ACCGACACAAGGAACCACCATGGACTTGCTCTACTCGGAGGAGCACAAGGCGTTCCGGCAGGAAGTGCGCGACTTTCTCG
CCGCCAACCTGCCGGACGAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSVLHRIENGVGWLTMDRPAAMNSLNREMAAAMTAQLRAWRDDPAVRAVVLTGNGRAFCAGADLKQVAEPPVTGEPDFLD
VVVGFVDTLRAFPRPVIAAVNGMALAGGLETVMACDLVLAAESAKLGDAHSNFGVFPGAGGAAVLPRKVPVNVARWLLFT
GASFTAAQMERFGLVNEVVPDAELAARAQAVGELLAARSPLVLARMKRVVAEAADKPVADALRQELLELRNHQRSHDMRE
GLLAFAEKRQPVFKGC

Sequences:

>Translated_256_residues
MSVLHRIENGVGWLTMDRPAAMNSLNREMAAAMTAQLRAWRDDPAVRAVVLTGNGRAFCAGADLKQVAEPPVTGEPDFLD
VVVGFVDTLRAFPRPVIAAVNGMALAGGLETVMACDLVLAAESAKLGDAHSNFGVFPGAGGAAVLPRKVPVNVARWLLFT
GASFTAAQMERFGLVNEVVPDAELAARAQAVGELLAARSPLVLARMKRVVAEAADKPVADALRQELLELRNHQRSHDMRE
GLLAFAEKRQPVFKGC
>Mature_255_residues
SVLHRIENGVGWLTMDRPAAMNSLNREMAAAMTAQLRAWRDDPAVRAVVLTGNGRAFCAGADLKQVAEPPVTGEPDFLDV
VVGFVDTLRAFPRPVIAAVNGMALAGGLETVMACDLVLAAESAKLGDAHSNFGVFPGAGGAAVLPRKVPVNVARWLLFTG
ASFTAAQMERFGLVNEVVPDAELAARAQAVGELLAARSPLVLARMKRVVAEAADKPVADALRQELLELRNHQRSHDMREG
LLAFAEKRQPVFKGC

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=33.739837398374, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=27.2357723577236, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=26.2886597938144, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=26.2886597938144, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=29.4444444444444, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=25, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=25, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=199, Percent_Identity=30.1507537688442, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=28.9099526066351, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=28.9099526066351, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI68989263, Length=180, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=25.2, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=25.8215962441315, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=25.8215962441315, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757968, Length=256, Percent_Identity=24.609375, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=256, Percent_Identity=24.609375, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=26.2008733624454, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=26.2008733624454, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=214, Percent_Identity=26.6355140186916, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=214, Percent_Identity=26.6355140186916, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=36.1111111111111, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=36.3984674329502, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=36.9649805447471, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=31.7460317460317, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=30.3664921465969, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788682, Length=184, Percent_Identity=32.6086956521739, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=28.4403669724771, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=36.3265306122449, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=34.1463414634146, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=32.4324324324324, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17549921, Length=235, Percent_Identity=28.0851063829787, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=252, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=192, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=24.6031746031746, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=22.7799227799228, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=31.3186813186813, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=181, Percent_Identity=28.1767955801105, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=194, Percent_Identity=29.8969072164948, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=194, Percent_Identity=29.8969072164948, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=205, Percent_Identity=26.3414634146341, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=34.2857142857143, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=34.2857142857143, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=35.7142857142857, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=27.1317829457364, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24650670, Length=179, Percent_Identity=30.1675977653631, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=25.1937984496124, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27287; Mature: 27156

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLHRIENGVGWLTMDRPAAMNSLNREMAAAMTAQLRAWRDDPAVRAVVLTGNGRAFCA
CHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEE
GADLKQVAEPPVTGEPDFLDVVVGFVDTLRAFPRPVIAAVNGMALAGGLETVMACDLVLA
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
AESAKLGDAHSNFGVFPGAGGAAVLPRKVPVNVARWLLFTGASFTAAQMERFGLVNEVVP
HCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
DAELAARAQAVGELLAARSPLVLARMKRVVAEAADKPVADALRQELLELRNHQRSHDMRE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLLAFAEKRQPVFKGC
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVLHRIENGVGWLTMDRPAAMNSLNREMAAAMTAQLRAWRDDPAVRAVVLTGNGRAFCA
HHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEE
GADLKQVAEPPVTGEPDFLDVVVGFVDTLRAFPRPVIAAVNGMALAGGLETVMACDLVLA
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
AESAKLGDAHSNFGVFPGAGGAAVLPRKVPVNVARWLLFTGASFTAAQMERFGLVNEVVP
HCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
DAELAARAQAVGELLAARSPLVLARMKRVVAEAADKPVADALRQELLELRNHQRSHDMRE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLLAFAEKRQPVFKGC
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA