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The map label for this gene is garL [H]

Identifier: 116691134

GI number: 116691134

Start: 3431226

End: 3432011

Strand: Reverse

Name: garL [H]

Synonym: Bcen2424_3116

Alternate gene names: 116691134

Gene position: 3432011-3431226 (Counterclockwise)

Preceding gene: 116691135

Following gene: 116691133

Centisome position: 98.51

GC content: 71.5

Gene sequence:

>786_bases
ATGAGCACGCTCACCAATTCCCTCAAACAACGCCTGCGCGACGGTGACGAGCCGCTGTACGGCCTGTGGCTGTCGCTCGG
CAGCGACTCGGCCGCGGAAGCGCTCGCGCACGCCGGTTACGACTGGCTCTGCATCGACATGGAGCATGCGCCGAACGACA
GTCGCGACGTGGCGTCGCAACTGCGTGCGATCGCCGCGGCGCACCTGCCGAGCGAGCCGGTCGTGCGCGTGCCGGCGCGC
GAGCCGTGGCTCGTGAAGCGCGCGCTCGACGCCGGCGCGCGCACGCTGATGTTCCCGTGTATCGAGACGTCCGATGACGC
CGCGCACGCGGTGCGGCTCACGCGCTTCCCGTCGCCCGACACACCCGACGGGCTGCGCGGTGTCGCAGGCATGGTGCGCG
CGGCGGCGTTCGGCATGCGCCGCGACTACCTGCAGACGGCGAACGCGCAAGTCGCAGTGGTCGTGCAGGTCGAATCGGCG
CGCGGCGTCGACGAAGTCGAGCGGATCGCGGCGACGCCCGGCGTCGATTGCCTGTTCGTCGGCCCGGCCGATCTCGCGGC
GAGCCTCGGGCATCTCGGCGACATCCGCCACCCGGACGTCGAAACCGCGATGGCGCGCGTGCTCGCGGCCGGCAAGCAGG
CAGGCGTCGCGGTCGGCATCTTCGCGGGCGACACGGCGGCCGCGCGCCAGTATCGCGACGCCGGTTACCGGATGATCACG
GTATCGGCCGACGTGAGCTGGCTATTGCGCGCGACGCGGCAGGCGTTGCAGGAGGTACGGTCATGA

Upstream 100 bases:

>100_bases
CCGCCGCCGTGCTTCGGTTGCCCGGCGGTATGCATTCCGCTTTCCGCTGCGCCTGTTGCTTGTCCCGTTCGATTGCCGTT
CCGTTCCGGAGGCCGTATCG

Downstream 100 bases:

>100_bases
ACGGCGCAGACGCTGCGTGCCGCGGGCGCGCGCGGATCGGGACGATGGTCGGCCTGTGGGCCGTGTGTGCGGTTGCCGCC
GCGCAGGGCGCGCCGCAAGC

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSTLTNSLKQRLRDGDEPLYGLWLSLGSDSAAEALAHAGYDWLCIDMEHAPNDSRDVASQLRAIAAAHLPSEPVVRVPAR
EPWLVKRALDAGARTLMFPCIETSDDAAHAVRLTRFPSPDTPDGLRGVAGMVRAAAFGMRRDYLQTANAQVAVVVQVESA
RGVDEVERIAATPGVDCLFVGPADLAASLGHLGDIRHPDVETAMARVLAAGKQAGVAVGIFAGDTAAARQYRDAGYRMIT
VSADVSWLLRATRQALQEVRS

Sequences:

>Translated_261_residues
MSTLTNSLKQRLRDGDEPLYGLWLSLGSDSAAEALAHAGYDWLCIDMEHAPNDSRDVASQLRAIAAAHLPSEPVVRVPAR
EPWLVKRALDAGARTLMFPCIETSDDAAHAVRLTRFPSPDTPDGLRGVAGMVRAAAFGMRRDYLQTANAQVAVVVQVESA
RGVDEVERIAATPGVDCLFVGPADLAASLGHLGDIRHPDVETAMARVLAAGKQAGVAVGIFAGDTAAARQYRDAGYRMIT
VSADVSWLLRATRQALQEVRS
>Mature_260_residues
STLTNSLKQRLRDGDEPLYGLWLSLGSDSAAEALAHAGYDWLCIDMEHAPNDSRDVASQLRAIAAAHLPSEPVVRVPARE
PWLVKRALDAGARTLMFPCIETSDDAAHAVRLTRFPSPDTPDGLRGVAGMVRAAAFGMRRDYLQTANAQVAVVVQVESAR
GVDEVERIAATPGVDCLFVGPADLAASLGHLGDIRHPDVETAMARVLAAGKQAGVAVGIFAGDTAAARQYRDAGYRMITV
SADVSWLLRATRQALQEVRS

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=234, Percent_Identity=43.1623931623932, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1788578, Length=218, Percent_Identity=44.0366972477064, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27896; Mature: 27765

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTNSLKQRLRDGDEPLYGLWLSLGSDSAAEALAHAGYDWLCIDMEHAPNDSRDVASQ
CCHHHHHHHHHHHCCCCCCEEHEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRAIAAAHLPSEPVVRVPAREPWLVKRALDAGARTLMFPCIETSDDAAHAVRLTRFPSPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHEEEEEECCCCC
TPDGLRGVAGMVRAAAFGMRRDYLQTANAQVAVVVQVESARGVDEVERIAATPGVDCLFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEE
GPADLAASLGHLGDIRHPDVETAMARVLAAGKQAGVAVGIFAGDTAAARQYRDAGYRMIT
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
VSADVSWLLRATRQALQEVRS
EECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLTNSLKQRLRDGDEPLYGLWLSLGSDSAAEALAHAGYDWLCIDMEHAPNDSRDVASQ
CHHHHHHHHHHHCCCCCCEEHEEECCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRAIAAAHLPSEPVVRVPAREPWLVKRALDAGARTLMFPCIETSDDAAHAVRLTRFPSPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHEEEEEECCCCC
TPDGLRGVAGMVRAAAFGMRRDYLQTANAQVAVVVQVESARGVDEVERIAATPGVDCLFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEE
GPADLAASLGHLGDIRHPDVETAMARVLAAGKQAGVAVGIFAGDTAAARQYRDAGYRMIT
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
VSADVSWLLRATRQALQEVRS
EECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA