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The map label for this gene is gudD [H]

Identifier: 116694087

GI number: 116694087

Start: 145079

End: 146434

Strand: Direct

Name: gudD [H]

Synonym: H16_B0127

Alternate gene names: 116694087

Gene position: 145079-146434 (Clockwise)

Preceding gene: 116694085

Following gene: 116694088

Centisome position: 4.98

GC content: 69.03

Gene sequence:

>1356_bases
ATGACCCCAACCGCCGCCCCCATGGCGTCCGCCCACACCCCGGTCGTGACCGAACTGACCGTGGTGCCCGTGGCCGGGCA
TGACAGCATGCTGATGAACCTGTCCGGCGCCCATGGCCCGTACTTCACTCGCAATATCGTGATCGTGCGCGACAGCGCCG
GCCACACCGGCGTGGGCGAGGTCCCGGGCGGCGAGGGCATCCGCCAGACGCTGGAAGACGCGCGGCCGCTGCTGGTGGGC
CAGCCCATCGGCCAGTACCAGTCCATCCTGAACCGCGTGCGCGCGGCATTCGCCAGCCGCGATGCCGGCGGCCGCGGCCT
GCAGACCTTTGACCTGCGCATCACCATCCATGCGGTGACGGCGCTCGAGGCGGCGCTGCTCGACCTGCTCGGCCAGCACC
TCGAGGTGCCGGTGGCCGCACTGCTCGGCGAAGGCCAGCAGCGCGACGCCGTGGAGATGCTCGGCTACCTGTTCTATATC
GGCGATCGCCAGCGCACCACGCTGGACTACCGCACCGAGCCCGATGCCGACAACGCGTGGTTCCGCCTGCGCAATGAAGG
GGCGATGACGCCCGAGGGCGTGGTGCGCCTGGCCGAAGCCGCTTACGAGCGCTATGGATTCAACGACTTCAAGCTCAAGG
GCGGCGTGCTGAGCGGCAACGAAGAGATGGAAGCGATCCTGGCGCTGGCCGAACGCTTCCCGAAGGCGCGCATAACGCTG
GATCCCAACGGCGCCTGGTCGCTGGCAGAGGCCGTGCGCCTGTGCCGCGACAAGCGCGGCGTGCTGGCCTATGCCGAAGA
CCCGTGCGGCGCCGAAGACGGCTATTCCGGGCGCGAGATCATGGCCGAATTCCGCACTGCCACCGGCCTGCCCACCGCCA
CCAACATGATCGCCACCGACTGGCGCCAGATGGGGCACGCCGTGCGGCTGCAGTCGGTCGACATCCCGCTGGCCGACCCG
CACTTCTGGACCATGCAGGGCTCGGTGCGCGTGGCGCAGATGTGCGCCGAATGGGGCCTGACCTGGGGCTCGCACTCGAA
CAACCACTTCGATATTTCGCTGGCGATGTTCACCCACGTGGCCGCCGCCGCGCCGGGGCGCGTCACTGCCATCGACACGC
ACTGGATCTGGCAGGACGGCGAAAACCTGACCCGCAACCCGCTGCGGATCGAAGGCGGCCTGGTGCAGGTGCCCAAGACC
CCGGGCCTGGGCGTGGAGCTCGACATGGATGCCCTGGCGCGCGCGCACGAGCTGTACCAGCGCAAGGGCCTGGGCGCACG
CGACGATGCCGCGGCGATGCAATTCCTGATCCCCGGCTGGAAGTTCAACAACAAGGCGCCCTGCATGGTGCGCTGA

Upstream 100 bases:

>100_bases
ACTGGGTTATCAGTCATACGATGACAGATAACATACGCGAAGTGATGGTCCAGTGAACAATACTGCGCCCGCCATCGGAA
ATGCCGGAGACAACCCTGAG

Downstream 100 bases:

>100_bases
CGCAGTACCGGCAACGCCCAACCGGCCGCTGCCGTGATCGGCACGGCTGGCTGATCTGATAAAGACACTGCAGGAGACAA
CGATGTACACGTTCCAACGC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTPTAAPMASAHTPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVIVRDSAGHTGVGEVPGGEGIRQTLEDARPLLVG
QPIGQYQSILNRVRAAFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDAVEMLGYLFYI
GDRQRTTLDYRTEPDADNAWFRLRNEGAMTPEGVVRLAEAAYERYGFNDFKLKGGVLSGNEEMEAILALAERFPKARITL
DPNGAWSLAEAVRLCRDKRGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLQSVDIPLADP
HFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGENLTRNPLRIEGGLVQVPKT
PGLGVELDMDALARAHELYQRKGLGARDDAAAMQFLIPGWKFNNKAPCMVR

Sequences:

>Translated_451_residues
MTPTAAPMASAHTPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVIVRDSAGHTGVGEVPGGEGIRQTLEDARPLLVG
QPIGQYQSILNRVRAAFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDAVEMLGYLFYI
GDRQRTTLDYRTEPDADNAWFRLRNEGAMTPEGVVRLAEAAYERYGFNDFKLKGGVLSGNEEMEAILALAERFPKARITL
DPNGAWSLAEAVRLCRDKRGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLQSVDIPLADP
HFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGENLTRNPLRIEGGLVQVPKT
PGLGVELDMDALARAHELYQRKGLGARDDAAAMQFLIPGWKFNNKAPCMVR
>Mature_450_residues
TPTAAPMASAHTPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVIVRDSAGHTGVGEVPGGEGIRQTLEDARPLLVGQ
PIGQYQSILNRVRAAFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLEVPVAALLGEGQQRDAVEMLGYLFYIG
DRQRTTLDYRTEPDADNAWFRLRNEGAMTPEGVVRLAEAAYERYGFNDFKLKGGVLSGNEEMEAILALAERFPKARITLD
PNGAWSLAEAVRLCRDKRGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLQSVDIPLADPH
FWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGENLTRNPLRIEGGLVQVPKTP
GLGVELDMDALARAHELYQRKGLGARDDAAAMQFLIPGWKFNNKAPCMVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=75.2272727272727, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=447, Percent_Identity=61.744966442953, Blast_Score=552, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49221; Mature: 49089

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTAAPMASAHTPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVIVRDSAGHTGVGE
CCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCC
VPGGEGIRQTLEDARPLLVGQPIGQYQSILNRVRAAFASRDAGGRGLQTFDLRITIHAVT
CCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHH
ALEAALLDLLGQHLEVPVAALLGEGQQRDAVEMLGYLFYIGDRQRTTLDYRTEPDADNAW
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEECCCCCCCCCCE
FRLRNEGAMTPEGVVRLAEAAYERYGFNDFKLKGGVLSGNEEMEAILALAERFPKARITL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCHHHHHHHHHHHHCCCEEEEE
DPNGAWSLAEAVRLCRDKRGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAVRLQSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGENLTRNPLRIEGGLVQVPKTPGLGVELDMDALARAHELYQ
HHCCCCCEEEEEEEEEEECCCCCCCCCEEEECCEEECCCCCCCCEEECHHHHHHHHHHHH
RKGLGARDDAAAMQFLIPGWKFNNKAPCMVR
HCCCCCCCHHHHHHHCCCCEECCCCCCCEEC
>Mature Secondary Structure 
TPTAAPMASAHTPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVIVRDSAGHTGVGE
CCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCC
VPGGEGIRQTLEDARPLLVGQPIGQYQSILNRVRAAFASRDAGGRGLQTFDLRITIHAVT
CCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHH
ALEAALLDLLGQHLEVPVAALLGEGQQRDAVEMLGYLFYIGDRQRTTLDYRTEPDADNAW
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEECCCCCCCCCCE
FRLRNEGAMTPEGVVRLAEAAYERYGFNDFKLKGGVLSGNEEMEAILALAERFPKARITL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCHHHHHHHHHHHHCCCEEEEE
DPNGAWSLAEAVRLCRDKRGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAVRLQSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGENLTRNPLRIEGGLVQVPKTPGLGVELDMDALARAHELYQ
HHCCCCCEEEEEEEEEEECCCCCCCCCEEEECCEEECCCCCCCCEEECHHHHHHHHHHHH
RKGLGARDDAAAMQFLIPGWKFNNKAPCMVR
HCCCCCCCHHHHHHHCCCCEECCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]