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The map label for this gene is paaG [C]

Identifier: 116694319

GI number: 116694319

Start: 411860

End: 412690

Strand: Direct

Name: paaG [C]

Synonym: H16_B0365

Alternate gene names: 116694319

Gene position: 411860-412690 (Clockwise)

Preceding gene: 116694318

Following gene: 116694323

Centisome position: 14.14

GC content: 68.11

Gene sequence:

>831_bases
ATGCAGGAAGACATCATCACGCTGCGCGAAGCGCGCCTGGAAATCCGCGACGGCATCGCCGAGTTCACGCATACCCAGGC
CAAACGGCGCAATCCGCTCTCGCTCGAACTGCGCGAGGACTACACCGACATGCTGGATCGCGTGGAGGGCAACCCCGACG
TGCGCGCGCTGATCATCACCGGCGAGGGCGGGGTATTCAGCGCCGGCGGCGACCTGCGGGGCATGAAGGCGCGTATCGAC
AACCCGGATGCTGCGCAGCACACGCCCGACGCCACGCGCCGCCGCGTGCTCGTGCTGCACGACTGGGTCAGCCGGCTGCG
CAGCCTGGAGATACCCGTCATCGCCGCCGTGGACGGTCCCGCGGTGGGCGCGGGCATGGGGCTGGCCCTGATTGCCGACT
TTGTGCTGGCGTCGTCGCGCGCATTCTTCGGCATGTCGTTCGCCAAGGTAGGCATGATCCCCGATATGGCCGCCGCCTAT
TTCCTGCCGCGCGTGGTCGGCATGGCCGCCGCCAAGGACCTGATGATGACGGCGCGCCGCGTCAGCGCCGAGGAAGCCAA
AGACCTCGGCATCGTGTATTCCATCTTCCCGCACGAGACGCTTGCGGAGCAGGCACGCCGGTTTGCCCGGCGCTTCGTCG
ACGGCCCGCCCGAGGCGCTCGGCATGACCAAGCGCATGCTCAACAGCGCTTTCGAGACGACCTACGCCACGTTCGTCGAG
CTCGAAGCCGCCGCGCAGGCCGTGGTCACCACCACGCCGTTCCACCGGGAGGCCGTGCAACGCTTCGTCGATGGCCAGCC
CGGGCGTTATGACTGGGATCGCCAGGCGTGA

Upstream 100 bases:

>100_bases
ATTCCGACCTGCGACGAACTGGTGTCGCGCATGGTGGCCGAGTGCCGCAGCGCGTTGCAGGCATCGCTCAACCGCTTTGA
CGCCTGAGGAGGATCACGAG

Downstream 100 bases:

>100_bases
GCCACCGGCATAGGTCCGTTTAGAGACGAGCCGTCAAGAAGGGGGGAAAGCAAAGCCGCAGCCGAAAACGGCTGCGGCTT
TCGCATTACACCAGCTGCAT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 4-CBA-CoA dehalogenase; 4-CBCoA dehalogenase; 4-chlorobenzoyl-CoA dehalogenase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQEDIITLREARLEIRDGIAEFTHTQAKRRNPLSLELREDYTDMLDRVEGNPDVRALIITGEGGVFSAGGDLRGMKARID
NPDAAQHTPDATRRRVLVLHDWVSRLRSLEIPVIAAVDGPAVGAGMGLALIADFVLASSRAFFGMSFAKVGMIPDMAAAY
FLPRVVGMAAAKDLMMTARRVSAEEAKDLGIVYSIFPHETLAEQARRFARRFVDGPPEALGMTKRMLNSAFETTYATFVE
LEAAAQAVVTTTPFHREAVQRFVDGQPGRYDWDRQA

Sequences:

>Translated_276_residues
MQEDIITLREARLEIRDGIAEFTHTQAKRRNPLSLELREDYTDMLDRVEGNPDVRALIITGEGGVFSAGGDLRGMKARID
NPDAAQHTPDATRRRVLVLHDWVSRLRSLEIPVIAAVDGPAVGAGMGLALIADFVLASSRAFFGMSFAKVGMIPDMAAAY
FLPRVVGMAAAKDLMMTARRVSAEEAKDLGIVYSIFPHETLAEQARRFARRFVDGPPEALGMTKRMLNSAFETTYATFVE
LEAAAQAVVTTTPFHREAVQRFVDGQPGRYDWDRQA
>Mature_276_residues
MQEDIITLREARLEIRDGIAEFTHTQAKRRNPLSLELREDYTDMLDRVEGNPDVRALIITGEGGVFSAGGDLRGMKARID
NPDAAQHTPDATRRRVLVLHDWVSRLRSLEIPVIAAVDGPAVGAGMGLALIADFVLASSRAFFGMSFAKVGMIPDMAAAY
FLPRVVGMAAAKDLMMTARRVSAEEAKDLGIVYSIFPHETLAEQARRFARRFVDGPPEALGMTKRMLNSAFETTYATFVE
LEAAAQAVVTTTPFHREAVQRFVDGQPGRYDWDRQA

Specific function: Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA and 4-bromobenzoyl-CoA, but not 4-fluorobenzoyl-CoA. Inactive towards crotonyl-CoA, alpha-methylcrotonyl-CoA and beta-methylcrotonyl- CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=29.6296296296296, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=27.0142180094787, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=27.0142180094787, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI260274832, Length=211, Percent_Identity=27.0142180094787, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=32.0197044334975, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4757968, Length=205, Percent_Identity=23.4146341463415, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI49355787, Length=205, Percent_Identity=23.4146341463415, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI116235487, Length=187, Percent_Identity=25.668449197861, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31542718, Length=200, Percent_Identity=30.5, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4757966, Length=205, Percent_Identity=22.9268292682927, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=205, Percent_Identity=22.9268292682927, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=205, Percent_Identity=22.9268292682927, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538573, Length=205, Percent_Identity=22.9268292682927, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=213, Percent_Identity=29.5774647887324, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI221307494, Length=227, Percent_Identity=24.2290748898678, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=227, Percent_Identity=24.2290748898678, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221136753, Length=227, Percent_Identity=24.2290748898678, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI20127408, Length=255, Percent_Identity=25.0980392156863, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=252, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI213417737, Length=252, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694520, Length=230, Percent_Identity=23.4782608695652, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=34.8, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=34.5, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787659, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=27.3092369477912, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=24.3781094527363, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=25.4901960784314, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=22.3107569721116, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=266, Percent_Identity=28.9473684210526, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17535521, Length=202, Percent_Identity=28.2178217821782, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=227, Percent_Identity=29.0748898678414, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=30.4721030042918, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=209, Percent_Identity=28.2296650717703, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=24.0816326530612, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=25.3456221198157, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=23.2142857142857, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=214, Percent_Identity=27.5700934579439, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=25.7028112449799, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=25.7028112449799, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=26.9961977186312, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24654903, Length=213, Percent_Identity=26.2910798122066, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=31.1320754716981, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=201, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=159, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =3.8.1.7 [H]

Molecular weight: Translated: 30483; Mature: 30483

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEDIITLREARLEIRDGIAEFTHTQAKRRNPLSLELREDYTDMLDRVEGNPDVRALIIT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEE
GEGGVFSAGGDLRGMKARIDNPDAAQHTPDATRRRVLVLHDWVSRLRSLEIPVIAAVDGP
CCCCEEECCCCCCCCCEECCCCCCCCCCCCHHHHEEHHHHHHHHHHHCCCCCEEEEECCC
AVGAGMGLALIADFVLASSRAFFGMSFAKVGMIPDMAAAYFLPRVVGMAAAKDLMMTARR
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAEEAKDLGIVYSIFPHETLAEQARRFARRFVDGPPEALGMTKRMLNSAFETTYATFVE
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEAAAQAVVTTTPFHREAVQRFVDGQPGRYDWDRQA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQEDIITLREARLEIRDGIAEFTHTQAKRRNPLSLELREDYTDMLDRVEGNPDVRALIIT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEEEEE
GEGGVFSAGGDLRGMKARIDNPDAAQHTPDATRRRVLVLHDWVSRLRSLEIPVIAAVDGP
CCCCEEECCCCCCCCCEECCCCCCCCCCCCHHHHEEHHHHHHHHHHHCCCCCEEEEECCC
AVGAGMGLALIADFVLASSRAFFGMSFAKVGMIPDMAAAYFLPRVVGMAAAKDLMMTARR
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAEEAKDLGIVYSIFPHETLAEQARRFARRFVDGPPEALGMTKRMLNSAFETTYATFVE
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEAAAQAVVTTTPFHREAVQRFVDGQPGRYDWDRQA
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1351742; 1610806 [H]