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The map label for this gene is pimD [H]

Identifier: 116694326

GI number: 116694326

Start: 420617

End: 421753

Strand: Direct

Name: pimD [H]

Synonym: H16_B0372

Alternate gene names: 116694326

Gene position: 420617-421753 (Clockwise)

Preceding gene: 116694325

Following gene: 116694327

Centisome position: 14.44

GC content: 70.71

Gene sequence:

>1137_bases
ATGAACTTCAATCTGAACGACGAGCAGCGCCAGCTTGCCGACAGCCTGTCACGCCTGCTGGCCGATCACTACAGCTTCGA
GAAGCGCCGTGCGTTGACAAGGCCGGAAGCCACCTGCGAGCCCGCAATCTGGACGCACCTTGCCGCGCTTGGCGTGACGG
CGCTAGGCATCCCCGAGGCATTCGGCGGCCTGGGCGGCGGCACACAGGACCGCCTGCCGGTCATGCAGGCCTTCGGGCGG
GCGCTGGCCATCGAGCCCTACCTCGCCTCGGCCGTGCTCGGCACGACGGCTGTGCGCGAGGGCGGCAGCAGCGCGCAGCA
GGCGGAACTCCTGCCTTCGCTGGCAAGCGGGGAAATGCGTATCGCGTGGGCCCATGACGAGGCGGCCGGCCGGCACGCGC
CGCTGTGGGTCGAGACGACCGCCAGGTCCGGTCCCGATGGCTGGCGCCTGGACGGCGCCAAGGCCCTGGTGCTGCACGGT
GGCGCCGTCTCGCGCATCGTCGTGACCGCGCGCACGCGCGGCAAGGCGCAGGACGAGGATGGCCTTGCGCTATTCCTTGT
CGATCCGCAGGCGGACGGCGTGACGCGGCGCGCCTACCGGCTGGTTGACGACACGCCAGCCGTCGAACTGACGCTGCGCA
ACGCGCAGGCCTGCCCGCTGGGCGATGTCTCCGACAGCGTCCGCGCGACCGCGGTGATCCGCGCCACGCAGGCCGCCGGC
ATCGCAGCGGCCTGCGCCGACATGGTCGGCACCATGGAGACGGCGCTTCAGCTCACCACCGACTACGTTCGCACGCGCAA
GCAGTTCGGCCGGCCGATCGGCGACTACCAGTCGATGCGCCACCGCATCGCGGACATGTGCGTGAGCCTCGAACTCGCGC
GCAGCATGGCGGTGGCCGCCGCCGTTGCGGGCGATGATCCGTCCGGGGACGATGGGCTGGCCGAACTGTCGCGTGCCAAG
CTCGTCATTGGCCGCCATGCCCGCACGCTCTGCCACGCGGCGATCCAGTCGCACGGCGGCATCGGCCTGACCGAAGAGTA
CGCCGTGGGGCATTGCCTGCGCCGCGTGCACGTGCTGGACCAGTTGTTCGGGGACTGCGATGCGCATACCCGGTGGCTGG
CCGGACAACTGGGCTGA

Upstream 100 bases:

>100_bases
CGCCGTGTGGGGATACTTCTTCCATCGGGCCTCGACGATCTTCGGCGGTTCCAGCGAAGTCCAGAAGGACATTCTTGCCA
AGGCAACCCGGGGTTAAATC

Downstream 100 bases:

>100_bases
GCGCTGCGCGATCGCGTGATTTCCGGTCAGGGCCCCGGCGCAGTCACGGCCGGGGCTGTCCCTTTGCTCGCCCAGGCTCG
ACATGCGTAGCGCTCCCCCG

Product: pimeloyl-CoA dehydrogenase (small subunit)

Products: 6-carboxyhex-2-enoyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNFNLNDEQRQLADSLSRLLADHYSFEKRRALTRPEATCEPAIWTHLAALGVTALGIPEAFGGLGGGTQDRLPVMQAFGR
ALAIEPYLASAVLGTTAVREGGSSAQQAELLPSLASGEMRIAWAHDEAAGRHAPLWVETTARSGPDGWRLDGAKALVLHG
GAVSRIVVTARTRGKAQDEDGLALFLVDPQADGVTRRAYRLVDDTPAVELTLRNAQACPLGDVSDSVRATAVIRATQAAG
IAAACADMVGTMETALQLTTDYVRTRKQFGRPIGDYQSMRHRIADMCVSLELARSMAVAAAVAGDDPSGDDGLAELSRAK
LVIGRHARTLCHAAIQSHGGIGLTEEYAVGHCLRRVHVLDQLFGDCDAHTRWLAGQLG

Sequences:

>Translated_378_residues
MNFNLNDEQRQLADSLSRLLADHYSFEKRRALTRPEATCEPAIWTHLAALGVTALGIPEAFGGLGGGTQDRLPVMQAFGR
ALAIEPYLASAVLGTTAVREGGSSAQQAELLPSLASGEMRIAWAHDEAAGRHAPLWVETTARSGPDGWRLDGAKALVLHG
GAVSRIVVTARTRGKAQDEDGLALFLVDPQADGVTRRAYRLVDDTPAVELTLRNAQACPLGDVSDSVRATAVIRATQAAG
IAAACADMVGTMETALQLTTDYVRTRKQFGRPIGDYQSMRHRIADMCVSLELARSMAVAAAVAGDDPSGDDGLAELSRAK
LVIGRHARTLCHAAIQSHGGIGLTEEYAVGHCLRRVHVLDQLFGDCDAHTRWLAGQLG
>Mature_378_residues
MNFNLNDEQRQLADSLSRLLADHYSFEKRRALTRPEATCEPAIWTHLAALGVTALGIPEAFGGLGGGTQDRLPVMQAFGR
ALAIEPYLASAVLGTTAVREGGSSAQQAELLPSLASGEMRIAWAHDEAAGRHAPLWVETTARSGPDGWRLDGAKALVLHG
GAVSRIVVTARTRGKAQDEDGLALFLVDPQADGVTRRAYRLVDDTPAVELTLRNAQACPLGDVSDSVRATAVIRATQAAG
IAAACADMVGTMETALQLTTDYVRTRKQFGRPIGDYQSMRHRIADMCVSLELARSMAVAAAVAGDDPSGDDGLAELSRAK
LVIGRHARTLCHAAIQSHGGIGLTEEYAVGHCLRRVHVLDQLFGDCDAHTRWLAGQLG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=364, Percent_Identity=25, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI21361497, Length=345, Percent_Identity=24.9275362318841, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI76496475, Length=390, Percent_Identity=25.3846153846154, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4557235, Length=390, Percent_Identity=25.3846153846154, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI7656849, Length=364, Percent_Identity=24.1758241758242, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4557233, Length=337, Percent_Identity=24.9258160237389, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4501859, Length=332, Percent_Identity=22.8915662650602, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI187960098, Length=331, Percent_Identity=25.3776435045317, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4557231, Length=331, Percent_Identity=25.3776435045317, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87081958, Length=390, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17538396, Length=375, Percent_Identity=25.8666666666667, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71985184, Length=396, Percent_Identity=24.2424242424242, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17506239, Length=312, Percent_Identity=24.3589743589744, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17508101, Length=315, Percent_Identity=24.7619047619048, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534899, Length=379, Percent_Identity=25.8575197889182, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17569725, Length=371, Percent_Identity=23.989218328841, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17533517, Length=262, Percent_Identity=21.7557251908397, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71985192, Length=318, Percent_Identity=24.2138364779874, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355753, Length=341, Percent_Identity=26.099706744868, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363737, Length=396, Percent_Identity=23.7373737373737, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24666513, Length=377, Percent_Identity=23.6074270557029, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356377, Length=338, Percent_Identity=24.8520710059172, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24646207, Length=307, Percent_Identity=26.0586319218241, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.1.62

Molecular weight: Translated: 40215; Mature: 40215

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNLNDEQRQLADSLSRLLADHYSFEKRRALTRPEATCEPAIWTHLAALGVTALGIPEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
FGGLGGGTQDRLPVMQAFGRALAIEPYLASAVLGTTAVREGGSSAQQAELLPSLASGEMR
HCCCCCCCCCCHHHHHHCCHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
IAWAHDEAAGRHAPLWVETTARSGPDGWRLDGAKALVLHGGAVSRIVVTARTRGKAQDED
EEEECCCCCCCCCCEEEEECCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCC
GLALFLVDPQADGVTRRAYRLVDDTPAVELTLRNAQACPLGDVSDSVRATAVIRATQAAG
CEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
IAAACADMVGTMETALQLTTDYVRTRKQFGRPIGDYQSMRHRIADMCVSLELARSMAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAGDDPSGDDGLAELSRAKLVIGRHARTLCHAAIQSHGGIGLTEEYAVGHCLRRVHVLD
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLFGDCDAHTRWLAGQLG
HHHCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNFNLNDEQRQLADSLSRLLADHYSFEKRRALTRPEATCEPAIWTHLAALGVTALGIPEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
FGGLGGGTQDRLPVMQAFGRALAIEPYLASAVLGTTAVREGGSSAQQAELLPSLASGEMR
HCCCCCCCCCCHHHHHHCCHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
IAWAHDEAAGRHAPLWVETTARSGPDGWRLDGAKALVLHGGAVSRIVVTARTRGKAQDED
EEEECCCCCCCCCCEEEEECCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCC
GLALFLVDPQADGVTRRAYRLVDDTPAVELTLRNAQACPLGDVSDSVRATAVIRATQAAG
CEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
IAAACADMVGTMETALQLTTDYVRTRKQFGRPIGDYQSMRHRIADMCVSLELARSMAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAGDDPSGDDGLAELSRAKLVIGRHARTLCHAAIQSHGGIGLTEEYAVGHCLRRVHVLD
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLFGDCDAHTRWLAGQLG
HHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pimeloyl-CoA; NAD+

Specific reaction: pimeloyl-CoA + NAD+ = 6-carboxyhex-2-enoyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]