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The map label for this gene is gacA [H]

Identifier: 116691073

GI number: 116691073

Start: 3369526

End: 3370182

Strand: Direct

Name: gacA [H]

Synonym: Bcen2424_3055

Alternate gene names: 116691073

Gene position: 3369526-3370182 (Clockwise)

Preceding gene: 116691072

Following gene: 116691074

Centisome position: 96.72

GC content: 65.75

Gene sequence:

>657_bases
ATGAGCCTGAACATCCTGCTCGTGGACGATCACGCGATCGTCCGGCAAGGGATCCGCCAGTTGCTGATCGACCGCGGCGT
CGCCCGCGACGTGACCGAGGCCGAGAACGGCGGCGATGCGATGGCCGCCGTCGACAAGCAGGAATTCGACGTGATCCTGC
TCGACATCTCGCTGCCCGACACGAACGGCATCGAGGTGCTCAAGCGCCTCAAGCGCAAGCTGACGCGCACGCCGGTGCTG
ATGTTCTCGATGTATCGCGAGGATCAGTACGCGGTGCGCGCGCTGAAGGCCGGCGCGGCCGGCTACCTGTCGAAGACGGT
CAACGCCGCGCAGATGATCGGCGCGATCCAGCAGGTCGCGGCCGGCCGCAAGTACGTGAGCCCCGCGATGGCCGAGGCGC
TCGCCGAATACGTGTCGTTCGAGAACGAACCGCTGCCGCACGAGAAGCTGTCCGATCGCGAATACCAGACGCTCTGCATG
CTCGCGTCGGGCAAGCGGCTCACCGACATCGCGAACACGCTGTCGCTGTCGGTGAAGACGGTGAGCGTGTACCGCTCGCG
GCTGCTGGAGAAGATGCGGCTGTCGAACAACGCGGAACTGACGTTCTACGTGATGAGCAACCGGCTCGTCGACATGGCGC
CCACGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
GCAACCGGCCGCGGCACGCGCGTCACCGCGCGCTTCGCGTGGGCTTCGCTGCTGGCGGCGCCCGTCGCGGCACGCCGCAC
GTCGCTTTCGTGAGGTCGCG

Downstream 100 bases:

>100_bases
TTCCGGCCGAATCGAACTCGTCCGATCCTGCGCGCGGCGCCCGCCGCGCCGCGCTCGGCCGTGCCCGTTTCGCGCCCGGT
CTGCTCCCGTTTCGCGCGCA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAMAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLTRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGA

Sequences:

>Translated_218_residues
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAMAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLTRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGA
>Mature_217_residues
SLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAMAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLTRTPVLM
FSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCML
ASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGA

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=33.8095238095238, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=37.3134328358209, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=28.0193236714976, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=30.9178743961353, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1786747, Length=213, Percent_Identity=30.5164319248826, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787473, Length=210, Percent_Identity=32.8571428571429, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788191, Length=112, Percent_Identity=33.0357142857143, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=35.5769230769231, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=107, Percent_Identity=37.3831775700935, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24098; Mature: 23967

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAMAAVDKQEFDVILLDISLPD
CCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCCCCCEEEEEEECCC
TNGIEVLKRLKRKLTRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
SLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAMAAVDKQEFDVILLDISLPD
CEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCCCCCEEEEEEECCC
TNGIEVLKRLKRKLTRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]